Significance
For robust contraction of skeletal muscles, the L-type calcium channel acts as a key molecule by transducing membrane depolarization to calcium release from the sarcoplasmic reticulum. Proper intracellular localization of L-type calcium channels at the junctional membrane complex where the plasma membranes are closely apposed to the membranes of the sarcoplasmic reticulum is necessary for this process. Junctophilins are known to stabilize the structure of the junctional membrane complex by bridging the plasma membrane and the sarcoplasmic membrane. We report that junctophilins recruit L-type calcium channels to the junctional membrane through physical interaction with the CaV1.1 subunits of the channels. This protein–protein interaction at triads ensures efficient contraction in differentiated adult skeletal muscle.
Keywords: skeletal muscle, dihydropyridine receptor, junctophilin, triad, ryanodine receptor
Abstract
Close physical association of CaV1.1 L-type calcium channels (LTCCs) at the sarcolemmal junctional membrane (JM) with ryanodine receptors (RyRs) of the sarcoplasmic reticulum (SR) is crucial for excitation–contraction coupling (ECC) in skeletal muscle. However, the molecular mechanism underlying the JM targeting of LTCCs is unexplored. Junctophilin 1 (JP1) and JP2 stabilize the JM by bridging the sarcolemmal and SR membranes. Here, we examined the roles of JPs in localization and function of LTCCs. Knockdown of JP1 or JP2 in cultured myotubes inhibited LTCC clustering at the JM and suppressed evoked Ca2+ transients without disrupting JM structure. Coimmunoprecipitation and GST pull-down assays demonstrated that JPs physically interacted with 12-aa residues in the proximal C terminus of the CaV1.1. A JP1 mutant lacking the C terminus including the transmembrane domain (JP1ΔCT) interacted with the sarcolemmal/T-tubule membrane but not the SR membrane. Expression of this mutant in adult mouse muscles in vivo exerted a dominant-negative effect on endogenous JPs, impairing LTCC–RyR coupling at triads without disrupting JM morphology, and substantially reducing Ca2+ transients without affecting SR Ca2+ content. Moreover, the contractile force of the JP1ΔCT-expressed muscle was dramatically reduced compared with the control. Taken together, JPs recruit LTCCs to the JM through physical interaction and ensure robust ECC at triads in skeletal muscle.
L-type calcium channels (LTCCs) play a central role in excitation–contraction coupling (ECC) of skeletal muscle (1). The skeletal muscle LTCC is composed of pore-forming CaV1.1 and ancillary β1, α2δ, and γ subunits (2). The voltage-sensitive domain of CaV1.1 detects action potentials traversing the muscle fiber membrane (sarcolemma) and opens ryanodine receptors (RyRs) in the adjacent sarcoplasmic reticulum (SR) through its II–III loop and β1 subunits to release Ca2+ into the cytoplasm (3). In skeletal muscle, LTCCs and RyRs are clustered at triad junctions where invaginations of the sarcolemmal membrane called transverse tubules (T-tubules) are closely juxtaposed to two terminal cisternae of the SR (4, 5). Association of a single cistern of the SR with T-tubules or plasma membrane, called a diad or peripheral coupling, respectively, is also present in several types of excitable cells, including cardiac myocytes. These membrane structures are collectively referred to as junctional membrane (JM) complexes. Although the proper localization of LTCCs vis-à-vis RyRs at triads is essential for ECC in striated muscles, the molecular mechanism of this targeting is still elusive.
Myotubes of CaV1.1-deficient dysgenic (mdg) mice are a valuable tool to investigate the function and localization of LTCCs in muscle (6–10). Using myotubes differentiated from the immortalized dysgenic myoblast cell line GLT, we and others have identified the motifs necessary for the JM targeting of LTCCs in the C terminus of CaV1.1 and cardiac CaV1.2 subunits (8, 10). However, it is still unknown how these motifs recruit LTCCs to the JM.
