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Japanese Journal of Cancer Research : Gann logoLink to Japanese Journal of Cancer Research : Gann
. 1997 Jul;88(7):669–677. doi: 10.1111/j.1349-7006.1997.tb00435.x

High Expression of Uridine Diphosphate‐galactose: Lc3Cer βl‐3 Galactosyltransferase in Human Uterine Endometrial Cancer‐derived Cells as Measured by Enzyme‐linked Immunosorbent Assay and Thin‐layer Chromatography‐immunostaining

Junko Yoshiki 1, Kaneyuki Kubushiro 1,✉, Katsumi Tsukazaki 1, Yasuhiro Udagawa 1, Shiro Nozawa 1, Masao Iwamori 2
PMCID: PMC5921478  PMID: 9310140

Abstract

We have developed a new procedure for the selective determination of β1‐3 and β1‐4 galactosyltrans‐ferases with Lc3Cer as the substrate and the microsomes of fetal and adult porcine livers as the enzyme sources. This method was based on the detection of such products as Lc4Cer for β1‐3 galactosyltransferase (β1‐3GT) and nLc4Cer for β1‐4 galactosyltransferase (β1‐4GT), with monoclonal anti‐Lc4Cer and anti‐nLc4Cer antibodies, respectively. This method thus enabled us to differentiate the activity of β1‐3GT from that of 4bT1‐4GT with a high degree of sensitivity. The method was then used to determine the activities of both enzymes in human gynecological carcinoma‐derived cells. Four of the five cell lines derived from uterine endometrial cancer expressed significantly high levels of specific activity of β1‐3GT among the cell lines examined, while their β1‐4GT activities were less than 20% of that for β1‐3GT in the endometrial carcinoma‐derived cells. On the other hand, a higher specific activity of β1‐4GT than that of β1‐3GT was detected in the cell lines derived from uterine cervical and ovarian cancers. These findings were thus found to correlate closely with the rate of expression of Lc4Cer‐ and nLc4Cer‐based carbohydrate chains in the cell lines based on the results of immunohistochemical staining.

Keywords: Galactosyltransferase, TLC‐immunostaining, ELISA, Uterine endometrial cancer, Carbohydrate chain

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