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Japanese Journal of Cancer Research : Gann logoLink to Japanese Journal of Cancer Research : Gann
. 1999 Sep;90(9):914–921. doi: 10.1111/j.1349-7006.1999.tb00835.x

Serum n‐3 Fatty Acids, Fish Consumption and Cancer Mortality in Six Japanese Populations in Japan and Brazil

Minatsu Kobayashi 1,2, Satoshi Sasaki 2, Gerson S Hamada 3, Shoichiro Tsugane 2,✉
PMCID: PMC5926174  PMID: 10551318

Abstract

Japanese people consume significant amounts of long chain n‐3 polyunsaturated fatty acids (PUFAs) derived from fish, but the association of PUFAs with cancer mortality has not been fully investigated. To study geographic differences in n‐3 PUFAs intake, we compared serum fatty acid and dietary fish intake among various Japanese populations having different rates of cancer mortality. The subjects were 50 men from each of five regions in Japan and 47 Japanese men from Sao Paulo, Brazil. All were randomly selected and aged 40 to 49 years. Serum fatty acids were measured by gas chromatography and the frequency of fish intake was obtained by a food frequency questionnaire. Significant geographic differences in serum fatty acid levels (% of total fatty acids) and fish intake (days/4 weeks) were observed. The percentages of serum total PUFA were similar in the six regions, though there was an almost three‐fold difference in n‐3 PUFAs content between Brazil (3.9%) and Akita (10.9%). The frequency of total fish intake corresponded to serum n‐3 PUFAs composition. The relationship between cancer mortality and serum n‐3 PUFAs levels was not clear, though an inverse association between prostate cancer and serum n‐3 PUFAs levels appeared to exist. The results suggest that although serum n‐3 PUFAs varied significantly, the observed geographic difference did not account for the different cancer risks at the population level.

Keywords: Serum fatty acids, Fish consumption, Cancer mortality in Japanese

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REFERENCES

  • 1).Miller , A. , Berrino , F. , Hill , M. , Pietnen , P. , Piboli , E. and Wahrendorf , J.Diet in the aetiology of cancer: a review . Eur. J. Cancer , 30 , 207 – 220 ( 1994. ). [DOI] [PubMed] [Google Scholar]
  • 2).Willett , W. C.Diet and health: what should we eat ? Science , 264 , 532 – 537 ( 1994. ). [DOI] [PubMed] [Google Scholar]
  • 3).Rose , D. P.Dietary fatty acids and cancer . Am. J. Clin. Nutr. , 66 ( Suppl. ), 998 – 1003 ( 1997. ). [DOI] [PubMed] [Google Scholar]
  • 4).Byers , T. and Gieseker , K.Issues in the design and interpretation of studies of fatty acids and cancer in humans . Am. J. Clin. Nutr. , 66 ( Suppl. ), 1541 – 1547 ( 1997. ). [DOI] [PubMed] [Google Scholar]
  • 5).Pariza , M. W.Animal studies: summary, gaps, and future research . Am. J. Clin. Nutr. , 66 ( Suppl. ), 1539 – 1540 ( 1997. ). [DOI] [PubMed] [Google Scholar]
  • 6).Carroll , K. K.Biological effects of fish oils in relation to chronic disease . Lipids , 21 , 731 – 732 ( 1986. ). [DOI] [PubMed] [Google Scholar]
  • 7).Cave , W. T.Dietary n‐3 polyunsaturated fatty acid effects on animal tumorigenesis . FASEB J. , 5 , 2160 – 2166 ( 1991. ). [DOI] [PubMed] [Google Scholar]
