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. 1998 Jul;89(7):783–788. doi: 10.1111/j.1349-7006.1998.tb03284.x

Fluorescence in situ Hybridization Analysis of 12;21 Translocation in Japanese Childhood Acute Lymphoblastic Leukemia

Minenori Eguchi‐Ishimae 1, Mariko Eguchi 1, Kimio Tanaka 1, Kazuko Hamamoto 2, Misao Ohki 3, Kazuhiro Ueda 4, Nanao Kamada 1,✉
PMCID: PMC5921884  PMID: 9738986

Abstract

Fluorescence in situ hybridization (FISH) analysis was applied to detect t(12;21) using two yeast artificial chromosome probes and cosmid probes covering the TEL(ETV6) and the AML1 gene to clarify the incidence of abnormality of t(12;21) in Japanese childhood acute lymphoblastic leukemia (ALL). We detected seven TEL/AML1 fusion positive patients (9.5%), all of whom were diagnosed as B‐lineage ALL, among 74 childhood ALL. On the other hand, no TEL/AML1 fusion positive patients were found among 37 adult ALL. The incidence among Japanese seemed to be lower than that among other nations. Of the seven patients with the TEL/AML1 fusion, five exhibited normal karyotype, one was t(8;12)(q11;p13), i(21q) and the remaining one exhibited a near‐triploid karyotype in conventional G‐banding. The FISH method clearly demonstrated that all patients with the TEL/AML1 fusion had subpopulations of leukemic cells with deletion of the normal TEL allele, which is significant for understanding the progression of leukemia with t(12;21).

Keywords: key words, 12;21 translocation, FISH, TEL gene

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