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. 2017 Dec 25;67(2):201–218. doi: 10.1538/expanim.17-0112

Deletion of both p62 and Nrf2 spontaneously results in the development of nonalcoholic steatohepatitis

Kentaro Akiyama 1,2,*, Eiji Warabi 3,*, Kosuke Okada 4, Toru Yanagawa 5, Tetsuro Ishii 3, Katsumi Kose 6, Katsutoshi Tokushige 7, Kazunori Ishige 4, Yuji Mizokami 4, Kenji Yamagata 5, Kojiro Onizawa 5, Shun-ichi Ariizumi 8, Masakazu Yamamoto 8, Junichi Shoda 9
PMCID: PMC5955752  PMID: 29276215

Abstract

Nonalcoholic steatohepatitis (NASH) is one of the leading causes of chronic liver disease worldwide. However, details of pathogenetic mechanisms remain unknown. Deletion of both p62/Sqstm1 and Nrf2 genes spontaneously led to the development of NASH in mice fed a normal chow and was associated with liver tumorigenesis. The pathogenetic mechanism (s) underlying the NASH development was investigated in p62:Nrf2 double-knockout (DKO) mice. DKO mice showed massive hepatomegaly and steatohepatitis with fat accumulation and had hyperphagia-induced obesity coupled with insulin resistance and adipokine imbalance. They also showed dysbiosis associated with an increased proportion of gram-negative bacteria species and an increased lipopolysaccharide (LPS) level in feces. Intestinal permeability was elevated in association with both epithelial damage and decreased expression levels of tight junction protein zona occludens-1, and thereby LPS levels were increased in serum. For Kupffer cells, the foreign body phagocytic capacity was decreased in magnetic resonance imaging, and the proportion of M1 cells was increased in DKO mice. In vitro experiments showed that the inflammatory response was accelerated in the p62:Nrf2 double-deficient Kupffer cells when challenged with a low dose of LPS. Diet restriction improved the hepatic conditions of NASH in association with improved dysbiosis and decreased LPS levels. The results suggest that in DKO mice, activation of innate immunity by excessive LPS flux from the intestines, occurring both within and outside the liver, is central to the development of hepatic damage in the form of NASH.

Keywords: dysbiosis, hyperphagia, intestinal permeability, lipopolysaccharide, multiple parallel hits hypothesis

Introduction

Nonalcoholic steatohepatitis (NASH) is a progressive liver disease characterized by steatosis, inflammation, and fibrosis leading to liver cirrhosis and cancer [3]. NASH, a hepatic representation of metabolic syndrome, is an increasingly common health problem globally [40].

It is unlikely that the pathogenesis of NASH is dependent upon any single factor, and it is likely that NASH is the result of multiple factors, including obesity/insulin resistance-mediated steatosis, intestinal-derived lipopolysaccharides (LPS), oxidative stress, lipotoxicity, and other conditions that promote inflammation, fibrosis, and hepatocyte death [7, 38]. The molecular mechanisms corresponding to these pathogenic processes are poorly understood.

p62/A170/Sqstm1 is a cytoplasmic endosome-associated protein that acts as a scaffold for atypical protein kinase C [17]. Recently, p62 was reported to play an important role in selective autophagy of ubiquitin-binding protein [24, 35]. p62-knockout (p62-KO) mice develop mature-onset obesity after progressing to insulin and leptin resistance when fed a standard diet [33]. In a previous study, hyperphagia (overeating) was reported to be the primary cause of obesity in p62-KO mice and was found to be associated with a disruption in leptin signaling [12].

Nuclear factor E2-related factor-2 (Nrf2), a transcription factor, is a master regulator of the cellular adaptive response to oxidative stress [19, 23, 37]. Nrf2-knockout (Nrf2-KO) mice exhibit a severe deficiency in the gene regulatory program for the antioxidant response, resulting in high susceptibility to oxidative stress-related disorders and chemical carcinogenesis [20]. In a previous study, Nrf2-KO mice were found to be prone to developing NASH/fatty liver disease when fed a diet deficient in methionine and choline [32]. It is reported that activation of Nrf2 prevents LPS-induced transcriptional upregulation of pro-inflammatory cytokines, including interleukin (Il)-6 and IL-1β [22].

