Figure 4. Effects of EcTI on integrin expression and intracellular signalling.

Integrin β₁ protein expression: EcTI affects integrin β₁ protein expression in GBM U87 cells (A), MSCs (B), and coculture (C). The cells were treated with EcTI (50 and 100 μM) for 24 h. After SDS-PAGE separation of cell lysates and electrotransfer to PVDF membrane, the binding with anti-integrin β₁ and anti-β-actin (as loading control) was performed and detected by chemiluminescence and relative to the integrin β₁ expression quantitation that was performed by densitometry analysis in the Image J software with β-actin normalization. The percentage of protein expression in treated cells is presented relative to its expression in non-treated control (c) cells. Kinase signalling: The expressions of the ratio p-FAK/FAK in U87 (D); MSC (E); and coculture (F); p-Src/Src in U87 (G); MSC (H); and coculture (I) were analyzed, as described in Material and Methods. All the proteins were normalized by β-actin. Error bars represent SD (^*p < 0.05, ^**p< 0.005, ^***p< 0.0005).