Junctophilins (JPs) are molecules that stabilize the JM complex by bridging the sarcolemmal and SR membranes via their N-terminal lipid-binding membrane occupation and recognition nexus (MORN) motif and C-terminal transmembrane domain, respectively (11). Four members of the JP family (JP1–JP4) have been identified in the mammalian genome. JP1 is expressed in skeletal muscle, JP2 in skeletal and cardiac muscle (11), and JP3 and JP4 in the brain (12). Golini et al. (13) demonstrated that JPs physically interact with both LTCCs and RyRs in skeletal muscle. This report also showed that transfection of a siRNA against JPs disrupted the normal punctate distributions of LTCCs and RyRs indicative of JM localization in C2C12 myotubes. In cardiac myocytes, JP2 physically interacts with the LTCC CaV1.2 subunit and modulates the Ca2+ current (14). Thus, in addition to bridging the sarcolemmal and SR membranes, JPs may physically interact with LTCCs and thereby directly support LTCC–RyR coupling in cultured striated muscle.
In this study, we first confirmed that JPs support LTCC–RyR coupling and ECC in cultured myotubes. Biochemical analyses demonstrated that JPs physically interact with the proximal C termini of CaV1.1 subunits and that disruption of this interaction dislocates LTCCs out of the JM. Then, we transduced a JP1 mutant lacking its C terminus including transmembrane domain (JP1ΔCT) in adult mouse tibialis anterior (TA) and flexor digitorum brevis (FDB) muscles using adenoassociated virus (AAV) vectors. This mutant was previously shown to interact with the sarcolemmal membrane but not the SR membrane (11). Interestingly, JP1ΔCT targeted LTCCs over the entire sarcolemma, disturbed LTCC–RyR coupling in triads, and significantly reduced evoked Ca2+ transients and the contractile force of muscles without disrupting the triad structure or reducing SR Ca2+ content. Thus, we provide compelling evidence that JPs recruit LTCCs to precise locations at triads through physical interaction and ensure robust ECC in adult skeletal muscle.
Results
Inhibition of LTCC and RyR JM Targeting by JP Knockdown.
JPs are localized to the JM in skeletal myocytes and myotubes (11, 15). We performed immunocytochemistry on myotubes differentiated from a CaV1.1-lacking GLT cell line to confirm colocalization of JPs, LTCCs, and RyRs. In these GLT-derived myotubes, transduced green fluorescent protein (GFP)-CaV1.1 and endogenous RyR, JP1, and JP2 showed punctate colocalization, indicating that they all accumulated at the JM (Fig. S1A). We then introduced a siRNA against JP1 and/or JP2 into GLT-derived myotubes to assess the role of JPs in the JM targeting of LTCCs and RyRs. Western blotting showed that all three tested siRNAs against either JP1 or JP2 effectively and selectively suppressed expression of JP1 or JP2 (Fig. S1B). We used JP1 siRNA #2 and JP2 siRNA #1 for subsequent experiments. Immunocytochemistry also showed the effectiveness and selectivity of these siRNAs in GLT-derived myotubes (Fig. S1 C and D). It is noteworthy that JP1 knockdown did not inhibit JP2 clustering and vice versa, indicating that either JP1 or JP2 alone can form JM complexes and that knockdown of either alone does not disrupt the JM.
Nevertheless, transfection of a siRNA against JP1 or JP2 significantly inhibited the JM targeting of GFP-CaV1.1 in GLT myotubes (Fig. 1 A and B). JM targeting of endogenous CaV1.1 was also suppressed in C2C12 myotubes by knockdown of JP1 or JP2 (Fig. S2 A and B). These results suggest that, in addition to creating the JM, JP1 and JP2 may function to directly recruit plasma membrane LTCCs. In contrast, the JM targeting of RyRs was inhibited by JP2 but not by JP1 siRNA in both GLT and C2C12 myotubes (Fig. 1 and Fig. S2), suggesting that JM localization of RyRs is determined solely by JP2. Cotransfection of siRNA-resistant JP1 or JP2 constructs rescued the inhibition of the JM targeting of CaV1.1 and RyRs (Fig. S2C).
Effect of JP knockdown on ECC in Myotubes.
