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Japanese Journal of Cancer Research : Gann logoLink to Japanese Journal of Cancer Research : Gann
. 2000 Aug;91(8):769–773. doi: 10.1111/j.1349-7006.2000.tb01011.x

Marked Enhancement by Fish Meal of Helicobacter pylori‐induced Gastritis in Mongolian Gerbils

Tetsuya Tanigawa 1,2, Toshihiko Kawamori 1, Masaki Iimuro 1,2, Toshihisa Ohta 1, Kazuhide Higuchi 2, Tetsuo Arakawa 2, Takashi Sugimura 1, Keiji Wakabayashi 1,✉
PMCID: PMC5926428  PMID: 10965015

Abstract

In a search for dietary factors influencing Helicobacter pylori‐induced gastritis, the effects of fish meal in the diet were examined in Mongolian gerbils. When a conventional diet containing 10% fish meal was given to Mongolian gerbils for 4 weeks after inoculation of H. pylori, edematous thickening with severe neutrophil and mononuclear cell infiltration in both the mucosa and submucosa was observed in the glandular stomach of 19 out of the 20 animals, and hemorrhagic spots were evident in 11 cases. These gastric lesions were enhanced by a 20% fish meal supplement, and edema and hemorrhage in the gastric mucosa were observed in 19 and 17 out of 20 animals, respectively. Although almost the same levels of viable bacteria were detected independent of the diet, edema and hemorrhage were seen in only 2 and 1 of 20 gerbils fed a diet containing 10% casein, instead of 10% fish meal, respectively. Neither edema nor hemorrhage was observed in 10% beef diet animals. These results suggest that fish meal contains factors which greatly enhance H. pylori‐induced gastritis in Mongolian gerbils. Since the incidences of gastritis and gastric cancer are very high throughout the world, it is very important to identify these gastritis‐enhancing factors.

