Table 7.
Correlation analyses between protein structure spectral characteristics and chemical profile of canola seeds.
| Items | ** AI_II_T | AI | AII | R_AAI_II | AIH | AIIH | R_HAI_II | Alpha | Beta | R_Ha_b | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| *** r Value | p Value | R Value | p Value | r Value | p Value | R Value | p Value | r Value | p Value | R Value | p Value | r Value | p Value | r Value | p Value | r Value | p Value | r Value | p Value | |
| * DM (%) | −0.45 | 0.012 | −0.33 | 0.072 | −0.07 | 0.704 | −0.28 | 0.133 | −0.22 | 0.251 | 0.18 | 0.356 | −0.54 | 0.002 | −0.19 | 0.328 | −0.29 | 0.116 | 0.22 | 0.235 |
| Ash (%DM) | −0.10 | 0.614 | −0.03 | 0.866 | 0.02 | 0.913 | 0.00 | 0.986 | 0.01 | 0.973 | 0.04 | 0.821 | 0.02 | 0.939 | −0.01 | 0.965 | 0.09 | 0.621 | −0.17 | 0.368 |
| EE (%DM) | 0.22 | 0.242 | 0.16 | 0.402 | −0.13 | 0.496 | 0.29 | 0.122 | 0.09 | 0.624 | −0.11 | 0.561 | 0.26 | 0.161 | 0.05 | 0.786 | 0.06 | 0.748 | −0.11 | 0.578 |
| FA (%DM) | 0.22 | 0.242 | 0.16 | 0.402 | −0.13 | 0.496 | 0.29 | 0.122 | 0.09 | 0.624 | −0.11 | 0.561 | 0.26 | 0.161 | 0.05 | 0.786 | 0.06 | 0.748 | −0.11 | 0.578 |
| Protein profile | ||||||||||||||||||||
| CP (%DM) | 0.08 | 0.659 | 0.18 | 0.337 | 0.12 | 0.518 | 0.00 | 0.985 | 0.25 | 0.188 | 0.36 | 0.053 | −0.16 | 0.402 | 0.23 | 0.219 | 0.29 | 0.120 | −0.08 | 0.682 |
| SCP (%DM) | 0.52 | 0.003 | 0.39 | 0.033 | 0.20 | 0.282 | 0.09 | 0.642 | 0.27 | 0.151 | 0.04 | 0.840 | 0.23 | 0.225 | 0.26 | 0.160 | 0.20 | 0.279 | 0.09 | 0.656 |
| SCP (%CP) | 0.52 | 0.003 | 0.37 | 0.042 | 0.18 | 0.346 | 0.12 | 0.539 | 0.23 | 0.215 | −0.03 | 0.890 | 0.28 | 0.130 | 0.24 | 0.211 | 0.18 | 0.347 | 0.07 | 0.716 |
| NPN (%DM) | −0.30 | 0.106 | −0.15 | 0.433 | −0.21 | 0.277 | 0.07 | 0.722 | 0.00 | 0.996 | 0.08 | 0.684 | −0.11 | 0.582 | −0.05 | 0.803 | 0.03 | 0.867 | −0.26 | 0.173 |
| NPN (%CP) | −0.32 | 0.081 | −0.18 | 0.347 | −0.23 | 0.217 | 0.08 | 0.673 | −0.03 | 0.860 | 0.02 | 0.914 | −0.09 | 0.646 | −0.09 | 0.657 | −0.01 | 0.954 | −0.24 | 0.202 |
| NPN (%SCP) | −0.41 | 0.025 | −0.25 | 0.181 | −0.16 | 0.394 | −0.06 | 0.740 | −0.09 | 0.637 | 0.13 | 0.502 | −0.28 | 0.140 | −0.11 | 0.572 | −0.07 | 0.723 | −0.11 | 0.549 |
| NDICP (%DM) | −0.52 | 0.003 | −0.32 | 0.090 | −0.16 | 0.400 | −0.08 | 0.686 | −0.15 | 0.425 | 0.07 | 0.722 | −0.26 | 0.170 | −0.16 | 0.413 | −0.09 | 0.634 | −0.12 | 0.528 |
| NDICP (%CP) | −0.57 | 0.001 | −0.39 | 0.036 | −0.18 | 0.335 | −0.11 | 0.579 | −0.22 | 0.235 | −0.01 | 0.980 | −0.25 | 0.183 | −0.22 | 0.253 | −0.16 | 0.408 | −0.12 | 0.538 |
| ADICP (%DM) | −0.00 | 0.982 | 0.03 | 0.875 | 0.10 | 0.601 | −0.11 | 0.547 | 0.07 | 0.709 | −0.05 | 0.792 | 0.12 | 0.518 | 0.04 | 0.835 | 0.11 | 0.573 | −0.10 | 0.608 |
| ADICP (%CP) | 0.00 | 1.000 | 0.01 | 0.971 | 0.07 | 0.705 | −0.08 | 0.664 | 0.03 | 0.890 | −0.13 | 0.489 | 0.19 | 0.326 | −0.01 | 0.959 | 0.05 | 0.777 | −0.09 | 0.644 |
| CHO profile | ||||||||||||||||||||