The effect of JP knockdown on LTCC ionic and gating currents in C2C12 myotubes was examined. Knockdown of JP2 but not JP1 significantly reduced LTCC ionic currents (Fig. 2 A and B). However, expression of CaV1.1 protein was not affected by transfection of siRNAs against JPs (Fig. 2C). Moreover, neither JP1 nor JP2 siRNA affected gating currents (Fig. 2 D and E), indicating that JPs did not affect the membrane expression of LTCCs.
Nevertheless, knockdown of JP1 or JP2 significantly reduced the number of C2C12 myotubes exhibiting twitch Ca2+ transients in response to field stimulation (Fig. 2F). Moreover, the peak amplitude of Ca2+ transients in responding cells was also significantly reduced by JP1 or JP2 siRNA (Fig. 2 G and H). However, these siRNAs did not affect cyclopiazonic acid (CPA)-induced Ca2+ release from the SR (Fig. 2 I and J), indicating that knockdown of JPs did not affect the SR Ca2+ content. These results indicate that JP1 and JP2 siRNAs may inhibit the efficient coupling of LTCCs and RyRs.
Physical Interaction of JPs with the Proximal C Terminus of CaV1.1.
A physical interaction of JPs with LTCCs and RyRs was previously reported (14, 16). We confirmed this interaction by a coimmunoprecipitation assay using mouse skeletal muscle microsomes (Fig. 3A). We henceforth focused on the molecular mechanism and physiological significance of the interaction between LTCCs and JPs. We first performed a GST pull-down assay to identify the JP-binding motif (JBM) of the LTCC. The cytoplasmic N terminus, I–II loop, II–III loop, III–IV loop, proximal C terminus, and distal C terminus of CaV1.1 were purified as GST-fused recombinant proteins using a bacterial expression system (Fig. S3A). The result showed that the proximal C terminus (PCT) binds to JP1 and JP2 (Fig. 3B). To narrow down the binding motif, we constructed several recombinant proteins bearing different PCT fragments and repeated the GST pull-down assay (Fig. 3C). Unfortunately, the region between fragments #2 and #3 could not be examined because the corresponding protein could not be solubilized under any conditions we tested (Fig. 3C). Through these experiments, however, we could narrow down the JBM to a stretch of 12-aa residues (i.e., #11, amino acids 1,595–1,606) (Fig. 3C).
Contribution of the JBM of CaV1.1 to JM Targeting.
We compared the amino acid sequence of the JBM of CaV1.1 with that of the corresponding regions of cardiac CaV1.2 and neuronal CaV2.1 subunits across different species (Fig. 4A). Multiple alignments indicated that the amino acid sequence of the JBM was well conserved in CaV1.1 and cardiac CaV1.2, but a similar sequence was not present anywhere in neuronal CaV2.1. We purified these regions of CaV1.2 and CaV2.1 as GST-proteins, and once again performed the pull-down assay. As expected, recombinant CaV1.1 and CaV1.2, but not CaV2.1, bound to JPs (Fig. 4C).
To identify the crucial amino acid residues in the JBM, we conducted alanine scanning and performed a GST pull-down assay (Fig. S3A). This experiment revealed that the binding capacities of I1597A, R1599A, R1600A, L1604A, and F1605A mutants were clearly lower than that of the wild type (Fig. 4B). We introduced three representative mutations, R1596A, which caused partial inhibition, and R1600A and R1605A, which caused total inhibition, into the full-length CaV1.1 and expressed them in GLT myotubes. Immunocytochemical analysis showed that the R1600A and R1605A mutations, but not the R1596A mutation, significantly decreased the JM targeting of CaV1.1 compared with the wild type (Fig. 4D and Fig. S3B). Expression of the channel proteins and gating charge movement were not significantly different between wild type and R1600A-transfected myotubes, indicating that the mutant normally localized in plasma membranes (Fig. 4 E–G). Expression of R1600A did not affect the JM targeting of JP1, JP2, and RyR (Fig. S3C). In contrast, the Ca2+ transients in response to field stimulation were significantly reduced in R1600A-transfected myotubes compared with wild-type controls (Fig. 4 H–J). These results suggest that physical binding of CaV1.1 to JPs is necessary for LTCC–RyR coupling and ECC.
