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. 1998 Aug;89(8):849–854. doi: 10.1111/j.1349-7006.1998.tb00639.x

Sequence Analysis of Genes Encoding Rodent Homologues of the Human Tumor‐rejection Antigen SART‐1

Masashi Gotoh 1, Shigeki Shichijo 2,✉, Tomoaki Hoshino 2, Yasuhisa Imai 2, Toshihiro Imaizumi 3, Yoshiko Inoue 3, Hideo Takasu 1, Takashi Yamaoka 1, Kyogo Itoh 2,3
PMCID: PMC5921909  PMID: 9765622

Abstract

Human SART‐1 (hSART‐1) gene encodes a 125 kD protein with a leucine‐zipper motif expressed in the nucleus of all proliferating cells, and a 43 kD protein expressed in the cytosol of most epithelial cancers. In this study, two rodent genes (rSART‐1 and mSART‐1) homologous to hSART‐1 were cloned from cDNA libraries of murine brain and a rat tumor cell line, respectively. mSART‐1 and rSART‐1 were highly homologous to hSART‐1 with 86% and 84% identity at the nucleotide level, and 95% and 91% at the protein level, respectively. The leucine zipper domain and two basic amino acid portions that bind DNA, as well as peptide sequences recognized by human cyto‐toxic T lymphocytes (CTLs), were all conserved in these rodent genes. Nuclear protein homologous to the 125 kD hSART‐1800 protein, but not to the 43 kD cytosol SART‐1259 protein, was detectable with specific antibody in the nuclear fractions of rodent tumor cell lines, and normal rodent fetal liver and testis. These rodent genes should be a novel tool for studies on the biological roles of the SART‐1 gene, and also in the construction of animal models of specific immuno‐therapy using SART‐1 gene products.

Keywords: key words, Tumor rejection antigen, Cytotoxic T lymphocyte, Rodent genes

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This work was supported in part by Grants‐in‐Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan, and from the Ministry of Health and Welfare of Japan.

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