Abstract
Freshly isolated CD8+ T cells, but not CD4+ T cells, contained 20–30% of asialo GM1+ (ASGM1+) T cells which were distinct from ASGM1+NK1.1+ natural killer cells. This novel ASGM1+CD8+ T cell subpopulation showed a strong proliferative response to interlenkin‐12 (IL‐12) in the presence of IL‐2. Culture of ASGM1+CD8+ T cells with IL‐12 plus IL‐2 allowed the generation of anomalous killer T cells concomitantly with the accumulation of cytolytic molecules. Moreover, ASGM1+CD8+ T cells produced high levels of interferon‐γ (IFN‐γ), but not IL‐4, upon stimulation with IL‐12 plus IL‐2. Such immune responses were not observed in ASGM1− CD8+ T cell snbpopulations constituting the majority of CD8+ T cells. These results demonstrated that ASGM1+CD8+ T cells are a novel subpopulation of IL‐12‐responsive and IFN‐γ‐producing killer T cell precursors.
Keywords: IL‐12, Asialo GM1, CD8+ T cell, Killer T cell precursor, IFN‐γ
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