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Japanese Journal of Cancer Research : Gann logoLink to Japanese Journal of Cancer Research : Gann
. 2001 Mar;92(3):321–327. doi: 10.1111/j.1349-7006.2001.tb01098.x

Efficient Gene Transduction by RGB‐fiber Modified Recombinant Adenovirus into Dendritic Cells

Rumiko Asada‐Mikami 1,4, Yuji Heike 5,✉, Sachiyo Kanai 1, Masato Azuma 1, Kazuo Shirakawa 1, Yoichi Takaue 3, Victor Krasnykh 6, David T Curiel 6, Masaaki Terada 2, Tatsuo Abe 4, Hiro Wakasugi 1,✉
PMCID: PMC5926705  PMID: 11267943

Abstract

Dendritic cells (DC) are important antigen‐presenting cells in the development of an anti‐tumor T cell response. To extend the range of current immuno/gene therapies, we tested luciferase‐expressing RGD‐adenovirus (Ad) (Ad5lucRGD)‐mediated transduction into DC. Phenotypically characterized DC were generated from peripheral blood CD14+ cells by incubation with granulocyte‐macrophage colony‐stimulating factor, interleukin‐4 and tumor necrosis factor a. On the 7th day of culture, the cells became mature DC with a CD1a+, CD11c+, CD80+, CD83+, CD86+, human leukocyte antigen (HLA)‐DR+, CD14− phenotype. The expression of (α,β3 integrin was enhanced on day 3 and returned to the basal level on day 7. We then compared the transduction efficiency of an AdSlucRGD system to that using conventional Ad, in cells harvested on days 1, 3 and 7 of culture. Luciferase activity was negligible in AdCMVLuc, but remarkable in cells processed with Ad5lucRGD. Activity was maximal in cells that had been cultured for 3 days. Recombinant Ad5 fiber knob protein blocked AdCMVLuc‐ and Ad5lucRGD‐mediated gene transduction by 90% and 20%, respectively. Surface markers and cytokine production were not affected by Ad5lucRGD‐mediated transduction.

Keywords: Adenoviridae, Gene tranfer, Dendritic cell, Integrins, Coxsackie, adenovirus

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