  • 8).Organization for Economic Co‐operation and Development . “ Food Consumption Statistics ” pp . 1964 – 1978 ( 1981. ). OECD; , Paris . [Google Scholar]
  • 9).Yamori , Y. , Nara , Y. , Iritani , N. , Workman , R. J. and Inagami , T.Comparison of serum phospholipid fatty acids among fishing and farming Japanese populations and American islanders . J. Nutr. Sci. Vitaminol. , 31 , 417 – 422 ( 1985. ). [DOI] [PubMed] [Google Scholar]
  • 10).Science and Technology Agency . “ Fatty Acids, Cholesterol, Vitamin E Composition Table of Japanese Foods” ( 1990. ). Ishiyaku Shuppan Publ. , Tokyo ( in Japanese ). [Google Scholar]
  • 11).Phillips , R. L.Role of life‐style and dietary habits in risk of cancer among Seventh‐Day Adventists . Cancer Res. , 35 , 3513 – 3522 ( 1975. ). [PubMed] [Google Scholar]
  • 12).Giovannucci , E. and Willett , W. C.Dietary lipids and colon cancer . Princ. Pract. Oncol. Updates , 9 , 1 – 12 ( 1995. ). [Google Scholar]
  • 13).Willett , W. C.Specific fatty acids and risks of breast and prostate cancer: dietary intake . Am. J. Clin. Nutr. , 66 ( Suppl. ), 1557 – 1563 ( 1997. ). [DOI] [PubMed] [Google Scholar]
  • 14).Rose , D. P.Effect of dietary fatty acids on breast and prostate cancers: evidence from in vitro experiments and animal studies . Am. J. Clin. Nutr. , 66 ( Suppl. ), 1513 – 1522 ( 1997. ). [DOI] [PubMed] [Google Scholar]
  • 15).Kohlmeier , L.Biomarkers of fatty acid exposure and breast cancer risk . Am. J. Clin. Nutr. , 66 ( Suppl. ), 1548 – 1556 ( 1997. ). [DOI] [PubMed] [Google Scholar]
  • 16).Tsugane , S. , Gey , F. , Ichinowatari , Y. , Miyajima , Y. , Ishibashi , T. , Matsushima , S. , Hirota , Y. , Inami , T. , Yamaguchi , M. , Karita , K. , Kabuto , M. , Takashima , Y. , Todoroki , H. , Tsuda , M. , Akabane , M. , Furuichi , Y. , Hamada , G. and Watanabe , S.Cross‐sectional epidemiologic study for assessing cancer risks at the population level. 1. Study design and participation rate . J. Epidemiol. 2 , 75 – 81 ( 1992. ). [Google Scholar]
  • 17).Tsugane , S. , Gey , F. , Ichinowatari , Y. , Miyajima , Y. , Ishibashi , T. , Matsushima , S. , Hirota , Y. , Inami , T. , Yamaguchi , M. , Karita , K. , Kabuto , M. , Takashima , Y. , Todoroki , H. , Tsuda , M. , Akabane , M , Furuichi , Y. , Hamada , G. and Watanabe , S.Cross‐sectional epidemiologic study for assessing cancer risks at the population level. 2. Baseline data and correlation analysis . J. Epidemiol. , 2 , 83 – 89 ( 1992. ). [Google Scholar]
  • 18).Tsugane , S. , Hamada , G. , Souza , J. , Gotlieb , S. , Takashima , Y. , Todoroki , H. , Kabuto , M. , Karita , K. , Yamaguchi , M. , Watanabe , S. and Laurenti , R.Lifestyle and health related factors among randomly selected Japanese residents in the city of Sao Paulo, Brazil, and their comparisons with Japanese in Japan . J. Epidemiol. , 4 , 37 – 46 ( 1994. ). [Google Scholar]
  • 19).Breslow , N. and Day , N. E.“Statistical Methods in Cancer Research Vol. II. The Design and Analysis of Cohort Studies ” IARC Sci. Publ. No. 82 , pp . 51 – 61 ( 1987. ). IARC; , Lyon . [PubMed] [Google Scholar]
  • 20).Tsugane , S. , Gotlieb , S. L. , Laurenti , R. , Souza , J. M. and Watanabe , S.Mortality and cause of death among first‐generation Japanese in Sao Paulo, Brazil . Int. J. Epidemiol. , 18 , 647 – 651 ( 1989. ). [DOI] [PubMed] [Google Scholar]
