FIG 3 .

SAMHD1 is the major downstream effector of diverse IFN types. (A) Representative Western blot analysis of MDM extracts not treated with VLP or treated with Vpx(−)VLP and Vpx(+)VLP and probed for pSAMHD1-T592, total SAMHD1, and β-actin. (B) Representative flow cytometric analysis of viable MDM infected with a replication-incompetent HIV-1ΔEnv-GFP/VSVG in the presence or absence of 50 ng/ml IFN-α, IFN-ε, IFN-γ, or IFN-λ (rows) and treated with the Vpx VLP indicated (columns). Numbers in the graphs indicate the percentage of events falling within the GFP-positive gate as established with mock-infected MDM. (C) Western blot analysis of pSAMHD1-T592, total SAMHD1, and actin for macrophages from donor A051. (D) Bar graphs showing macrophages from three additional, independent donors with each condition conducted in triplicate. (E) Macrophages from donors A006 and A026 were treated with IFN, exposed to 10 mM dN (+), and then infected with HIV-1ΔEnv-GFP/VSVG. Bars indicate the percent GFP-positive cells within the viable population as measured by flow cytometry.