Table 1. Larger cargos (3XFLAG) could be inserted with high knock-in frequency at mouse Sox2 locus.
Table 1—source data 1. Raw data for epitope tag knock-in efficiency using 3XFLAG, HA, V5, and Myc single-stranded donor DNA templates.
elife-35069-table1-data1.xlsx (33.1KB, xlsx)
DOI: 10.7554/eLife.35069.008
| Mouse NS cells (ANS4) | |||
|---|---|---|---|
| Tag | Tag size (bp) | Homology arms (bp) | Knock-in efficiency (%) |
| 3XFLAG | 66 | 67 | 21.2 |
| V5 | 42 | 79 | 17.6 |
| Myc | 30 | 85 | 14.9 |
| HA | 27 | 86 | 15.8 |
| Mouse NS cells (BL6) | |||
| Tag | Tag size (bp) | Homology Arms (bp) | Knock-in efficiency (%) |
| 3XFLAG | 66 | 67 | 13.7 |
| V5 | 42 | 79 | 13.9 |
| Myc | 30 | 85 | 9 |
| HA | 27 | 86 | 14.6 |