Physiological Outcome of in Vivo Overexpression of a C Terminus-Deleted JP1 Mutant in Differentiated Muscles of Living Mice.
Takeshima et al. (11) showed that a C terminus including a transmembrane domain-deleted mutant of JP1 diffusely localized to the plasma membrane of Xenopus oocytes and Madin–Darby canine kidney cells, indicating that the mutant can interact with the sarcolemmal membrane but not the SR membrane. We prepared a similar C terminus-deleted mutant of JP1 with 3×FLAG tag in the C terminus (JP1ΔCT-FLAG). Note that JP1ΔCT-FLAG lacking the C-terminal epitope was not recognized by the anti-JP1 antibody used in this study. This is advantageous because endogenous JP1 and exogenous JP1ΔCT-FLAG can be separately identified with anti-JP1 and anti-FLAG antibodies, respectively (Fig. S3D).
In GLT myotubes, transiently expressed JP1ΔCT-FLAG was not specifically clustered to the JM but was diffusely localized over the entire plasma membrane (Fig. 5A). In the same myotubes, the JM targeting of coexpressed GFP-CaV1.1 was significantly inhibited (Fig. 5A), indicating that JP1ΔCT-FLAG lacks the capacity to guide GFP-CaV1.1 to the JM. On the contrary, JM localization of LTCCs was not affected by expression of a negative control FLAG-PLCδPH, an unrelated protein also known to diffusely localize to the entire plasma membrane (Fig. 5A). Protein expression and membrane localization of CaV1.1 were not affected by JP1ΔCT-FLAG transfection (Fig. 5 B–D). JM targeting of JP1, JP2, and RyR was also not affected by JP1ΔCT-FLAG expression (Fig. S3E). These results suggest that JP1ΔCT-FLAG elicits a dominant-negative effect on the JM targeting of LTCCs and can be utilized as a tool to disrupt LTCC–RyR coupling in living muscles.
Therefore, we constructed an AAV vector carrying JP1ΔCT-FLAG. Twenty days after direct intramuscular injection of the virus into the FDB muscle of mice, expression of JP1ΔCT-FLAG was observed in >80% of isolated fibers (Fig. S4A). Immunocytochemical analysis revealed that JP1ΔCT-FLAG was equally distributed in T-tubule and sarcolemmal membranes in the low-level expression fibers (∼30% of positive fibers). In the major population of JP1ΔCT-FLAG–expressed fibers, the mutant was more strongly localized to the sarcolemmal membrane than the T-tubule membrane (∼70% of positive fibers) (Fig. 5E and Fig. S4B). Interestingly, abundant LTCC signals were observed in the sarcolemma of JP1ΔCT-FLAG–expressing fibers, but not in control fibers (Fig. 5E and Fig. S4C). The localizations of JP1, JP2, and RyRs were not altered by JP1ΔCT-FLAG expression (Fig. S4D). Although the results clearly indicated that JP1ΔCT-FLAG changed LTCC localization, a considerable amount of LTCC signals still remained in the T-tubules. In contrast to myotubes, punctate distribution of CaV1.1 was not detected in the T-tubules of adult FDB fibers by our immunocytochemical analysis. Therefore, we performed a proximity ligation assay (PLA) to reveal whether JP1ΔCT disturbed the coupling of LTCCs with JPs and RyRs in whole cells, including sarcolemma and T-tubules. PLA is a technique that detects an interaction of two molecules in situ using specific antibodies and probes labeled by short DNA strands. PLA revealed that exogenous JP1ΔCT-FLAG strongly interacted with CaV1.1, whereas a much weaker interaction between JP1ΔCT-FLAG and RyRs was observed (Fig. S4E). The PLA assay also revealed that interactions between CaV1.1 and RyRs were significantly inhibited by JP1ΔCT-FLAG (Fig. 5F). Inhibition of physical interaction between CaV1.1 and JPs by JP1ΔCT-FLAG was also confirmed (Fig. 5G and Fig. S4E). Because JPs and RyRs were much more abundantly expressed in triads than in peripheral coupling (Fig. S4D), these results strongly suggest that JP1ΔCT-FLAG blocked interaction between CaV1.1 and JPs and thereby the coupling between CaV1.1 and RyRs at triads. Interactions of RyRs with JPs were not affected by JP1ΔCT-FLAG expression (Fig. 5G and Fig. S4E). In addition, a significant decrease in the peak amplitude of Ca2+ transients during tetanus was evident in JP1ΔCT-FLAG–expressing fibers (Fig. 5H). However, there was no difference in Ca2+ release from the SR induced by the Ca2+-releasing mixture ionomycin, cyclopiazonic acid, and EGTA (ICE) between control and JP1ΔCT-FLAG–expressing FDB fibers (Fig. 5H), indicating that JP1ΔCT-FLAG did not alter SR Ca2+ content. Thus, these data suggest that JP1ΔCT-FLAG inhibits ECC by disrupting the interaction of CaV1.1 with JPs and RyRs in triads.