  • 21).Tsugane , S. , Gotlieb , S. L. , Laurenti , R. , Souza , J. M. and Watanabe , S.Cancer mortality among Japanese residents of the city of Sao Paulo, Brazil . Int. J. Cancer , 45 , 436 – 439 ( 1990. ). [DOI] [PubMed] [Google Scholar]
  • 22).Mkheian , E. E. and Shakhbatian , S. H. L.Character of loosely and tightly bound human cerebral gangliosides and methods of preparatory extraction . Vopr. Biokhim. Mozga , 10 , 187 – 191 ( 1975. ). [PubMed] [Google Scholar]
  • 23).SAS Institute Inc. “ SAS/STAT User's Guide ” Version 6.4 , Vol. 2 ( 1989. ). SAS Institute; , Cary , NC . [Google Scholar]
  • 24).Kenneth , J. R. and Sander , G. “ Modern Epidemiology ” 2nd Ed. , pp . 469 – 478 ( 1998. ). Lippincott‐Raven Publ. , Philadelphia . [Google Scholar]
  • 25).Iso , H. , Sato , S. , Folsom , A. R. , Shimamoto , T. , Terao , A. , Munger , R. G. , Kitamura , A. , Konishi , M. , Iida , M. and Komachi , Y.Serum fatty acids and fish intake in rural Japanese, urban Japanese, Japanese American and Caucasian American men . Int. J. Epidemiol. , 18 , 374 – 381 ( 1989. ). [DOI] [PubMed] [Google Scholar]
  • 26).Okita , M. , Yoshida , S. , Yamamoto , J. , Suzuki , K. , Kaneyuki , T. , Kubota , M. and Sasagawa , T.N‐3 and n‐6 fatty acid intake and serum phospholipid fatty acid composition in middle‐aged women living in rural and urban areas in Okayama Prefecture . J. Nutr. Sci. Vitaminol. , 41 , 313 – 323 ( 1995. ). [DOI] [PubMed] [Google Scholar]
  • 27).Hirai , A. , Terano , T. , Tamura , Y. and Yoshida , S.Eicosapentaenoic acid and adult diseases in Japan: epidemiological and clinical aspects . J. Intern. Med. , 225 ( Suppl. ), 69 – 75 ( 1989. ). [DOI] [PubMed] [Google Scholar]
  • 28).Umemura , U. , Koike , K. A. , Iso , H. , Sankai , T. , Shimamoto , T. , Sato , S. , Iida , M. , Handa , K. and Komachi , Y.Population‐based comparative study on dietary habits and serum fatty acid compositions . Jpn. J. Hyg. , 48 , 939 – 954 ( 1993. ) ( in Japanese ). [DOI] [PubMed] [Google Scholar]
  • 29).Nakamura , T. , Takebe , K. , Tando , Y. , Arai , Y. , Yamada , N. , Ishii , M. , Kikuchi , H. , Machida , K. , Imamura , K. and Terada , A.Serum fatty acid composition in normal Japanese and its relationship with dietary fish and vegetable oil contents and blood lipid levels . Ann. Nutr. Metab. , 39 , 261 – 270 ( 1995. ). [DOI] [PubMed] [Google Scholar]
  • 30).Nikkari , T. , Luukkainen , P. , Pietinen , P. and Puska , P.Fatty acid composition of serum lipid fractions in relation to gender and quality of dietary fat . Ann. Med. , 27 , 491 – 498 ( 1995. ). [DOI] [PubMed] [Google Scholar]
  • 31).Han , K. P. and Hee , Y.Relationship between plasma, erythrocyte membrane, and dietary intake levels of n‐3 fatty acids in young Korean females . Korean J. Nutr. , 28 , 995 – 1003 ( 1995. ). [Google Scholar]
  • 32).Ma , J. , Folsom , A. R. , Eckfeldt , J. H. , Lewis , L. and Chambless , L. E.Short‐ and long‐term repeatability of fatty acid composition of human plasma phospholipids and cholesterol esters. The Atherosclerosis Risk in Communities (ARIC) Study Investigators . Am. J. Clin. Nutr. , 62 , 572 – 578 ( 1995. ). [DOI] [PubMed] [Google Scholar]
