Abstract
Associations between Helicobacter pylori (HP) infection and lifestyle factors have been reported by several authors, but little is known about the host factors associated with the infection. This study aims to examine the infection rate of HP according to gene polymorphisms of interleukin (TL)‐IA, IL‐1B, and IL‐1RN, and to investigate the interactions with lifestyle factors. Subjects were 241 non‐cancer outpatients who had participated in a HP eradication program. Polymorphisms at ‐889 (T to C) of IL‐1A, at ‐31 (C to T; T allele makes a TATA box) and ‐511 (C to T) of IL‐1B, and at intron 2 (86‐bp VNTR (variable number of tandem repeats)) of IL‐1RN were genotyped by PCR (polymerase chain reaction), PCR‐RFLP (restriction fragment length polymorphism) and PCR‐CTPP (PCR with confronting two‐pair primers). It was found that IL‐1B polymorphisms at ‐31 and ‐511 were near‐completely linked, but in the opposite way to that in Caucasians; ‐31C/ ‐511T and ‐31T/‐511C alleles were dominant in the present subjects. The HP infection rate was substantially different among the genotypes of IL‐1B C‐31T; 45.2% (19/42) for the C/C, 67.7% (90/133) for the C/T, and 63.6% (42/66) for the T/T. The age‐sex adjusted odds ratio (OR) relative to the C/C genotype was 2.32 (95%CI (confidence interval), 1.10‐4.92) for the T/C genotype and 2.46 (1.06‐5.74) for the T/T genotype. The OR for the T/T genotype was significantly modified by smoking status; interaction term=14.6 (1.12‐190). The polymorphisms of IL‐1A and IL‐1RN were not associated with the infection rate. The results suggested that the T allele of IL‐1B C‐31T is associated with vulnerability to persistent HP infection, and that the vulnerability is modified by smoking.
Keywords: Helicobacter pylori infection, Interleukin 1, Lifestyle factors
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References
- 1.Banatvala , N. , Mayo , K. , Megraud , F. , Jennings , R. , Deeks , J. J . and Feldman , R. A . The cohort effect and Helico‐bacterpylori . J. Infect. Dis. , 168 , 219 – 221 ( 1993. ). [DOI] [PubMed] [Google Scholar]
- 2.Goodman , K. J . and Correa , P . The transmission of Helico‐bacter pylori. A critical review of the evidence . Int. J. Epi-demiol. , 24 , 875 – 887 ( 1995. ). [DOI] [PubMed] [Google Scholar]
- 3.Cave , D. R . How is Helicobacter pylori transmitted ? Gas-troenterology , 113 , S9 – S14 ( 1997. ). [DOI] [PubMed] [Google Scholar]
- 4.El‐Omar , E. M. , Carrington , M. , Chow , W.‐H. , McColl , K. E. L. , Bream , J. H. , Young , H. A. , Herrera , J. , Lissowska , J. , Yuan , C.‐C. , Rothman , N. , Lanyon , G. , Martin , M. , Fraumeni , J. F. , Jr. . and Rabkin , C. S . Interleukin‐1 polymorphisms associated with increased risk of gastric cancer . Nature , 404 , 398 – 402 ( 2000. ). [DOI] [PubMed] [Google Scholar]
- 5.Dianarello , C. A . Biologic basis for interleukin‐1 in disease . Blood , 87 , 2095 – 2147 ( 1996. ). [PubMed] [Google Scholar]
- 6.Jung , H. , Kim , J. M. , Song , I. S . and Kim , C. Y . Helicobacter pylori induces an array of pro‐inflammatory cyto‐kines in human gastric epithelial cells: quantification of mRNA for interleukin‐8, ‐1 alpha/beta, granulocyte‐mac‐rophage colony‐stimulating factor, monocyte chemoattrac‐tant protein‐1 and tumor necrosis factor‐alpha . J. Gastroenterol. Hepatol. , 12 , 473 – 480 ( 1997. ). [DOI] [PubMed] [Google Scholar]
- 7.Santtilla , S. , Savinainen , K . and Hurme , M . Presence of the IL‐1RA allele 2 (IL1RN*2) is associated with enhanced IL‐Ibeta production in vitro . Scand. J. Immunol. , 47 , 195 – 198 ( 1998. ). [DOI] [PubMed] [Google Scholar]
- 8.Hulkkonen , J. , Laippala , P . and Hurme , M . A rare allele combination of the interluekin‐1 gene complex is associated with high interleukin‐lβ plasma levels in healthy individuals . Eur. Cytokine Netw. , 11 , 251 – 255 ( 2000. ). [PubMed] [Google Scholar]
- 9.Evans , D. J. , Evans , D. G. , Graham , D. Y . and Klein , P. D . A sensitive and specific serologic test for detection of Campylobacter pylori infection . Gastroenterology , 1004 – 1008 ( 1989. ). [DOI] [PubMed] [Google Scholar]
- 10.Matsuo , K. , Hamajima , N. , Tominaga , S. , Suzuki , T. , Nakamura , T. , Matsuura , A . and Kitayama , K . Helicobacter pylori IgG antibody test established in the United States showed a substantial lower sensitivity for Japanese population . Am. J. Gastroenterol. , 95 , 1597 – 1598 ( 2000. ). [DOI] [PubMed] [Google Scholar]