Finally, we examined the impact of JP1ΔCT-FLAG overexpression on the contraction of TA muscle in situ. Expression of JP1ΔCT-FLAG was observed in almost all fibers in TA muscles by immunohistochemistry (Fig. S4F). Consistent with the PLA study using FDB fibers, reductions in physical interactions of CaV1.1 and JPs were observed by coimmunoprecipitation (Fig. 5I). No significant difference in cross-sectional area was observed between control and JP1ΔCT-FLAG-AAV–injected muscles (Fig. S4G), and transmission electron microscopic analysis revealed that JP1ΔCT-FLAG did not affect the distance between T-tubule membranes and SR membranes (Fig. S4 H and I), indicating that JP1ΔCT-FLAG did not destroy the JM structure. However, JP1ΔCT-FLAG-AAV significantly decreased the contractile force of muscle at all stimulation frequencies between 1 and 200 Hz (Fig. 5J). These results provide compelling evidence that the precise localization of LTCCs in the JM by JPs and LTCC–RyR coupling at triads is crucial for efficient contraction of skeletal muscle.
Discussion
In this study, we show that knockdown of JP1 or JP2 in myotubes inhibits the clustering of LTCCs in the JM and suppresses electrically evoked Ca2+ transients without disrupting JM structure. JPs physically interacted with the proximal C terminus of CaV1.1, and disruption of this interaction by mutagenesis inhibited the JM clustering of LTCCs.
Because mice lacking JP1 die shortly after birth and JP2 knockouts die in utero (11, 17), it was impossible to analyze the functional significance of skeletal muscle JPs in adulthood with conventional knockout mice. In the present study, we therefore adopted an approach to acutely transduce JP1ΔCT-FLAG-AAV in adult FDB and TA. Fortunately, the approach was not lethal and did not destroy the JM or reduce the SR Ca2+ content, but it selectively disrupted LTCC–RyR coupling at triads and inhibited ECC (Fig. 5). Immunoprecipitation and PLA indicated that JP1ΔCT-FLAG reduced CaV1.1–JP and CaV1.1–RyR interactions to ∼30% (Fig. 5 F and I). Because JPs and RyRs were much more abundantly expressed in triads than in peripheral coupling (Fig. S4D), these results strongly suggest that JP1ΔCT-FLAG blocked interaction between CaV1.1 and JPs and thereby the coupling between CaV1.1 and RyRs at triads. In the JM of skeletal muscle, four CaV1.1 channel molecules are arranged in orthogonal arrays called tetrads that correspond in position to the RyRs. The tetrad formation is critical for skeletal muscle-specific links between LTCCs and RyRs (18). One possibility is therefore that JP1ΔCT-FLAG hampered tetrad formation in triads by inhibiting the interaction between CaV1.1 and JPs. The detailed mechanism of action awaits further analysis; however, it was clear that inhibition of physical interaction of CaV1.1 and JPs by JP1ΔCT (Fig. 5I) leads to prominent defects in ECC. Thus, we provide compelling evidence that, in addition to generating the JM complex, JPs function to directly recruit LTCCs to the JM through protein–protein interaction and support efficient physiological LTCC–RyR coupling in triads.