Recent animal models of NASH include a diet-induced rat model developed by administration of a methionine- and choline-deficient diet and hepatocyte-specific Pten-deficient mice [15, 45]. However, these animal models show an unnatural decrease in body weight and rapid progression of symptoms, and there is the possibility that they deviate from human NASH. The lack of comparative animal models is a major reason for why the pathogenic mechanisms of NASH have not been clarified, and the development of a novel model is required.

In the current study, we developed a p62:Nrf2 gene double-knockout (DKO) mouse by crossing a p62-KO mouse and an Nrf2-KO mouse and investigated possible new mechanisms associated with the deletion of both p62 and Nrf2 genes in the pathogenesis of NASH. It is likely that p62 and Nrf2 deficiency plays a variety of pathogenic roles in a number of organs and/or tissues that form a basis for NASH onset and progression through a linkage between organs and tissues. We found that the DKO mouse is a novel animal model of NASH.

Materials and Methods

Animal studies

All experiments were performed under protocols approved by the Institutional Animal Care and Use Committees of the University of Tsukuba. Except where indicated in this paper or cited in the respective references, animals were bred and maintained under standard housing conditions with ad libitum access to food (MF: 5.1% fat, 23.1% protein, 360 kcal/100 g; Oriental Yeast, Tokyo, Japan) and water. They were housed at an ambient temperature of 20–23°C on a daily 12-h light/dark cycle with 30–70% relative humidity and were weaned from their mothers between 3–4 weeks of age. All mice were kept under specific pathogen-free conditions in an environmentally controlled clean room at the Laboratory Animal Resource Center, University of Tsukuba.

The mice used were 8-, 30-, and 50-week-old male wild-type (WT) C57BL/6J mice obtained from Charles Laboratories Japan (Kanagawa, Japan) and bred in our own colony. p62-KO and Nrf2-KO mice were generated and genotyped as previously described [18, 24]. DKO mice were produced by crossing these mutant mice and regenotyped. Before analysis, mice were crossed with C57BL/6J mice for>10 generations to produce fertile offspring that grew normally. Male mice were used in all experiments. In pair-feeding experiments, one 3-week-old DKO pup was weaned in a separate cage and given a portion of standard diet (3.0 ± 0.1 g) per day until 25 weeks of age [12]. In probiotics experiments, DKO mice were administered probiotics (VSL#3, VSL Pharmaceuticals, Gaithersburg, MD, USA) from 10 weeks of age and observed at 25 weeks of age. VSL#3 was dissolved in drinking water (4.5 billion colonies/ml).

Glucose tolerance test

Mice were fasted for 13 h before intraperitoneal injection of glucose (1 g/kg body weight). A drop of blood drop was taken from the tail after 15, 30, 60, and 120 min. The tail tip was cut 2 mm from the end, and the tail was pressed to collect a drop of blood at the indicated time. Glucose levels were measured using a portable glucose meter (Ascensia, Bayer HealthCare, Berlin, Germany).

Biochemical analyses

Serum glucose was detected using a glucose CII test kit (Wako, Tokyo, Japan). Serum insulin was measured using a mouse insulin ELISA kit (Morinaga, Kanagawa, Japan). Serum leptin was measured using a mouse/rat leptin ELISA kit (Morinaga, Kanagawa, Japan). Adiponectin was measured in serum using a Mouse Adiponectin/Acrp30 Quantikine ELISA Kit (R&D Systems, Minneapolis, MN, USA). The homeostatic model assessment for insulin resistance (HOMA-IR) was determined by performing the following calculation: HOMA-IR=fasting plasma insulin (mU/l) × fasting blood glucose (mg/dl)/405.

