Key resources table.
Reagent type (species) or resource |
Designation | Source or reference | Identifiers |
---|---|---|---|
Strain, strain background (M. musculus) | C57BL/6J mice | Charles River | Strain code 027 |
Strain, strain background (M. musculus) | DAT-Cre, B6.SJL-Slc6a3tm1.1(cre)Bkmn/J | The Jackson Laboratory | Jax stock 006660 |
Strain, strain background (M. musculus) | VGAT-Cre, Slc32a1tm2(cre)Lowl/J | The Jackson Laboratory | Jax stock 016962 |
Strain, strain background (M. musculus) | flex-tdTomato, B6.Cg–Gt(ROSA)26Sortm9(CAG-tdTomato)Hze, | The Jackson Laboratory | Jax stock 007909 |
Strain, strain background (M. musculus) | flex-GCaMP6s animals, Ai96; B6;129S6-Gt(ROSA)26Sortm96(CAG-GCaMP6s)Hze/J | The Jackson Laboratory | Jax stock 024106 |
Transfected construct (Adeno-associated virus) | AAV9.EF1a.ChR2-YFP lox/lox virus | Penn Vector Core, USA | AV-9-PV1522, |
Transfected construct (retrovirus) | floxed rv::dio-GFPlox/lox | Oscar Marin | Ciceri et al., 2013 Nat Neuroscience 16(9):1199–210 |
Antibody | polyclonal Anti-Tyrosine Hydroxylase, raised in Rabbit; use 1:500 | Millipore | catalogue number AB152; RRID:AB_390204 |
Antibody | monoclonal Anti-Tyrosine Hydroxylase, raised in mouse; use 1:500 | Millipore | clone (LNC1) - catalog number MAB318; RRID: AB_2313764 |
Antibody | polyclonal Anti-Tyrosine Hydroxylase, raised in chicken; use 1:250 | Abcam | catalog number ab76442; RRID:AB_1524535 |
Antibody | monoclonal anti-Ankyrin-G IgG2a, raised in mouse; use 1:500 | Neuromab | clone (106/36) - catalog number75–146; RRID: AB_10673030 |
Antibody | monoclonal anti-Ankyrin-G IgG2b, raised in mouse; use 1:500 | Neuromab | clone (106/65) - catalog number75–147; RRID: AB_10675130 |
Antibody | monoclonal anti-Ankyrin-G IgG1, raised in mouse; use 1:500 | Neuromab | clone (106/20) - catalog number75–187; RRID:AB_10674433 |
Antibody | monoclonal Phospho-IκBα (Ser32) (14D4), raised in rabbit; use 1:1000 | Cell Signaling Technology | catalog number 2859; RRID:AB_561111 |
Antibody | polyclonal Anti-TRIM46, raised in rabbit; use 1:500 | Gift from Casper Hoogenraad | van Beuningen et al. (2015) Neuron. 88:1208–1226 |
Antibody | monoclonal anti-MAP-2, raised in mouse; use 1:500 | Gift from Phillip Gordon-Weeks | |
Antibody | monoclonal anti-chemical BrdU, raised in Rat; use 1:200 | Serotec | clone BU1/75 (ICR1)- catalog number OBT0030; RRID:AB_609568 |
Antibody | polyclonal anti-GFP, raised in chicken; use 1:2000 | Abcam | catalog number ab13970; RRID:AB_300798 |
Antibody | polyclonal anti-GFP, raised in guinea pig; use 1:500 | Synaptic Systems | catalog 132 005; RRID:AB_11042617 |
Chemical compound, drug | Heparin | Alfa Aesar | CAS A16198 |
C | |||
Chemical compound, drug | PIPES | Sigma | CAS P6757 |
Chemical compound, drug | 5-Bromo-2′-deoxyuridine | Sigma | CAS 59143 |
Chemical compound, drug | Alexa 488 | Thermo Fisher Scientific | A10436 |
Chemical compound, drug | Methyl Propionate | Sigma | CAS 81988 |
Chemical compound, drug | Methyl Butyrate | Sigma | CAS 246093 |
Chemical compound, drug | Ethyl Valerate | Sigma | CAS 290866 |
Chemical compound, drug | Hexanal | Sigma | CAS 115606 |
Chemical compound, drug | Methyl Tiglate | Penta | CAS 13–73400 |
Chemical compound, drug | Valeraldehyde | Sigma | CAS 110132 |
Chemical compound, drug | Propyl Acetate | Tokyo Chemical Industry | CAS A0044 |
Chemical compound, drug | Pentyl Acetate | Sigma | CAS 109549 |
Chemical compound, drug | Diethyl Phthalate | Sigma | CAS 84662 |
Software, algorithm | ImageJ software (Fiji) | NIH; Schneider et al. (2012) | RRID:SCR_003070 |
Software, algorithm | ClampFit 10.4 | pClamp | Molecular Devices; RRID:SCR_011323 |
Software, algorithm | Prism 5.3 | GraphPad | RRID:SCR_002798 |
Software, algorithm | Matlab | Mathworks | RRID:SCR_001622 |
Software, algorithm | Vaa3D | Allen Institute for Brain Science | RRID:SCR_002609 |
Software, algorithm | IBM SPSS Statistics | IBM | RRID:SCR_002865 |