This effect of JPs is not simply due to suppression of CaV1.1 membrane expression. In fact, suppression of JP1 and/or JP2 did not change the gating charge of LTCCs in C2C12 myotubes (Fig. 2), indicating that JPs are not essential for membrane targeting of LTCCs. In contrast to our study, Golini et al. (13) reported that JP1 and JP2 siRNAs suppressed the expression and gating charge currents of LTCCs. Whereas the siRNAs were transfected at the myotube stage (2 d after differentiation started) in our study, they transfected them at the myoblast stage. It is possible that JPs are also necessary at an early stage of differentiation of the myotubes, and this may have caused a decrease in CaV1.1 expression in the previous study.
JP1ΔCT-FLAG suppressed the interaction of LTCCs and JP1 as well as JP2 (Fig. 5I). Since the JBM in CaV1.1 can interact with both JP1 and JP2, it is possible that JP1ΔCT-FLAG simultaneously inhibited the binding of both JPs to CaV1.1. Although both JP1 and JP2 are necessary for the JM targeting of LTCCs in skeletal muscle, there are functional differences between them. For instance, suppression of JP2, but not JP1, disturbed the JM targeting of RyRs in C2C12 myotubes (Fig. 1). Therefore, JP2 knockdown may more severely decouple CaV1.1 and RyR. RyR transmits a retrograde stimulatory signaling to CaV1.1 through this coupling (19), which may explain why JP2 but not JP1 siRNA significantly decreased LTCC ionic currents in the C2C12 myotubes (Fig. 2). However, it is proposed that JP2 may contribute to the creation of diads and peripheral couplings, while JP1 may contribute to the maturation of diads to triads during skeletal muscle differentiation. Therefore, JP1ΔCT inhibited ECC mainly by disrupting LTCC–JP1 coupling in triads.
To date, there have been several reports analyzing the CaV1 domain responsible for JM targeting. Flucher et al. (8) determined that a 55-aa sequence in the C terminus (amino acids 1,607–1,661) contained the JM targeting signal of CaV1.1. Nakada et al. (10) reported that amino acid residues 1,677–1,708 in the C terminus of cardiac CaV1.2 (corresponding to amino acids 1,551–1,583 of CaV1.1) were necessary for JM targeting. However, the JBM determined in this study (amino acids 1,595–1,606) does not exactly match these JM targeting signals, although it is located immediately proximal to Flucher’s site and immediately distal to Nakada’s site. It was shown that loss of Flucher’s site led to a complete abolition of CaV1.1 JM targeting (8), indicating that this site is also necessary for JM targeting, in addition to our JBM. Thus, the JM targeting of CaV1.1 in skeletal muscle may be regulated by multiple sites and processes. Additional studies are necessary to identify what signal(s) are sufficient for the JM targeting of CaV1.1 in skeletal muscle.
To summarize, we have demonstrated that JP1 and JP2 can physically interact with the cytoplasmic C terminus of the LTCC CaV1.1 subunit. In addition to guiding the formation of JM complexes, JPs localize LTCCs to the JM and enable the channels to efficiently couple with RyRs at triads through this protein–protein interaction. This mechanism is crucial for efficient ECC in differentiated adult skeletal muscle.
Materials and Methods
The detailed materials and methods are described in SI Materials and Methods.
Animals.
All experimental procedures were conducted in accordance with the Guidelines for Animal Experimentation of Shinshu University and approved by the Committee for Animal Experimentation. Isolation of skeletal muscles and AAV injection were performed using 10- to 13-wk-old male C57BL/6 mice.
Cell Culture.
C2C12 and GLT myoblast cell lines were differentiated to myotubes in low serum condition.
Molecular Cloning and AAV Production.
All plasmid vectors were produced by standard molecular biology techniques. For preparation of AAV vectors, the AAVpro Helper Free system (Clontech) was used according to the manufacturer’s protocols.
Immunocytochemistry, Immunohistochemistry, and PLA.
Immunocytochemistry and immunohistochemistry were carried out by standard protocols. PLA was performed with Duolink systems (Sigma-Aldrich). All antibodies used in this study are listed in Table S1.
Patch-Clamp Analysis.