  • 33).Hjartaker , A. , Lund , E. and Bjerve , K. S.Serum phospholipid fatty acid composition and habitual intake of marine foods registered by a semi‐quantitative food frequency questionnaire . Eur. J. Clin. Nutr. , 51 , 736 – 742 ( 1997. ). [DOI] [PubMed] [Google Scholar]
  • 34).Karmali , R. A. , Reichel , P. , Cohen , L. A. , Terano , T. , Hirai , A. , Tamura , Y. and Yoshida , S.The effects of dietary omega‐3 fatty acids on the DU‐145 transplantable human prostatic tumor . Anticancer Res. , 7 , 1173 – 1179 ( 1987. ). [PubMed] [Google Scholar]
  • 35).Rose , D. P. and Cohen , L. A.Effects of dietary menhaden oil and retinyl acetate on the growth of DU 145 human pro‐static adenocarcinoma cells transplanted into athymic nude mice . Carcinogenesis , 9 , 603 – 605 ( 1988. ). [DOI] [PubMed] [Google Scholar]
  • 36).Rose , D. P. and Connolly , J. M.Effects of fatty acids and eicosanoid synthesis inhibitors on the growth of two human prostate cancer cell lines . Prostate , 18 , 243 – 254 ( 1991. ). [DOI] [PubMed] [Google Scholar]
  • 37).Harvey , S. , Bjerve , K. S. , Tretli , S. , Jellum , E. , Robsahm , T. E. and Vatten , L.Prediagnostic level of fatty acids in serum phospholipids: omega‐3 and omega‐6 fatty acids and the risk of prostate cancer . Int. J. Cancer , 71 , 545 – 551 ( 1997. ). [DOI] [PubMed] [Google Scholar]
  • 38).Giovannucci , E. , Rimm , E. B. , Colditz , G. A. , Stampfer , M. J. , Ascherio , A. , Chute , C. C. and Willett , W. C.A prospective study of dietary fat and risk of prostate cancer. J . Natl. Cancer Inst. , 85 , 1571 – 1579 ( 1993. ). [DOI] [PubMed] [Google Scholar]
  • 39).Gann , P. H. , Hennekens , C. H. , Sacks , F. M. , Grodstein , F. , Giovannucci , E. L. and Stampfer , M. J.Prospective study of plasma fatty acids and risk of prostate cancer . J. Natl. Cancer Inst. , 86 , 281 – 286 ( 1994. ). [DOI] [PubMed] [Google Scholar]
  • 40).London , S. J. , Sacks , F. M. , Stampfer , M. J. , Henderson , I. C. , Maclure , M. , Tomita , A. , Wood , W. C. , Remine , S. , Robert , N. J. , Dmochowski , J. R. and Willett , W. C.Fatty acid composition of the subcutaneous adipose tissue and risk of proliferative benign breast disease and breast cancer . J. Natl. Cancer Inst. , 85 , 785 – 793 ( 1993. ). [DOI] [PubMed] [Google Scholar]
  • 41).Petrek , J. A. , Hudgins , L. C. , Levine , B. , Ho , M. and Hirsch , J.Breast cancer risk and fatty acids in the breast and abdominal adipose tissues . J. Natl. Cancer Inst. , 86 , 53 – 56 ( 1994. ). [DOI] [PubMed] [Google Scholar]
  • 42).Zhu , Z. R. , Agren , J. , Mannisto , S. , Pietinen , P. , Eskelinen , M. , Syrjanen , K. and Uusitupa , M.Fatty acid composition of breast adipose tissue in breast cancer patients and in patients with benign breast disease . Nutr. Cancer , 24 , 151 – 160 ( 1995. ). [DOI] [PubMed] [Google Scholar]
  • 43).Simonsen , N. , van't Veer , P. , Strain , J. J. , Martin‐Moreno , J. M. , Huttunen , J. K. , Navajas , J. F. , Martin , B. C. , Thamm , M. , Kardinaal , A. F. , Kok , F. J. and Kohlmeier , L.Adipose tissue omega‐3 and omega‐6 fatty acid content and breast cancer in the EURAMIC study. European Community Multicenter Study on Antioxidants, Myocardial Infarction, and Breast Cancer . Am. J. Epidemiol. , 147 , 342 – 352 ( 1998. ). [DOI] [PubMed] [Google Scholar]
