Table 6.
Ellagitannin metabolite profile in the cecal digesta and in the urine of rats fed experimental diets *.
| Cecum | Urine | ||||
|---|---|---|---|---|---|
| Nasutin A 1 | Released EA | Nasutin A glucuronide 2 | Nasutin A 1 | Isonasutin A 3 | |
| µg/g | mg/g | µg/mL | µg/mL | µg/mL | |
| Group (n = 8) | |||||
| C + ME | 43.1 a | 1.42 | 1305 a | 76.5 a | 83.2 |
| F + ME | 30.6 b | 1.52 | 824 b | 60.4 a,b | 82.6 |
| C + DE | 10.4 c | 1.51 | 360 c | 41.8 b | 12.5 |
| F + DE | 10.6 c | 1.47 | 369 c | 68.0 a | 19.5 |
| SEM | 2.905 | 0.039 | 94.51 | 5.100 | 7.678 |
| Extract (E) | |||||
| ME | 36.8 a | 1.47 | 1064 a | 68.5 | 82.9 a |
| DE | 10.5 b | 1.49 | 365 b | 54.9 | 16.0 b |
| p value | <0.001 | NS | <0.001 | NS | <0.001 |
| Diet (D) | |||||
| Starch | 26.8 | 1.47 | 832 | 59.2 | 47.8 |
| Fructose | 20.6 | 1.50 | 597 | 64.2 | 51.0 |
| p value | NS | NS | NS | NS | NS |
| Interaction E × D | |||||
| p value | 0.048 | NS | 0.047 | 0.036 | NS |
* C + ME, fed a corn starch diet with a monomeric ET-rich extract; F + ME, fed a fructose diet with a monomeric ET-rich extract; C + DE, fed a corn starch diet with a dimeric ET-rich extract; F + DE, fed a fructose diet with a dimeric ET-rich extract. a,b,c Mean values within a column with different superscript letters were shown to be significantly different (p < 0.05); differences among the groups (C + ME, F + ME, C + DE, and F + DE) are indicated with superscripts only in the case of a statistically significant interaction E × D (p < 0.05). 1 HPLC retention time (min) 10.08; MS [M − H]− 269; MS/MS fragments 113, 85.03; UV spectra (nm) 227, 245, 284, 323, 389. 2 HPLC retention time (min) 6.99; MS [M − H]− 445; MS/MS fragments 269.01, 113, 85.03; UV spectra (nm) 222, 279, 315, 367, 379. 3 HPLC retention time (min) 10.21; MS [M − H]− 269; MS/MS fragments 113, 85.03; UV spectra (nm) 227, 245, 284, 323, 389.