Ionic and gating currents of the LTCC were recorded in the whole-cell configuration at room temperature. Ca+ imaging was conducted with Fluo-4/AM (Dojindo).
Measurement of Contractile Forces.
The contractile forces of TA muscles were measured in vivo according to the methods described in a previous study with minor modification (20).
GST-Fusion Protein Production and Pull-Down Assay.
GST-fusion proteins were produced by a bacterial expression system using BL21 cells.
Preparation of Microsomes.
Gluteus and hindlimb muscles were dissected from mice, and microsomes were prepared. The resulting microsomes were solubilized in lysis buffer and used for Western blotting, immunoprecipitation, and GST pull-down assay.
Immunoprecipitation and Western Blotting.
Immunoprecipitation and Western blotting were conducted as previously described, with minor modifications (21). All antibodies used in this study are listed in Table S1.
Statistical Analysis.
Data are shown as means ± SEM. Statistical significance was evaluated by Student’s unpaired t test. For multiple comparisons, analysis of variance with Bonferroni’s test was used. A value of P < 0.05 was considered to indicate statistical significance.
Note.
During the preparation of this manuscript, Perni et al. (22) reported that CaV1.1, β1a, Stac3, RyR1, and JP2 are sufficient to reproduce the skeletal muscle type ECC in tsA201 cells.
Supplementary Material
Acknowledgments
We are grateful to Prof. Bernhard Flucher (Innsbruck Medical University), Prof. Manfred Grabner (Innsbruck Medical University), and Prof. William Catterall (University of Washington) for kindly providing the cDNA of α1 subunits. We are grateful to Reiko Sakai for secretarial assistance. This work was supported by Grants-in-Aid for Scientific Research 24590271 and 16K08491 from the Ministry of Education, Culture, Sport, Science and Technology of Japan and by The Novartis Foundation (Japan) for the Promotion of Science (to T.N.).
Footnotes
The authors declare no conflict of interest.
This article is a PNAS Direct Submission.
This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1716649115/-/DCSupplemental.
References
- 1.Calderón JC, Bolaños P, Caputo C. The excitation-contraction coupling mechanism in skeletal muscle. Biophys Rev. 2014;6:133–160. doi: 10.1007/s12551-013-0135-x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 2.Arikkath J, Campbell KP. Auxiliary subunits: Essential components of the voltage-gated calcium channel complex. Curr Opin Neurobiol. 2003;13:298–307. doi: 10.1016/s0959-4388(03)00066-7. [DOI] [PubMed] [Google Scholar]
- 3.Tanabe T, Beam KG, Adams BA, Niidome T, Numa S. Regions of the skeletal muscle dihydropyridine receptor critical for excitation-contraction coupling. Nature. 1990;346:567–569. doi: 10.1038/346567a0. [DOI] [PubMed] [Google Scholar]
- 4.Flucher BE, Morton ME, Froehner SC, Daniels MP. Localization of the a1 and a2 subunits of the dihydropyridine receptor and ankyrin in skeletal muscle triads. Neuron. 1990;5:339–351. doi: 10.1016/0896-6273(90)90170-k. [DOI] [PubMed] [Google Scholar]
- 5.Jorgensen AO, Shen AC, Arnold W, Leung AT, Campbell KP. Subcellular distribution of the 1,4-dihydropyridine receptor in rabbit skeletal muscle in situ: An immunofluorescence and immunocolloidal gold-labeling study. J Cell Biol. 1989;109:135–147. doi: 10.1083/jcb.109.1.135. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 6.Powell JA, Petherbridge L, Flucher BE. Formation of triads without the dihydropyridine receptor a subunits in cell lines from dysgenic skeletal muscle. J Cell Biol. 1996;134:375–387. doi: 10.1083/jcb.134.2.375. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 7.Neuhuber B, et al. Association of calcium channel a1S and b1a subunits is required for the targeting of b1a but not of a1S into skeletal muscle triads. Proc Natl Acad Sci USA. 1998;95:5015–5020. doi: 10.1073/pnas.95.9.5015. