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. 1998 Mar;89(3):262–268. doi: 10.1111/j.1349-7006.1998.tb00557.x

Establishment of Epstein‐Barr Virus‐positive Human Gastric Epithelial Cell Lines

Masako Tajima 1, Masato Komuro 2, Kota Okinaga 3
PMCID: PMC5921807  PMID: 9600119

Abstract

We have established two Epstein‐Barr virus (EBV)‐positive cell lines, GT38 and GT39, derived from human gastric tissues of two patients bearing gastric carcinoma. Both cell lines were positive for cytokeratin, an epithelial marker, but not for lymphocyte‐related markers. Unlike GT39 cell line, GT38 cells lacked the property of contact inhibition. EBV genome was detected in both cell lines. The cell lines were positive for latent membrane protein 1, and EBV‐determined nuclear antigen 1 (EBNA1). EBNA2 was also detected in GT38. These cell lines should be useful for studying the interaction of EBV with gastric epithelial cells and its role in gastric carcinogenesis.

Keywords: Epstein‐Barr virus, Gastric carcinoma, Cell line, LMP1, EBNA1

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REFERENCES

  • 1. ) Niedobitek , G. and Young , L. S.Epstein‐Barr virus persistence and virus‐associated tumours . Lancet , 343 , 333 – 335 ( 1994. ). [DOI] [PubMed] [Google Scholar]
  • 2. ) Fahraeus , R. , Fu , H. L. , Ernberg , I. , Finke , J. , Rowe , M. , Klein , G. , Falk , K. , Nilsson , E. , Yadav , M. , Busson , P. , Tursz , T. and Kallin , B.Expression of Epstein‐Barr virus‐encoded proteins in nasopharyngeal carcinoma . Int. J. Cancer , 42 , 329 – 338 ( 1988. ). [DOI] [PubMed] [Google Scholar]
  • 3. ) Shimakage , M. , Oka , T. , Shinka , T. , Kurata , A. , Sasagawa , T. and Yutsudo , M.Involvement of Epstein‐Barr virus expression in testicular tumors . J. Urol. , 156 , 253 – 257 ( 1996. ). [PubMed] [Google Scholar]
  • 4. ) Leung , S. Y. , Chung , L. P. , Yuen , S. T. , Ho , C. M. , Wong , M. P. and Chan , S. Y.Lymphoepithelial carcinoma of the salivary gland: in situ detection of Epstein‐Barr virus . J. Clin. Pathol. , 48 , 1022 – 1027 ( 1995. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 5. ) Wong , M. P. , Chung , L. P. , Yuen , S. T. , Leung , S. Y. , Chan , S. Y. , Wang , E. and Fu , K. H.In situ detection of Epstein‐Barr virus in non‐small cell lung carcinomas . J. Pathol. , 177 , 233 – 240 ( 1995. ). [DOI] [PubMed] [Google Scholar]
  • 6. ) Harn , H. J. , Chang , J. Y. , Wang , M. W. , Ho , L. I. , Lee , H. S. , Chiang , J. H. and Lee , W. H.Epstein‐Barr virus‐associated gastric adenocarcinoma in Taiwan . Hum. Pathol. , 26 , 267 – 271 ( 1995. ). [DOI] [PubMed] [Google Scholar]
  • 7. ) Shibata , D. and Weiss , L. M.Epstein‐Barr virus‐associated gastric adenocarcinoma . Am. J. Pathol. , 140 , 769 – 774 ( 1992. ). [PMC free article] [PubMed] [Google Scholar]
  • 8. ) Tokunaga , M. , Land , C. E. , Uemura , Y. , Tokudome , T. , Tanaka , S. and Sato , E.Epstein‐Barr virus in gastric carcinoma . Am. J. Pathol. , 143 , 1250 – 1254 ( 1993. ). [PMC free article] [PubMed] [Google Scholar]
  • 9. ) Yuen , S. T. , Chung , L. P. , Leung , S. Y. , Luk , I. S. , Chan , S. Y. and Ho , J.In situ detection of Epstein‐Barr virus in gastric and colorectal adenocarcinomas . Am. J. Surg. Pathol. , 18 , 1158 – 1163 ( 1994. ). [DOI] [PubMed] [Google Scholar]