Histological analysis

Liver, intestine, and adipose tissues were fixed in 4% paraformaldehyde and embedded in paraffin, and 2 µm-thick tissue sections were stained with hematoxylin-eosin (H&E) and sirius red solution. For immunostaining of 4-hydroxy-2-nonenal (4-HNE), sections were stained using the indirect immunoperoxidase method with anti-4HNE monoclonal antibodies (Ab) (JaiCA, Shizuoka, Japan). For immunostaining of glutathione S-transferase P1, tissue sections were immunostained with mAb (BML, Tokyo, Japan). To determine the histopathological lesions in nonalcoholic fatty liver disease, the steatosis, activity, and fibrosis (SAF) score were assessed separately for the grade of steatosis (from S0 to S3), the activity (from A0 to A4 by adding grades of ballooning and lobular inflammation, both from 0 to 2), and the stage of fibrosis (from F0 to F4) [2]. Histological changes in the intestines were evaluated and graded by a blinded evaluator as follows: 0 (normal), no damage; 1 (mild), slight submucosal and/or lamina propria separation; 2 (moderate), moderate separation of the submucosa and/or lamina propria and/or edema in the submucosa and muscular layers; 3 (severe), severe separation of the submucosa and/or lamina propria and/or severe edema in the submucosa and muscular layers with regional villous sloughing; and 4 (necrosis), loss of villi and necrosis [28].

LPS levels

Concentrations of LPS in plasma and feces were evaluated using a Pyrochrome limulus amebocyte lysate assay (Associates of Cape Cod, East Falmouth, MA, USA). LPS levels are expressed as Endotoxin Units (EU).

Fecal bacterial analysis

Stool samples were collected from mice, snap-frozen and stored at −80°C until use. DNA extraction from fecal samples and analysis of the fecal microbiota using a 16S rDNA library and terminal restriction fragment length polymorphisms were outsourced to TechnoSuruga Laboratory Co., Ltd. (Shizuoka, Japan). Preparation of the fecal microbiota using a 16S rRNA gene amplicon library and analysis of 16S rRNA gene amplicon sequencing were outsourced to FASMAC Co., Ltd. (Kanagawa, Japan).

Magnetic resonance imaging (MRI) analyses

For analysis of Kupffer cells phagocytosis, MRI was performed using a 1T animal scanner (MRTechnology, Ibaraki, Japan). Region-of-interest measurements of T2 values were performed on consecutive pre- and superparamagnetic iron oxide (SPIO) administration.

Evaluation of microsphere phagocytosis

Prior to 5 min of liver perfusion, latex beads (1.0 µm diameter, carboxylate-modified; FluoSpheres®, Thermo Fisher Scientific, Waltham, MA, USA) at 0.57 µl/g body weight were injected into the tail vein. Following liver perfusion, cells were isolated. The Kupffer cell surface marker F4/80 was stained. The percentage of phagocytic cells and the fluorescence intensity of latex beads in F4/80-positive cells were evaluated by flow cytometry.

Flow cytometric analysis

Kupffer cells were stained with APC-conjugated anti-F4/80 Ab (Thermo Fisher Scientific, Waltham, MA, USA), PerCP/Cy5.5 anti-mouse CD206 Ab (BioLegend, San Diego, CA, USA), PE anti-mouse CD11c Ab (Biolegend, CA, USA), anti-macrophage receptor with collagenous structure (MARCO) Ab (Bio-Rad, Hercules, CA, USA), and anti-class A macrophage scavenger receptor (SR-A) Ab (R&D Systems, Minneapolis, MN, USA) with Alexa Fluor 488 (Thermo Fisher Scientific, Waltham, MA, USA). Flow cytometry was performed using a Gallios flow cytometer (Beckman Coulter, Brea, CA, USA).

