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. 2002 Apr;93(4):397–403. doi: 10.1111/j.1349-7006.2002.tb01270.x

Expansion of Lung Vα14 NKT Cells by Administration of α‐Galactosylceramide‐pulsed Dendritic Cells

Yasunori Akutsu 1,3,5, Toshinori Nakayama 1,3,4, Michishige Harada 1,3, Tetsu Kawano 1,3, Shinichiro Motohashi 1,3, Eiko Shimizu 1,3, Toshihiro Ito 1,3, Noriaki Kamada 1,3, Takeshi Saito 1,3,5, Hisahiro Matsubara 5, Yukimasa Miyazawa 5, Takenori Ochiai 5, Masaru Taniguchi 1,2,3,✉
PMCID: PMC5927009  PMID: 11985789

Abstract

NKT cells, a novel murine lymphoid lineage bearing an invariant T cell receptor encoded by Vα14 and Jα281 gene segments, recognize a specific ligand glycolipid, α‐galactosylceramide (α‐GalCer) in a CDld‐dependent manner. Recent research has revealed that activated Vα14 NKT cells have dramatic antitumor effects against a wide variety of tumor cell lines in vivo and in vitro. Here, we demonstrate strong in vivo antitumor effects brought about by treatment with α‐GalCer‐pulsed dendritic cells in comparison with in vitro‐activated Vα14 NKT cells. Furthermore, we show a significant expansion of endogenous Vα14 NKT cells in the lung following the administration of α‐GalCer‐pulsed dendritic cells. The feasibility of immunotherapy with α‐GalCer‐pulsed dendritic cells is discussed.

Keywords: NKT cell, Dendritic cell, α‐Galactosylceramide

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