  • 10. ) Leoncini , L. , Vindigni , C. , Megha , T. , Funto , I. , Pacenti , L. , Musaro , M. , Renieri , A. , Seri , M. , Anagnostopoulos , J. and Tosi , P.Epstein‐Barr virus and gastric cancer: data and unanswered questions . Int. J. Cancer , 53 , 898 – 901 ( 1993. ). [DOI] [PubMed] [Google Scholar]
  • 11. ) Matsunou , H. , Konishi , F. , Hori , H. , Ikeda , T. , Sasaki , K. , Hirose , Y. and Yamamichi , N.Characteristics of Epstein‐Barr virus‐associated gastric carcinoma with lymphoid stroma in Japan . Cancer , 77 , 1998 – 2004 ( 1996. ). [DOI] [PubMed] [Google Scholar]
  • 12. ) Oda , K. , Tamaru , J. , Takenouchi , T. , Mikata , A. , Nunomura , M. , Saitoh , N. , Sarashina , H. and Nakajima , N.Association of Epstein‐Barr virus with gastric carcinoma with lymphoid stroma . Am. J. Pathol. , 143 , 1063 – 1071 ( 1993. ). [PMC free article] [PubMed] [Google Scholar]
  • 13. ) Mori , M. , Watanabe , M. , Tanaka , S. , Mimori , K. , Kuwano , H. and Sugimachi , K.Epstein‐Barr virus‐associated carcinomas of the esophagus and stomach . Arch. Pathol. Lab. Med. , 118 , 998 – 1001 ( 1994. ). [PubMed] [Google Scholar]
  • 14. ) Nakamura , S. , Ueki , T. , Yao , T. , Ueyama , T. and Tsuneyoshi , M.Epstein‐Barr virus in gastric carcinoma with lymphoid stroma. Special reference to its detection by the polymerase chain reaction and in situ hybridization in 99 tumors, including a morphologic analysis . Cancer , 73 , 2239 – 2249 ( 1994. ). [DOI] [PubMed] [Google Scholar]
  • 15. ) Imai , S. , Koizumi , S. , Sugiura , M. , Tokunaga , M. , Uemura , Y. , Yamamoto , N. , Tanaka , S. , Sato , E. and Osato , T.Gastric carcinoma: monoclonal epithelial malignant cells expressing Epstein Barr virus latent infection protein . Proc. Natl. Acad. Sci. USA , 91 , 9131 – 9135 ( 1994. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 16. ) Ott , G. , Kirchner , T. and Muller‐Hermelink , H. K.Monoclonal Epstein‐Barr virus genomes but lack of EBV‐related protein expression in different types of gastric carcinoma . Histopathology , 25 , 323 – 329 ( 1994. ). [DOI] [PubMed] [Google Scholar]
  • 17. ) Gulley , M. L. , Pulitzer , D. R. , Eagan , P. A. and Schneider , B. G.Epstein‐Barr virus infection is an early event in gastric carcinogenesis and is independent of bcl‐2 expression and p53 accumulation . Hum. Pathol. , 27 , 20 – 27 ( 1996. ). [DOI] [PubMed] [Google Scholar]
  • 18. ) Pittaluga , S. , Loke , S. L. , So , K. C. , Cheung , K. N. and Ma , L.Clonal Epstein‐Barr virus in lymphoepithelioma‐like carcinoma of the stomach: demonstration of viral genome by in situ hybridization and Southern blot analysis . Mod. Pathol. , 5 , 661 – 664 ( 1992. ). [PubMed] [Google Scholar]
  • 19. ) Fukayama , M. , Hayashi , Y. , Iwasaki , Y. , Chong , J. , Ooba , T. , Takizawa , T. , Koike , M. , Miyaki , S. , Mizutani , M. and Hirai , K.Epstein‐Barr virus‐associated gastric carcinoma and Epstein‐Barr virus infection of the stomach . Lab. Invest. , 71 , 73 – 81 ( 1994. ). [PubMed] [Google Scholar]
  • 20. ) Wang , D. , Liebowitz , D. and Kieff , E.An EBV membrane protein expressed in immortalized lymphocytes transforms established rodent cell . Cell , 43 , 831 – 840 ( 1985. ). [DOI] [PubMed] [Google Scholar]
  • 21. ) Dawson , C. W. , Rickinson , A. B. and Young , L. S.Epstein‐Barr virus latent membrane protein inhibits human epithelial cell differentiation . Nature , 344 , 777 – 780 ( 1990. ). [DOI] [PubMed] [Google Scholar]