- 11.Mansfield , J. C. , Holden , H. , Tarlow , J. K. , DiGiovine , F. S. , McDowell , T. J. , Wilson , A. G. , Holdsworth , C. D . and Duff , G. W . Novel genetic association between ulcerative colitis and the anti‐inflammatory cytokine interleukin‐1 receptor antagonist . Gastroenterology , 106 , 637 – 642 ( 1994. ). [DOI] [PubMed] [Google Scholar]
- 12.Hamajima , N. , Saito , T. , Matsuo , K. , Kozaki , K. , Takahashi , T . and Tajima , K . Polymerase chain reaction with confronting two‐pair primers for polymorphism geno‐typing . Jpn. J. Cancer Res. , 91 , 865 – 868 ( 2000. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 13.The EUROGAST Study Group. Epidemiology of, and risk factors for Helicobacter pylori infection among 2194 asymptomatic subjects in 17 populations . Gut , 34 , 1672 – 1676 ( 1993. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 14.Fukao , A. , Komatsu , S. , Tsubono , Y. , Hisamichi , S. , Ohori , H. , Kizawa , T. , Ohsato , N. , Fujino , N. , Endo , N . and Iha , M . Helicobacter pylori infection and chronic atrophic gastritis among Japanese blood donors: a cross‐sectional study . Cancer Causes Control , 4 , 307 – 312 ( 1993. ). [DOI] [PubMed] [Google Scholar]
- 15.Tsugane , S. , Tei , Y. , Takahashi , T. , Watanabe , T . and Sugano , K . Salty food intake and risk of Helicobacter pylori infection . Jpn. J. Cancer Res. , 85 , 474 – 478 ( 1994. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 16.Asaka , M. , Takeda , H. , Sugiyama , T . and Kato , M . What role does Helicobacter pylori play in gastric cancer ? Gas-troenterology , 113 , S56 – S60 ( 1997. ). [DOI] [PubMed] [Google Scholar]
- 17.Bailly , S. , di Giovine , F. S. , Blakemore , A. I. F . and Duff , G. W . Genetic polymorphism of human interleukin‐1α . Eur. J. ImmunoL , 23 , 1240 – 1245 ( 1993. ). [DOI] [PubMed] [Google Scholar]
- 18.Bergholdt , R. , Karlsen , A. E. , Johannesen , J. , Hansen , P. M. , Dinarello , C. A. , Nerup , J. , Pociot , F . and The Danish Study Group of Diabetes in Childhood. Characterization of polymorphisms of an interleukin 1 receptor type 1 gene (IL1RI) promoter region (P2) and their relation to insulin‐dependent diabetes mellitus (IDDM) . Cytokine , 1 , 727 – 733 ( 1995. ). [DOI] [PubMed] [Google Scholar]
- 19.Kuipers , E. J. , Lundell , L. , Klinkenberg‐Knol , E. C. , Havu , N. , Festen , H. P. M. , Kiedman , B. , Lamers , C. B. H. W. , Jansen , J. B. M. J . and Dalenback , J . Atrophic gastritis and Helicobacter pylori infection in patients with reflux esoph‐agitis treated with omeprazole or fundoplication . N. Engl. J. Med. , 334 , 1018 – 1022 ( 1996. ). [DOI] [PubMed] [Google Scholar]
- 20.Beales , I. L . and Calam , J . Interleukin 1 beta and tumour necrosis factor alpha inhibit acid secretion in cultured rabbit parietal cells by multiple pathways . Gut , 42 , 227 – 234 ( 1998. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 21.Murray , L. J. , McCrum , E. E. , Evans , A. E. and Bamford , K. B . Epidemiology of Helicobacter pylori infection among 4742 randomly selected subjects from Northern Ireland . Int. J. Epidemiol. , 26 , 880 – 887 ( 1997. ). [DOI] [PubMed] [Google Scholar]
- 22.Woodward , M. , Morrison , C . and McColl , K . An investigation into factors associated with Helicobacter pylori infection . J. Clin. Epidemiol. , 53 , 175 – 182 ( 2000. ). [DOI] [PubMed] [Google Scholar]
- 23.Hamajima , N. , Inoue , M. , Tajima , K. , Tominaga , S. , Matsuura , A. , Kobayashi , S . and Ariyoshi , Y . Lifestyle and anti‐Helicobacter pylori immunoglobulin G antibody among outpatients . Jpn. J. Cancer. Res. , 88 , 1038 – 1043 ( 1997. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 24.Fontham , E. T. H. , Ruiz , B. , Perez , A. , Hunter , F . and Correa , P . Determinations of Helicobacter pylori infection and chronic gastritis . Am. J. Gastroenterol. , 90 , 1094 – 1101 ( 1995. ). [PubMed] [Google Scholar]
- 25.Bertoni , G. , Sassatelli , R. , Nigrisoli , E. , Tansini , P. , Bianchi , G. , Casa , G. D. , Gagni , A . and Bedogni , G . Triple therapy with azithromycin, omeprazole, and amoxicillin is highly effective in the eradication of Helicobacter pylori: a controlled trial versus omeprazole plus amoxicillin . Am. J. Gastroenterol. , 91 , 258 – 263 ( 1996. ). [PubMed] [Google Scholar]