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. 2001 Aug;92(8):854–862. doi: 10.1111/j.1349-7006.2001.tb01172.x

Frequent Increase of DNA Copy Number in the 2q24 Chromosomal Region and Its Association with a Poor Clinical Outcome in Hepatoblastoma: Cytogenetic and Comparative Genomic Hybridization Analysis

Kazuko Kumon 1, Hirofumi Kobayashi 1, Takeshi Namiki 1, Yukiko Tsunematsu 2, Jun Miyauchi 2, Atsushi Kikuta 3, Yasuo Horikoshi 4, Yoshihiro Komada 5, Yoshiro Hatae 6, Haruhiko Eguchi 7, Yasuhiko Kaneko 1,✉
PMCID: PMC5926834  PMID: 11509117

Abstract

In a cytogenetic and comparative genomic hybridization (CGH) study of 38 hepatoblastomas, we found gain of Iq in 17 tumors (44.7%), that of 2/2q in 14 (36.8%), that of 20/20q in 9 (23.7%) and that of 8/8q in 8 (21.0%), loss of 4q in 4 (10.5%) and no DNA copy changes with normal karyotype or no mitotic cells in 11 (28.9%). Eleven tumors with 2/2q gain detected by CGH had a total chromosome 2 gain, a partial 2q gain, or a total chromosome 2 gain with an augmented partial 2q region; the common region for DNA copy gain was 2q24. Two‐color fluorescence in situ hybridization (FISH) analyses using probes covering the centromere of chromosome 2 or HOXD13 (2q31) confirmed the CGH findings, and showed that the common region for gain in 2q was centromeric to HOXD13. Event‐free survival (EFS)±standard error (SE) at 5 years was lowest in patients with 2q gain [37±15%], highest in those with no DNA copy changes [82±12%], and intermediate in those with DNA copy changes other than 2q gain [74±13%] (P=0.0549). Multivariate analysis showed that 2q gain was an independent factor predicting a poor outcome. These findings suggest the presence of a growth‐promoting gene or an oncogene in the 2q24 chromosome band, and a tumor suppressor gene in terminal 4q, which have important roles in the development and progression of hepatoblastoma.

Keywords: Hepatoblastoma, CGH, FISH, Chromosome abnormalities, 2q gain

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