Figure 1.

Overview of popular image processing and analyses procedures. Comparison of graphical user interface (GUI) and command line interface (CLI) solutions, which can be used for image post-processing, including denoising, and deconvolution. GUI and CLI are further used for object segmentation in 2D and 3D, particle tracking, for example virions and cells, complex feature detection and extraction by pattern recognition, clustering, multiparametric classification, or inference. GUI and CLI are also used for specialized problems, representing specialized software for assessing particular biological phenotypes. Thick lines denote primary applications of a software/framework for a particular problem. Dashed lines denote secondary applications.