Intestinal permeability assay

To evaluate epithelial permeability in the intestines, mice underwent gavage with nondigestible 4 kDa fluorescein isothiocyanate (FITC)-dextran (Sigma-Aldrich, St. Louis, MO, USA) at a dose of 0.5 mg/g body weight after fasting for 13 h. Serum (50 µl) was collected from the fundus at various time points (0, 1, 2, 3, and 6 h), and the intensity of FITC in serum was measured using an iMark plate reader (Bio-Rad, Hercules, CA, USA) with excitation at 488 nm. Data were plotted against the standard curve of serial dilutions of FITC-dextran.

Immunoblot analysis

After extraction from mouse samples or cultured cells, each protein sample was subjected to SDS/PAGE and transferred to a PVDF membrane (Bio-Rad, Hercules, CA, USA). Then the corresponding primary and secondary antibodies were incubated to visualize the protein. All antibodies used in this study are listed in Supplementary Table 1.

Quantitative real-time polymerase chain reaction (qRT-PCR)

Total RNA was extracted from specimens or cultured cells, followed by cDNA synthesis. qRT-PCR was performed with Fast SYBR Green Master Mix (Thermo Fisher Scientific, Waltham, MA, USA). Data were normalized to the amount of Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) present in each sample and then averaged. Supplementary Table 2 provides a list of all the primers used in this study.

In vitro tissue culture studies and cell lines

Mouse primary Kupffer cells from 8- to 10-week-old male WT, Nrf2-KO, p62-KO, and DKO mice were isolated via portal vein collagenase treatment (Worthington Biochemical, Lakewood, NJ, USA) followed by centrifugation and cell sorting. For Kupffer cell sorting, cells were incubated with APC-conjugated anti-F4/80 Abs (Thermo Fisher Scientific, Waltham, MA, USA) for 30 min. Cells were sorted into F4/80+ subpopulations using a Beckman Coulter MoFlo XDP cell sorter and immediately processed for RNA isolation.

The murine monocyte/macrophage cell line RAW264.7 was obtained from ATCC. The human colon cancer cell line Caco-2 was purchased from RIKEN BRC (Ibaraki, Japan). Cells were cultured at 37°C under 5% CO2 in high glucose Dulbecco’s modified Eagle’s medium containing 4.5 g/l glucose, 1% penicillin-streptomycin, 10% fetal bovine serum, and 1% nonessential amino acids (only in Caco-2).

Knockout of p62 and Nrf2 genes using the CRISPR-Cas9 system

The detailed instructions of a published protocol were followed to knock out p62 and Nrf2 genes using the CRISPR-Cas9 system [5]. Briefly, 20 bp target single-guide RNA sequences were obtained by screening p62 and Nrf2 mRNA sequences using the CRISPR Design online software (http://crispr.mit.edu/). After annealing, short double-strand DNA was ligated using the BbsI digested pX330 plasmid. RAW264.7 and Caco-2 cells were transfected with individual pX330 gDNA using the Neon Transfection System.

Measurement of transepithelial electrical resistance (TER)

Caco-2 cells were seeded into a polyethylene terephthalate membrane with 0.4 µm pores. The medium was changed every other day until complete differentiation. Electrical resistance was measured using a Millicell-ERS electrical resistance system (EMD Millipore, Billerica, MA, USA). TER values are expressed as Ω·cm2. Measurements were performed on three replicates in three independent experiments. In the LPS experiments, cell monolayers were treated with 100 EU /ml LPS for 3 or 6 h, and TER was measured.

Statistical analysis

Statistical analysis was conducted using IBM SPSS Statistics 22.0 (IBM, Armonk,, NY, USA). Values are expressed as the mean ± SE. When two groups were compared, the unpaired t-test was used for data analysis. Multiple-group comparisons were performed using one-way analysis of variance (ANOVA). A P-value<0.05 was considered statistically significant.

