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. 2018 May 1;9(33):23289–23305. doi: 10.18632/oncotarget.25289

Figure 2. STn+ and CD133+ cells have enhanced colony formation capacity and are enriched in spheres.

Figure 2

(A) The indicated sub-populations of OV90, OVCAR3 or OVCAR4 cells were isolated by FACS and seeded in soft agar. Unsorted OV90, OVCAR3 or OVCAR4 cells were seeded in parallel as a control. After 21 days, colonies ≥20 cells were counted and the percent colony formation efficiency of each population was calculated as described in Material and Methods and compared to that of unsorted cells. (B) OV90, OVCAR3 and OVCAR4 cells were cultured under either monolayer or sphere conditions for 10-12 days. At the end of this incubation period, the relative frequency of each sub-population was determined by flow cytometry. Error bars represent the mean ± SEM of a minimum of three independent experiments *p < 0.05.