  • 44).Willett , W. C.Specific fatty acids and risks of breast and prostate cancer: dietary intake . Am. J. Clin. Nutr. , 66 ( Suppl. ), 1557 – 1563 ( 1997. ). [DOI] [PubMed] [Google Scholar]
  • 45).Klurfeld , D. M. and Bull , A. W.Fatty acids and colon cancer in experimental models . Am. J. Clin. Nutr. , 66 ( Suppl. ), 1530 – 1538 ( 1997. ). [DOI] [PubMed] [Google Scholar]
  • 46).Giovannucci , E. and Goldin , B.The role of fat, fatty acids, and total energy intake in the etiology of human colon cancer . Am. J. Clin. Nutr. , 66 ( Suppl. ), 1564 – 1571 ( 1997. ). [DOI] [PubMed] [Google Scholar]
  • 47).Giovannucci , E. and Willett , W. C.Dietary lipids and colon cancer . Princ. Pract. Oncol. Updates , 9 , 1 – 12 ( 1995. ). [Google Scholar]
  • 48).Franceschi , S. , Favero , A. , la Vecchia , C. , Negri , E. , Conti , E. , Montella , M. , Giacosa , A. , Nanni , O. and Decarli , A.Food groups and risk of colorectal cancer in Italy . Int. J. Cancer , 72 , 56 – 61 ( 1997. ). [DOI] [PubMed] [Google Scholar]
  • 49).Willett , W. C. , Stampfer , M. J. , Colditz , G. A. , Rosner , B. A. and Speizer , F. E.Relation of meat, fat, and fiber intake to the risk of colon cancer in a prospective study among women . N. Engl. J. Med. , 323 , 1664 – 1672 ( 1990. ). [DOI] [PubMed] [Google Scholar]
  • 50).Thun , M. J. , Calle , E. E. , Namboodiri , M. M. , Flanders , W. D. , Coates , R. J. , Byers , T. , Boffetta , P. , Garfinkel , L. and Heath , C. W. , Jr.Risk factors for fatal colon cancer in a large prospective study . J. Natl. Cancer Inst. , 84 , 1491 – 1500 ( 1992. ). [DOI] [PubMed] [Google Scholar]
  • 51).Bostick , R. M. , Potter , J. D. , Kushi , L. H. , Sellers , T. A. , Steinmetz , K. A. , McKenzie , S. M. and Folsom , A. R.Sugar, meat, and fat intake, and non‐dietary risk factors for colon cancer incidence in Iowa women (United States) . Cancer Cause Control , 5 , 38 – 52 ( 1994. ). [DOI] [PubMed] [Google Scholar]
  • 52).Svensson , B. G. , Mikoczy , Z. , Stromberg , U. and Hagmar , L.Mortality and cancer incidence among Swedish fishermen with a high dietary intake of persistent organochlorine compounds . Scand. J. Work Environ. Health , 21 , 106 – 115 ( 1995. ). [DOI] [PubMed] [Google Scholar]
  • 53).Kato , I. , Akhmedkhanov , A. , Koenig , K. , Toniolo , P. G. , Shore , R. E. and Riboli , E.Prospective study of diet and female colorectal cancer: the New York University Women's Health Study . Nutr. Cancer , 28 , 276 – 281 ( 1997. ). [DOI] [PubMed] [Google Scholar]
  • 54).Berg , J. P. , Glattre , E. , Haldorsen , T. , Hostmark , A. T. , Bay , I. G. , Johansen , A. F. and Jellum , E.Longchain serum fatty acids and risk of thyroid cancer: a population‐based case‐control study in Norway . Cancer Causes Control , 5 , 433 – 439 ( 1994. ). [DOI] [PubMed] [Google Scholar]
  • 55).Simon , J. A. , Fong , J. , Bernert , J. T. , Jr. and Browner , W. S.Serum fatty acids and the risk of fatal cancer. MRFIT Research Group. Multiple Risk Factor Intervention Trial . Am. J. Epidemiol. , 148 , 854 – 858 ( 1998. ). [DOI] [PubMed] [Google Scholar]

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