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. 2018 Feb 12;37(18):2394–2409. doi: 10.1038/s41388-017-0119-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, which permits any non-commercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. If you remix, transform, or build upon this article or a part thereof, you must distribute your contributions under the same license as the original. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/.

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Fig. 1 — E6 and E7 regulate the expression of splicing factors in CC cells. a Summary of microarray analyses. In all, microarray analyses were performed in Caski and Ms751 cells transiently transfected with either siE6E7 or control siRNA to screen the differentially expressed genes. A total of 1535 genes were identified after overlap in Caski and Ms751 cells. b Top significant pathways for overlapped differentially expressed genes in E6E7-knockdown cells analyzed by KEGG pathway enrichment analysis. Spliceosome ranked fourth. c Top significant pathways for overlapped differentially expressed genes in E6E7-knockdown cells analyzed by DAVID enrichment analysis. Alternative splicing is the second pathway affected by E6E7 knockdown. d The heatmap of differentially expressed genes in AS pathway in Caski and Ms751 groups. e The schematic diagram of differentially expressed core splicing factors. In all, 19 genes were identified by overlapping differentially expressed genes related to AS pathway in microarray and core splicing factors in Ref. [16]. f Validation of the microarray results by RT-PCR of the 19 core splicing factors in Ms751 and SiHa cells. The expression of HNRPDL is undetectable. The experiments were repeated three times, and the results are shown as mean ± s.d. *P < 0.05; **P < 0.01; ***P < 0.001 (Student’s t-test)