Table 1.
Antibodies used for flow cytometry.
| Cell Receptor | Species Against | Source Antibody Clone |
Isotype | Dilution |
|---|---|---|---|---|
| CD4 | Anti-feline | Southern Biotech Clone 3-4F4 |
Mouse IgG1 | 1:100 |
| CD8 | Anti-feline | Southern Biotech Clone fCD8 |
Mouse IgG1 | 1:100 |
| CD134 | Anti-feline | Serotec Clone 7D6 |
Mouse IgG1 | 1:10 |
| B220 | Anti-mouse | Southern Biotech Clone RA3-6B2 |
Rat IgG2a | 1:100 |
| FAS | Anti-feline | R&D Systems Clone 431014 |
Mouse IgG1 | 1:500 |
| CD21 | Anti-human | BD Pharmingen Clone B-ly4 |
Mouse IgG1 | 1:5 |
| CD45RA | Anti-feline | Calvin Johnson Auburn University |
Mouse IgG1 | 1:7 |
| MHC II (HLA-DR, DP, DQ) | Anti-human | BD Pharmingen Clone TÜ39 |
Mouse IgG1 | 1:10 |
| CD25 | Anti-feline | Wayne Thompkins North Carolina State University |
Mouse IgG2a | 1:20 |
| CD14 | Anti-human | Bio-Rad Clone TÜK4 |
Mouse IgG1 | 1:20 |
FAS: death receptor (CD95); MHC: major histocompatibility complex; HLA-DR: Human Leukocyte Antigen-antigen D Related; DP: DP chain; DQ: DQ chain.