Table 1.
Primers used in this study to complete the genome sequence of the PM14 isolate.
| Primer Name | Sequence (5′-3′) | Genome Coordinates | Amplicon Size (nt) |
|---|---|---|---|
| Capillo-mume-5Race2 # | CCTTGCATGGTTGTTGTTGAAGTCCTCCC | 251–223 | 252 |
| Capillo-mume-F1 | AACAACAACCATGCAAGGTTTGAG | 234–257 | 503 |
| Capillo-mume-R1 | GCTAGAACACACTTAGGCCGCAA | 736–714 | |
| Capillo-mume-F2 | GGAATGTTGATACATACAGACA | 1629–1650 | 742 |
| Capillo-mume-R2 | CGTCTGAGCCTAATCCATACAC | 2370–2349 | |
| Capillo-mume-F3 | TGGATTTATTGAACTTCTCATAC | 2831–2853 | 406 |
| Capillo-mume-R3 | CGTCACAATCACACCAAATCTG | 3236–3215 | |
| Capillo-mume-F6 | GATGTACGAGGATTCAGTGG | 4034–4053 | 451 |
| Capillo-mume-R6 | AATGAGGGAGTTAGAAACACC | 4484–4464 | |
| Capillo-mume-R7 ~ | ACCAACTGTTATGACAGATTC | 5117–5097 | 1084 |
| LD-PolyT @ | CACTGGCGGCCGCTCGAGCATGTAC(T)25NN | ||
| Capillo-mume-LD | GAGCACCATTGGAGGGTGTGT | 7462–7482 | 183 |
| LD-Prime | CACTGGCGGCCGCTCGAGCATGTAC |
# This reverse primer was used in conjunction with the universal primer provided by the 5’ RACE kit (Takara Bio Europe/Clontech, Saint-Germain-en-Laye, France); ~ This reverse primer was used in conjunction with the Capillo-mume-F6 primer; @ This primer was used for the complementary DNA synthesis prior amplification with the primer pair Capillo-mume-LD and LD-Prime.