  • 22. ) Fahraeus , R. , Rymo , L. , Rhim , J. S. and Klein , G.Morphological transformation of human keratinocytes expressing the LMP gene of Epstein‐Barr virus . Nature , 345 , 447 – 449 ( 1990. ). [DOI] [PubMed] [Google Scholar]
  • 23. ) Sekiguchi , M. , Sakakibara , K. and Fuji , G.Establishment of cultured cell lines derived from a human gastric carcinoma . Jpn. J. Exp. Med. , 48 , 61 – 68 ( 1978. ). [PubMed] [Google Scholar]
  • 24. ) Barranco , S. C. , Townsend , C. M. , Casartelli , C. , Jr. , Macik , B. G. , Burger , N. L. , Boerwinkle , W. R. and Gourley , W. K.Establishment and characterization of an in vitro model system for human adenocarcinoma of the stomach . Cancer Res. , 43 , 1703 – 1709 ( 1983. ). [PubMed] [Google Scholar]
  • 25. ) Takano , Y. and Kato , Y.Epstein‐Barr virus association with early cancers found together with gastric medullary carcinomas demonstrating lymphoid infiltration . J. Pathol. , 175 , 39 – 44 ( 1995. ). [DOI] [PubMed] [Google Scholar]
  • 26. ) Rowlands , D. C. , Ito , M. , Mangham , D. C. , Reynolds , G. , Herbst , H. , Hallissey , M. T. , Fielding , J. W. , Newbold , K. M. , Jones , E. L. , Young , L. S. and Niedobitek , G.Epstein‐Barr virus and carcinomas: rare association of the virus with gastric adenocarcinomas . Br. J. Cancer , 68 , 1014 – 1019 ( 1993. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 27. ) Shin , W. S. , Kang , M. W. , Kang , J. H. , Choi , M. K. , Ahn , B. M. , Kim , J. K. , Sun , H. S. and Min , K. W.Epstein‐Barr virus‐associated gastric adenocarcinomas among Koreans . Am. J. Clin. Pathol. , 105 , 174 – 181 ( 1996. ). [DOI] [PubMed] [Google Scholar]
  • 28. ) Liebowitz , D. , Wang , D. and Kieff , E.Orientation and patching of the latent infection membrane protein encoded by Epstein‐Barr virus . J. Virol. , 58 , 233 – 237 ( 1986. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 29. ) Martin , J. and Sugden , B.Transformation by the oncogenic latent membrane protein correlates with its rapid turnover, membrane localization, and cytoskeletal association . J. Virol. , 65 , 3246 – 3258 ( 1991. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 30. ) Cordier‐Bussat , M. , Calender , A. , Vuillaume , M. , Bornkamm , G. W. and Lenoir , G. M.Expression of the Epstein‐Barr virus (EBV) latent membrane protein is tightly regulated, independently of EB nuclear antigen 2 and of EBV integration or copy number . Virus Res. , 27 , 55 – 69 ( 1993. ). [DOI] [PubMed] [Google Scholar]
  • 31. ) Li , Q. X. , Young , L. S. , Niedobitek , G. , Dawson , C. W. , Birkenbach , M. , Wang , F. and Rickinson , A. B.Epstein‐Barr virus infection and replication in a human epithelial cell system . Nature , 356 , 347 – 350 ( 1992. ). [DOI] [PubMed] [Google Scholar]
  • 32. ) Baichwal , V. R. and Sugden , B.Posttranslational processing of an Epstein‐Barr virus‐encoded membrane protein expressed in cells transformed by Epstein‐Barr virus . J. Virol. , 61 , 866 – 875 ( 1987. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 33. ) Mann , K. P. and Thorley‐Lawson , D.Posttranslational processing of the Epstein‐Barr virus‐encoded p63/LMP protein . J. Virol. , 61 , 2100 – 2108 ( 1987. ). [DOI] [PMC free article] [PubMed] [Google Scholar]

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