Table 4.
Oligonucleotides used in this study. Italics and lower case indicate nucleotide sequences added for fusion purposes. Positions are referred to the start codon, (+) downstream or (-) upstream of ATG. Orientation is indicated, (s) sense, (as) antisense.
| Name | Sequence (5′→3′) | Position from ATG | Experimental use |
|---|---|---|---|
| ChsV-1 | CAAGGACGAAAAGAGAGTAAC | + 2769 (s) | RT-PCR |
| ChsV-3 | TGTTGGTAGTTGTGATAATCGT | + 2985 (as) | RT-PCR |
| B2m-F | TTTTCATCTGTCTTCCCCTGT | + 3105 (s) | RT-PCR |
| B2m-R | GTATGTATCAGTCTCAGTGGG | + 3362 (as) | RT-PCR |
| pyrG-F2 | TGCCTCAGCATTGGTACTTG | - 670 (s) | Deletion vector |
| pyrG-R2 | GTACACTGGCCATGCTATCG | + 1335 (as) | Deletion vector |
| 112092-F1 | TGAGTTCTGCTTCTTCGTCTG | - 970 (s) | Deletion vector and probe |
| 112092-R1 | caagtaccaatgctgaggcaGGGGCTTTCTGGATGATGAGT | - 19 (as) | Deletion vector and probe |
| 112092-F2 | cgatagcatggccagtgtacGTAGTAGCGTTGAATAAAGAGT | + 1327 (s) | Deletion vector |
| 112092-R2 | GTGAGTTTCTGCTTGAATTTGT | + 2456 (as) | Deletion vector |
| 112092-F3n | ACGAAGAATCAGCAATTACACA | - 917 (s) | Deletion vector |
| 112092-R3n | ACTCCATCAACTTTTATAAGCC | + 2398 (as) | Deletion vector |
| 108920-F1 | TTTTGCAGGCTTTTATTACATTT | - 1063 (s) | Deletion vector |
| 108920-R2 | ACCTATGCAATGCCTCCGATGCGA | - 791 (s) | Deletion vector |
| 108920-F2 | cgatagcatggccagtgtacCCATTGGCATCACGGATCAGCA | + 563 (as) | Deletion vector |
| 108920-R1 | caagtaccaatgctgaggcaTGCTGCTGCGTACTGACTGATGT | + 2312 (s) | Deletion vector and probe |
| 108920-F1 | GATGCAGGTTGTGATGGAAC | + 3123 (as) | Deletion vector and probe |