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. 2018 Mar 8;103(5):787–798. doi: 10.3324/haematol.2017.180992

Figure 4.

Figure 4.

Hemin, red blood cell (RBC) count and hypoxia treatment increase expression of matriptase MT-SP1 in human THP1-derived macrophages. (A and B) Meta-analysis of membrane type serine protease 1 (MT- SP1) expression in human primary monocyte-derived macrophages using Geo database NCBI (A) Data Set GDS3203 and (B) Data Set GDS2036. For quantification graphs, mean and Standard Deviation (SD) are shown. (C and E) Western blot of MT- SP1 in human THP-1-derived macrophages treated with different concentrations of hemin (C) or RBC lysate (D). β-actin was used for normalization. (D and F) Quantification of MT-SP1 on Western blot. For quantification graphs, mean and SD are shown. *P<0.05. Results are representative of three independent experiments. (G) Immunostaining of MT- SP1 in THP-1-derived macrophages treated with hemin (10 nM) or RBC lysate (green). DAPI was used for nuclear staining. Bar size 40 μm. PMA: phorbol 12-myristate 13-acetate.