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. Author manuscript; available in PMC: 2019 May 3.
Published in final edited form as: Cell. 2018 Mar 22;173(4):851–863.e16. doi: 10.1016/j.cell.2018.03.010

Figure 7. Protecting Mouse Kidneys with BAM15/PI during Cold Storage.

Figure 7

Immunofluorescence of α-tubulins (TUBA; green) and DAPI (magenta) in transverse sections of wild-type mouse kidneys under these conditions (n = 4–6 animals used for each condition): freshly fixed, after 24-h cold storage at 4°C (with or without BAM15/PI) or rewarming at 37°C (with or without BAM15/PI) for 30 min following 24-h cold storage. Note: Reduced tubulin signals in glomeruli (arrowheads in upper panel; middle panel) and renal tubular epithelium (lower panel) of mouse kidneys stored at 4°C for 24 h in standard University of Wisconsin (UW) Solution. Scale bars: 25 μm in all panels. See also Figure S7 for oxidative damages and cell apoptosis in mouse kidneys during cold storage.