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Japanese Journal of Cancer Research : Gann logoLink to Japanese Journal of Cancer Research : Gann
. 2002 Mar;93(3):284–290. doi: 10.1111/j.1349-7006.2002.tb02170.x

Multiplexed Analysis of Post‐PCR Fluorescence‐labeled Microsatellite Alleles and Statistical Evaluation of Their Imbalance in Brain Tumors

Koji Yoshimoto 1, Toru Iwaki 2, Takanori Inamura 3, Masashi Fukui 3, Tomoko Tahira 1, Kenshi Hayashi 1,✉
PMCID: PMC5926965  PMID: 11927010

Abstract

Detection of the loss of chromosomal regions in cancerous tissues has diagnostic and prognostic relevance, and the development of a reliable and cost‐effective technique for this is clinically important. Here we present an efficient technique for quantitative detection of microsatellite alleles, using a post‐PCR fluorescence‐labeling procedure and multiplexed analysis. We also present a new statistical method for the interpretation of the data that permits reliable and sensitive evaluation of the allelic status of sampled DNA. A high‐resolution analysis of allelic imbalance on chromosomes 1p, 10 and 19q in 28 glioma samples of various types using this method revealed that allelic imbalances are more frequent than have been reported, suggesting the diagnostic value of this method in examining the genetic profiles of gliomas.

Keywords: Glioma, Chromosome 10, Statistical assessment, Allelic imbalance, Post‐PCR fluorescence‐labeling

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