Abstract
We introduced the interleukin‐2 (IL‐2) gene into mouse renal cell carcinoma (RenCa) in order to examine the mechanism of tumor rejection. IL‐2 gene‐transfected RenCa (RenCa/IL‐2Hi) exhibited marked retardation of tumor growth when implanted in a syngeneic host. Growth retardation of RenCa/IL‐2Hi was also observed in athymic nude mice even after depletion of natural killer (NK) cells by treatment with anti‐asialo GM1 antibody. Histological analysis of RenCa/IL‐2Hi tumors disclosed non‐specific inflammatory changes in syngeneic hosts. Co‐injection of Bacillus Calmette Guerin with RenCa/IL‐2Hi considerably enhanced the anti‐tumor effects. Taken together, these findings strongly suggest that in situ IL‐2 production leads to tumor rejection through non‐specific inflammatory responses without participation of T cells and NK cells. On the other hand, the syngeneic mice that had rejected RenCa/IL‐2Hi acquired immunity against parental RenCa, suggesting possible participation of memory T cells in the second rejection of the tumor.
Keywords: Renal cell carcinoma, Interleukin‐2, Tumor rejection, Inflammation
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