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 8.Flucher BE, Kasielke N, Grabner M. The triad targeting signal of the skeletal muscle calcium channel is localized in the COOH terminus of the a1S subunit. J Cell Biol. 2000;151:467–478. doi: 10.1083/jcb.151.2.467. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 9.Obermair GJ, et al. The Ca2+ channel a2d1 subunit determines Ca2+ current kinetics in skeletal muscle but not targeting of a1S or excitation-contraction coupling. J Biol Chem. 2005;280:2229–2237. doi: 10.1074/jbc.M411501200. [DOI] [PubMed] [Google Scholar]
- 10.Nakada T, et al. The proximal C-terminus of a1C subunits is necessary for junctional membrane targeting of cardiac L-type calcium channels. Biochem J. 2012;448:221–231. doi: 10.1042/BJ20120773. [DOI] [PubMed] [Google Scholar]
- 11.Takeshima H, Komazaki S, Nishi M, Iino M, Kangawa K. Junctophilins: A novel family of junctional membrane complex proteins. Mol Cell. 2000;6:11–22. doi: 10.1016/s1097-2765(00)00003-4. [DOI] [PubMed] [Google Scholar]
- 12.Nishi M, Sakagami H, Komazaki S, Kondo H, Takeshima H. Coexpression of junctophilin type 3 and type 4 in brain. Brain Res Mol Brain Res. 2003;118:102–110. doi: 10.1016/s0169-328x(03)00341-3. [DOI] [PubMed] [Google Scholar]
- 13.Golini L, et al. Junctophilin 1 and 2 proteins interact with the L-type Ca2+ channel dihydropyridine receptors (DHPRs) in skeletal muscle. J Biol Chem. 2011;286:43717–43725. doi: 10.1074/jbc.M111.292755. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 14.Jiang M, et al. JPH-2 interacts with Cai-handling proteins and ion channels in dyads: Contribution to premature ventricular contraction-induced cardiomyopathy. Heart Rhythm. 2016;13:743–752. doi: 10.1016/j.hrthm.2015.10.037. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 15.Barone V, Randazzo D, Del Re V, Sorrentino V, Rossi D. Organization of junctional sarcoplasmic reticulum proteins in skeletal muscle fibers. J Muscle Res Cell Motil. 2015;36:501–515. doi: 10.1007/s10974-015-9421-5. [DOI] [PubMed] [Google Scholar]
- 16.Murphy RM, et al. Ca2+-dependent proteolysis of junctophilin-1 and junctophilin-2 in skeletal and cardiac muscle. J Physiol. 2013;591:719–729. doi: 10.1113/jphysiol.2012.243279. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 17.Ito K, et al. Deficiency of triad junction and contraction in mutant skeletal muscle lacking junctophilin type 1. J Cell Biol. 2001;154:1059–1067. doi: 10.1083/jcb.200105040. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 18.Protasi F, et al. Multiple regions of RyR1 mediate functional and structural interactions with a1S-dihydropyridine receptors in skeletal muscle. Biophys J. 2002;83:3230–3244. doi: 10.1016/S0006-3495(02)75325-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 19.Nakai J, et al. Enhanced dihydropyridine receptor channel activity in the presence of ryanodine receptor. Nature. 1996;380:72–75. doi: 10.1038/380072a0. [DOI] [PubMed] [Google Scholar]
- 20.Cosgrove BD, et al. Rejuvenation of the muscle stem cell population restores strength to injured aged muscles. Nat Med. 2014;20:255–264. doi: 10.1038/nm.3464. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 21.Kashihara T, Nakada T, Kojima K, Takeshita T, Yamada M. Angiotensin II activates CaV1.2 Ca2+ channels through β-arrestin2 and casein kinase 2 in mouse immature cardiomyocytes. J Physiol. 2017;595:4207–4225. doi: 10.1113/JP273883. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 22.Perni S, Lavorato M, Beam KG. De novo reconstitution reveals the proteins required for skeletal muscle voltage-induced Ca2+ release. Proc Natl Acad Sci USA. 2017;114:13822–13827. doi: 10.1073/pnas.1716461115. [DOI] [PMC free article] [PubMed] [Google Scholar]
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