| CHO (%DM) | −0.19 | 0.304 | −0.17 | 0.363 | 0.10 | 0.596 | −0.24 | 0.201 | −0.15 | 0.432 | −0.03 | 0.866 | −0.14 | 0.475 | −0.12 | 0.535 | −0.14 | 0.477 | 0.11 | 0.575 |
| Sugar (%DM) | 0.36 | 0.054 | 0.28 | 0.128 | 0.26 | 0.173 | −0.06 | 0.751 | 0.19 | 0.316 | 0.15 | 0.444 | −0.10 | 0.613 | 0.16 | 0.392 | 0.17 | 0.367 | −0.03 | 0.869 |
| Sugar (%NFC) | 0.45 | 0.014 | 0.36 | 0.049 | 0.23 | 0.226 | 0.02 | 0.923 | 0.27 | 0.156 | 0.16 | 0.394 | 0.01 | 0.941 | 0.21 | 0.255 | 0.26 | 0.164 | −0.17 | 0.365 |
| NDF (%DM) | −0.34 | 0.068 | −0.18 | 0.344 | 0.05 | 0.780 | −0.16 | 0.393 | −0.08 | 0.692 | 0.00 | 0.984 | −0.11 | 0.572 | −0.09 | 0.648 | 0.00 | 0.990 | −0.05 | 0.796 |
| ADF (%DM) | −0.30 | 0.113 | −0.10 | 0.600 | 0.02 | 0.900 | −0.08 | 0.683 | 0.05 | 0.814 | 0.08 | 0.672 | −0.13 | 0.486 | −0.01 | 0.977 | 0.16 | 0.408 | −0.14 | 0.477 |
| ADF (%NDF) | 0.08 | 0.678 | 0.11 | 0.561 | 0.06 | 0.738 | 0.02 | 0.906 | 0.15 | 0.443 | 0.17 | 0.367 | −0.12 | 0.525 | 0.12 | 0.538 | 0.21 | 0.276 | −0.06 | 0.771 |
| ADL (%DM) | 0.12 | 0.514 | 0.13 | 0.484 | −0.08 | 0.677 | 0.22 | 0.233 | 0.05 | 0.801 | −0.24 | 0.205 | 0.37 | 0.047 | 0.03 | 0.871 | 0.08 | 0.692 | −0.06 | 0.767 |
| ADL (%NDF) | 0.35 | 0.060 | 0.28 | 0.137 | −0.07 | 0.711 | 0.31 | 0.093 | 0.13 | 0.510 | −0.21 | 0.275 | 0.43 | 0.019 | 0.15 | 0.441 | 0.13 | 0.490 | 0.05 | 0.799 |
| Hemi (%DM) | −0.14 | 0.468 | −0.12 | 0.529 | 0.01 | 0.973 | −0.10 | 0.593 | −0.13 | 0.506 | −0.12 | 0.534 | 0.06 | 0.737 | −0.10 | 0.617 | −0.15 | 0.424 | 0.04 | 0.816 |
| Cell (%DM) | −0.33 | 0.074 | −0.17 | 0.383 | 0.08 | 0.694 | −0.23 | 0.215 | −0.01 | 0.968 | 0.24 | 0.209 | −0.38 | 0.037 | −0.05 | 0.803 | 0.05 | 0.800 | −0.03 | 0.858 |
| NFC (%DM) | −0.18 | 0.347 | −0.21 | 0.273 | −0.01 | 0.952 | −0.12 | 0.517 | −0.20 | 0.301 | −0.07 | 0.725 | −0.13 | 0.492 | −0.15 | 0.445 | −0.22 | 0.233 | 0.26 | 0.162 |
| NFC (%CHO) | −0.10 | 0.606 | −0.15 | 0.426 | −0.08 | 0.667 | 0.00 | 0.994 | −0.17 | 0.372 | −0.07 | 0.713 | −0.12 | 0.533 | −0.10 | 0.608 | −0.22 | 0.239 | 0.33 | 0.078 |
| NSC (%DM) | 0.36 | 0.054 | 0.28 | 0.128 | 0.26 | 0.173 | −0.06 | 0.751 | 0.19 | 0.316 | 0.15 | 0.444 | −0.10 | 0.613 | 0.16 | 0.392 | 0.17 | 0.367 | −0.03 | 0.869 |
Notes: * DM, dry matter; CP, crude protein; SCP, soluble crude protein; NPN, non-protein nitrogen; NDICP, neutral detergent-insoluble crude protein; ADICP, acid detergent-insoluble crude protein; CHO, total carbohydrate; NDF, neutral detergent fiber; ADF, acid detergent fiber; ADL, acid detergent lignin; Hemi, Hemicellulose (Hemicellulose = NDF − ADF); Cell, Cellulose (Cellulose = ADF − ADL); NFC, non-fiber carbohydrate; NSC, non-structural carbohydrate. ** AI_II_T, Amide I and II peak area; AI, Amide I area; AII, Amide II area; R_AAI_II, Area ratios of amide I and II; AIH, Amide I height; AIIH, Amide II height; R_HAI_II, Height ratios of amide I and II; alpha, α-helix height; beta, β-sheet height; R_Ha_b, Ratio α-helix:β-sheet. *** r: correlation coefficient calculated using Spearman method.