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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Traffic. 2018 Apr 19;19(6):406–420. doi: 10.1111/tra.12565

Figure 6. Rab11 depletion does not affect TbCatL trafficking.

Figure 6

Transport of lysosomal TbCatL was assayed in control and TbRab11 silenced cells. A. Cells were pulse-chase (10 min/30 min) radiolabeled and at the indicated times TbCatL was immunoprecipitated from cell lysates and analyzed by SDS-PAGE/phosphorimaging. Top. A representative image is presented (107 cell equivalents per lane). Arrival in the lysosome is indicated by loss of precursor (I + X) and appearance of mature (M) forms. White stripes indicate irrelevant portions of the gel that were digitally excised after image processing. Bottom. Results were quantified and expressed as percentage of initial signal (I + X) at T0 (mean ± std. dev., n=3 biological replicates).