Results

DKO mice fed a normal chow diet exhibited hyperphagia, obesity, insulin resistance, and adipokine imbalance

DKO mice were negative for p62 and Nrf2 protein expression in the liver (Fig. 1A). The body weights of WT, Nrf2-KO, p62-KO, and DKO mice fed a normal chow diet were monitored over a 42-week period in mice 8–50 weeks of age. Mice lacking p62 and/or Nrf2 genes were viable and had similar body weights at birth. Thereafter, p62-KO and DKO mice gained weight much faster than WT and Nrf2-KO mice (Fig. 1B). At 30 weeks of age, the body weights for each genotype were 37.8 ± 1.3 g for WT, 37.6 ± 1.0 g for Nrf2-KO, 49.7 ± 1.3 g for p62-KO, and 46.5 ± 0.6 g for DKO. Liver mass was significantly increased in DKO mice compared with in WT mice (Supplementary Fig. 1A) but was less than that in p62-KO mice. Using computed tomography, percentages of body fat and visceral fat weight were observed to increase in p62-KO and DKO mice (Supplementary Fig. 1B). WT and Nrf2-KO mice showed only a slight increase in food intake with age, with the average intake at 8 weeks being 3.0 g/day/mouse. In contrast, food intake of p62-KO and DKO mice increased from 3.3 g/day/mouse at 8 weeks of age to 4.0 g/day/mouse at 30 weeks of age (Fig. 1C).

Fig. 1.

Fig. 1.

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p62:Nrf2 gene double-knockout (DKO) mice fed a normal chow diet exhibited hyperphagia, obesity, insulin resistance, and adipokine imbalance. (A) Immunoblot analysis of Nrf2 and p62 in livers of wild-type (WT), Nrf2-knockout (KO), p62-KO, and DKO mice at 8 weeks of age. (B) Body weight changes in WT, Nrf2-KO, p62-KO, and DKO mice (n=10–15 per group). (C) Food intake, (D) fasting blood glucose concentration, fasting serum insulin concentration, and homeostatic model assessment for insulin resistance (HOMA-IR) (n=8 per group) at 8 and 30 weeks of age. (E) Glucose tolerance tests were performed after mice were fasted for 13 h at 8 and 30 weeks of age (n=7–9 per group). (F) Serum leptin and adiponectin concentrations at 8 and 30 weeks of age (n=8 per group). Results are presented as the mean ± SE. *P<0.05, significantly different from the WT group; †P<0.05, significantly different from the Nrf2-KO group; §P<0.05, significantly different from the p62-KO group.

In comparisons of blood biochemistry versus the WT mice, DKO mice showed the development of hyperglycemia and a tendency towards increased fasting insulin levels and homeostatic model assessment for insulin resistance (HOMA-IR). Of note, DKO mice exhibited insulin resistance at 8 weeks of age (Fig. 1D). In addition, DKO mice exhibited glucose intolerance in an intraperitoneal glucose tolerance test (Fig. 1E). Serum leptin levels in p62-KO and DKO mice increased significantly from 8 weeks to 30 weeks of age compared with WT and Nrf2-KO mice. Serum adiponectin levels in p62-KO and DKO mice decreased significantly (Fig. 1F).

Collectively, the results indicated that DKO mice developed hyperphagia and obesity (visceral fat accumulation) and exhibited insulin resistance and adipokine imbalance.

DKO mice fed a normal chow diet developed NASH and liver tumors

The time course of changes in liver histology for WT, Nrf2-KO, p62-KO, and DKO mice are shown in Figs. 2A, 2B, and Supplementary Fig. 2A p62-KO and DKO mice exhibited steatosis at 30 weeks of age (Fig. 2C). DKO mice showed a marked increase in infiltration of inflammatory cells in the liver compared with WT mice (Fig. 2D). Hepatic fibrosis significantly increased in DKO mice (Fig. 2E). In contrast, no marked increase in hepatic fibrosis was observed in Nrf2-KO and p62-KO mice (Fig. 2E). The extent of fibrosis was confirmed by Masson’s trichrome staining and was closely associated with hepatic fibrosis (Fig. 2F). While steatosis, activity, and fibrosis (SAF) scores increased in Nrf2-KO and p62-KO mice, scores showed greater increases in DKO mice (Fig. 2C-E). Immunostaining with 4-HNE was performed to determine the presence of lipid peroxides. Increased staining was observed in the livers of DKO mice at 30 weeks of age but not in Nrf2-KO and p62-KO mice (Supplementary Fig. 2B).

