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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Hypertension. 2018 Jan 22;71(3):518–529. doi: 10.1161/HYPERTENSIONAHA.117.10503

Figure 1.

Figure 1

STIM2 protein expression is upregulated in PASMC from patients with PAH compared with PASMC from normal subjects and is sufficient and necessary for increasing the resting [Ca2+]cyt in PASMCs. A Representative fluorescent images showing the resting [Ca2+]cyt in a normal PASMC and a PAH PASMC. B, Histogram (left) showing the distribution of the resting [Ca2+]cyt in normal (n=3 normal subjects) and PAH (n=5 patients) PASMCs. Summarized data (right, means±SE) showing the averaged resting [Ca2+]cyt in normal and PAH PASMCs. ***P<0.001 vs. Normal. C, Western blot analyses on STIM2 (left) in normal and PAH PASMCs. Summarized data (right, means±SE, n=3 normal subjects, and 4 PAH patients) showing the protein level of STIM2 in normal and PAH PASMCs. *P<0.05 vs. Normal. D, Representative fluorescent images (a) showing the resting [Ca2+]cyt in normal PASMCs and normal PASMCs transfected with GFP-vector and STIM2 gene (STIM2-YFP). Arrows indicate the normal PASMCs that are positively transfected with STIM2-YFP. Histogram (b) showing the distribution of the resting [Ca2+]cyt in Control, GFP-vector-transfected and STIM2-YFP-transfected normal PASMCs. Vertical arrows indicate the peak values of the resting [Ca2+]cyt. Summarized data (c, means±SE, n=3 normal subjects) showing the averaged resting [Ca2+]cyt in Control, Vector-transfected and STIM2-transfected normal PASMCs. ***P<0.001 vs. Control and GFP-vector. E, Representative fluorescent images (a) showing the resting [Ca2+]cyt in PAH-PASMCs (Control) and PAH-PASMCs transfected with a Control-CRISPR/Cas9 plasmid (Control-CRISPR/Cas9) and the STIM2-CRISPR/Cas9 plasmid (STIM2-CRISPR/Cas9). Arrows indicate the PAH-PASMCs that are positively transfected with STIM2-CRISPR/Cas9 plasmid. Histogram (b) showing the distribution of the resting [Ca2+]cyt in Control, Control-CRISPR/Cas9-transfected and STIM2-CRISPR/Cas9-transfected PAH-PASMCs, with the averaged values of the resting [Ca2+]cyt at 138.2, 132.5 and 91.8 nM, respectively. Summarized data (c, means±SE, n=5 PAH patients) showing the level of the resting [Ca2+]cyt in Control, Control-CRISPR/Cas9-transfected and STIM2-CRISPR/Cas9-transfected PAH-PASMC. ***P<0.001 vs. Control and Control-CRISPR/Cas9.