Figure 5. HK1 knockdown induced EMT switch accelerates tumor malignancy in vivo.

(A) In vivo cancer growth assay of HK1-silenced cells. Cells as indicated were subcutaneously inoculated into the back of NOD/SCID mice for 20 or 60 days. Mice were culled and tumors were excised and analysed. (B) In vivo cancer metastasis assay of HK1-inhibited cells. Cells as indicated were intravenously injected into the tail vein of NOD/SCID mice for 20 days. Mice were culled and examined for tumor metastasis. Red arrowheads indicate the heart. (C) Histological and immunohistochemical staining of the lung and heart in the in vivo tumor metastasis assay. Experiments were performed using H&E staining and an antibody specific for vimentin.