Keywords: H. pylori, Mongolian gerbils, Gastritis, Fish meal

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REFERENCES

  • 1. ) Nomura , A.Stomach cancer . In“Cancer Epidemiology and Prevention ,” ed. Schottenfeld D. and Fraumeni J. F., Jr. , pp. 707 – 724 ( 1996. ). Oxford University Press; , New York . [Google Scholar]
  • 2. ) Forman , D. , Newell , D. G. , Fullerton , F. , Yarnell , J. W. , Stacey , A. R. , Wald , N. and Sitas , F.Association between infection with Helicobacter pylori and risk of gastric cancer: evidence from a prospective investigation . Br. Med. J. , 302 , 1302 – 1305 ( 1991. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 3. ) Parsonnet , J. , Friedman , G. D. , Vandersteen , D. P. , Chang , Y. , Vogelman , J. H. , Orentreich , N. and Sibley , R. K.Helicobacter pylori infection and the risk of gastric carcinoma . N. Engl. J. Med. , 325 , 1127 – 1131 ( 1991. ). [DOI] [PubMed] [Google Scholar]
  • 4. ) Nomura , A. , Stemmermann , G. N. , Chyou , P. H. , Kato , I. , Perez‐Perez , G. I. and Blaser , M. J.Helicobacter pylori infection and gastric carcinoma among Japanese Americans in Hawaii . N. Engl. J. Med. , 325 , 1132 – 1136 ( 1991. ). [DOI] [PubMed] [Google Scholar]
  • 5. ) Hirayama , F. , Takagi , S. , Kusuhara , H. , Iwao , E. , Yokoyama , Y. and Ikeda , Y.Induction of gastric ulcer and intestinal metaplasia in Mongolian gerbils infected with Helicobacter pylori . J. Gastroenterol. , 31 , 755 – 757 ( 1996. ). [DOI] [PubMed] [Google Scholar]
  • 6. ) Sugiyama , A. , Maruta , F. , Ikeno , T. , Ishida , K. , Kawasaki , S. , Katsuyama , T. , Shimizu , N. and Tatematsu , M.Helicobacter pylori infection enhances N‐methyl‐N‐nitrosoureainduced stomach carcinogenesis in the Mongolian gerbil . Cancer Res. , 58 , 2067 – 2069 ( 1998. ). [PubMed] [Google Scholar]
  • 7. ) Honda , S. , Fujioka , T. , Tokieda , M. , Satoh , R. , Nishizono , A. and Nasu , M.Development of Helicobacter pylori‐induced gastric carcinoma in Mongolian gerbils . Cancer Res. , 58 , 4255 – 4259 ( 1998. ). [PubMed] [Google Scholar]
  • 8. ) Shimizu , N. , Inada , K. , Nakanishi , H. , Tsukamoto , T. , Ikehara , Y. , Kaminishi , M. , Kuramoto , S. , Sugiyama , A. , Katsuyama , T. and Tatematsu , M.Helicobacter pylori infection enhances glandular stomach carcinogenesis in Mongolian gerbils treated with chemical carcinogens . Carcinogenesis , 20 , 669 – 676 ( 1999. ). [DOI] [PubMed] [Google Scholar]
  • 9. ) Tokieda , M. , Honda , S. , Fujioka , T. and Nasu , M.Effect of Helicobacter pylori infection on the N‐methyl‐N'‐nitro‐N‐nitrosoguanidine‐induced gastric carcinogenesis in Mongolian gerbils . Carcinogenesis , 20 , 1261 – 1266 ( 1999. ). [DOI] [PubMed] [Google Scholar]
  • 10. ) Shimizu , N. , Ikehara , Y. , Inada , K. , Nakanishi , H. , Tsukamoto , T. , Nozaki , K. , Kaminishi , M. , Kuramoto , S. , Sugiyama , A. , Katsuyama , T. and Tatematsu , M.Eradication diminishes enhancing effects of Helicobacter pylori infection on glandular stomach carcinogenesis in Mongolian gerbils . Cancer Res. , 60 , 1512 – 1514 ( 2000. ). [PubMed] [Google Scholar]
  • 11. ) Watanabe , T. , Tada , M. , Nagai , H. , Sasaki , S. and Nakao , M.Helicobacter pylori infection induces gastric cancer in Mongolian gerbils . Gastroenterology , 115 , 642 – 648 ( 1998. ). [DOI] [PubMed] [Google Scholar]
  • 12. ) Tatematsu , M. , Shimizu , N. and Sugiyama , A.A gastric carcinogenesis model employing Mongolian gerbils infected with Helicobacter pylori— approach to human cancer prevention . 30th Int. Symp . Princess Takamatsu Cancer Research Fund , 52 – 53 ( 1999. ).
  • 13. ) Hirayama , F. , Takagi , S. , Iwao , E. , Yokoyama , Y. , Haga , K. and Hanada , S.Development of poorly differentiated adenocarcinoma and carcinoid due to long‐term Helicobacter pylori colonization in Mongolian gerbils . J. Gastroenterol. , 34 , 450 – 454 ( 1999. ). [DOI] [PubMed] [Google Scholar]
  • 14. ) Blaser , M. J.Hypothesis: the changing relationships of Helicobacter pylori and humans: implications for health and disease . J. Infect. Dis. , 179 , 1523 – 1530 ( 1999. ). [DOI] [PubMed] [Google Scholar]
  • 15. ) IARC Monographs on the Evaluation of Carcinogenic Risks to Humans; Schistosomes, Liver Flukes and Helicobacter pylori . Vol. 61 , pp. 177 – 240 ( 1994. ). IARC; , Lyon . [PMC free article] [PubMed] [Google Scholar]
  • 16. ) Nomura , A. , Yamakawa , H. , Ishidate , T. , Kamiyama , S. , Masuda , H. , Stemmermann , G. N. , Heilburn , L. K. and Hankin , J. H.Intestinal metaplasia in Japan: association with diet . J. Natl. Cancer Inst. , 68 , 401 – 405 ( 1982. ). [PubMed] [Google Scholar]
  • 17. ) Ikeda , M. , Yoshimoto , K. , Yoshimura , T. , Kono , S. , Kato , H. and Kuratsune , M.A cohort study on the possible association between broiled fish intake and cancer . Gann , 74 , 640 – 648 ( 1983. ). [PubMed] [Google Scholar]
  • 18. ) Kato , I. , Tominaga , S. , Ito , Y. , Kobayashi , S. , Yoshii , Y. , Matsuura , A. , Kameya , A. and Kano , T.A comparative case‐control analysis of stomach cancer and atrophic gastritis . Cancer Res. , 50 , 6559 – 6564 ( 1990. ). [PubMed] [Google Scholar]
  • 19. ) al‐Harbi , M. M. , Islam , M. W. , al‐Shabanah , O. A. and al‐Gharably , N. M.Effect of acute administration of fish oil (omega‐3 marine triglyceride) on gastric ulceration and secretion induced by various ulcerogenic and necrotizing agents in rats . Food Chem. Toxicol. , 33 , 553 – 558 ( 1995. ). [DOI] [PubMed] [Google Scholar]
  • 20. ) Faust , T. , Redfern , J. S. , Lee , E. and Feldman , M.Effects of fish oil on gastric mucosal 6‐keto‐PGFl alpha synthesis and ethanol‐induced injury . Am. J. Physiol. , 257 , G9 – 13 ( 1989. ). [DOI] [PubMed] [Google Scholar]
  • 21. ) Polavarapu , R. , Spitz , D. R. , Sim , J. E. , Follansbee , M. H. , Oberley , L. W. , Rahemtulla , A. and Nanji , A. A.Increased lipid peroxidation and impaired antioxidant enzyme function is associated with pathological liver injury in experimental alcoholic liver disease in rats fed diets high in corn oil and fish oil . Hepatology , 27 , 1317 – 1323 ( 1998. ). [DOI] [PubMed] [Google Scholar]

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