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. 2018 Apr 10;9(27):18949–18969. doi: 10.18632/oncotarget.24855

Figure 10. HK1 silencing increases the susceptibility to 2-DG inhibition.

Figure 10

(A) MTT cell growth assay of HK1-knocked down cells cultured in the presence of 2-DG. Cells as indicated were loaded in 96-well plates in the presence of various concentrations of 2-DG as labelled and then carried out MTT cell growth assay according to standard protocols. (B) Colony formation assay of HK1-silenced cells cultured in the presence of 2-DG. Cells as indicated were seeded in 6-well plates in the presence of different concentrations of 2-DG as indicated for 6 or 10 days. After incubation, colonies were stained, imaged and enumerated. * indicates incubation for 10 days. (C) Cell apoptosis assay of HK1-knocked down cells grown in the presence of 2-DG. Cells as indicated were cultured in 6-well plates in the presence of different doses of 2-DG as labelled for 3 days. After incubation, cells were harvested, fixed and subjected to a cell apoptosis assay using the FITC Annexin V Apoptosis Detection Kit I according to the manufacturer’s protocols. Fluorescence intensities of propidium iodide and annexin V-FITC stained cells were analysed using a flow cytometer. The plotted data are averaged from three independent experiments and the bars represent mean ± SD.