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. 2018 Apr 24;9(31):21641–21654. doi: 10.18632/oncotarget.24956

Figure 3. HPV16-E6 and HPV16-E7 detection limits in defined amounts of cancer cells using RNA extracted from fresh samples.

Figure 3

RNA was extracted from fresh populations of CaSki (HPV16-positive) and HCA2-hTERT (HPV16-negative) cells mixed at the indicated proportions and used for reverse-transcription and TaqMan qPCR. The percentage of CaSki cells in the sample is indicated. (A) Cell populations were analyzed for HPV16-E6 expression. (B) Cell populations were analyzed for HPV16-E7 expression. Note that the internal reference control, 18 s ribosomal RNA, was stably detected in all samples except water (not detected, ND), while HPV16-E6/E7 mRNA levels were gradually reduced according to CaSki cell dilutions. The data is presented as target mRNA expression relative to control (18 s RNA). A representative experiment with mean value ± SD of 3 replicates is presented. *represents the highest dilution factor with statistical significance (P < 0.05, Students T-test) when compared to 0% dilution.