Fig. 2.

Fig. 2.

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p62:Nrf2 gene double-knockout (DKO) mice developed nonalcoholic steatohepatitis and liver tumors. (A) Hematoxylin and eosin (H&E)-stained sections of representative liver specimens from wild-type (WT), Nrf2-knockout (KO), p62-KO, and DKO mice at 8 and 30 weeks of age. Arrowheads indicate infiltration of inflammatory cells (scale bar, 100 µm). (B) Sirius red-stained sections. (C–E) The steatosis, activity, and fibrosis (SAF) scores for each group are shown (n=8 per group). (F) Fibrotic areas in livers of WT, Nrf2-KO, p62-KO, and DKO mice (n=8 per group). (G) Representative liver tumors in DKO mice. Macroscopic view of tumors (upper panel), an H&E-stained section (middle panel; T, tumor part; NT, non-tumor part), and a glutathione S-transferase P1-stained section of the tumor (lower panel) (scale bar, 100 µm). The inset areas show magnified views of part of the tumor in the middle panels (scale bar, 10 µm). Results are presented as the mean ± SE. *P<0.05, significantly different from the WT group; †P<0.05, significantly different from the Nrf2-KO group; §P<0.05, significantly different from the p62-KO group.

Tumor formation was observed in the livers of DKO mice. Macroscopic liver tumors were observed in 12% (8/66) of DKO mice by 50 weeks of age (Fig. 2G). No tumors were observed in Nrf2-KO and p62-KO mice. Microscopic findings revealed that tumors were comprised of cellular masses with nuclear atypia, included lipid droplets in some cells, and were partially surrounded by a fibrous cap. Intense glutathione S-transferase P1 staining was observed in whole sections of tumors.

With regard to visceral fat tissue, crown-like structures of inflammatory cells, comprising macrophages surrounding dead or dying adipocytes (a histologic hallmark of the pro-inflammatory process), were observed in p62-KO and DKO mice, with greater inflammatory cell infiltration being observed in DKO mice (Supplementary Fig. 2C).

Hepatic expression levels of tumor necrosis factor-α (Tnf-α), IL-1β, IL-6, and toll-like receptor 4 (Tlr4) mRNA increased in DKO mice at 8 and 30 weeks of age compared with WT mice. mRNA levels of transforming growth factor-β1 (Tgf-β1) and procollagen-α1, both of which are closely associated with hepatic fibrosis, increased in DKO mice at 30 weeks of age (Table 1). Visceral fat expression levels of IL-1β mRNA significantly increased in DKO mice at 8 weeks of age. mRNA levels of Tnf-α, IL-1β, Tlr4, monocyte chemoattractant protein 1 (Mcp-1), CD14, and Tgf-β1 increased in DKO mice at 30 weeks of age compared with WT mice (Table 1).

Table 1. Relative mRNA expression levels determined by qPCR for pathophysiological factors in the liver and visceral fat tissue of mice at 8 and 30 weeks of age.

Age 8 weeks 30 weeks
Genes WT Nrf2-KO p62-KO DKO WT Nrf2-KO p62-KO DKO
Liver
Tnf-α 1.00 ± 0.10 1.11 ± 0.17 1.60 ± 0.07 a 3.09 ± 0.84 ab 2.47 ± 0.79 3.02 ± 0.70 4.25 ± 0.37 ab 5.43 ± 1.01 ab
IL-1β 1.00 ± 0.17 0.85 ± 0.08 2.69 ± 0.31 ab 3.52 ± 0.41 ab 1.68 ± 0.26 2.02 ± 0.15 3.19 ± 0.36 ab 4.18 ± 0.32 ab
IL-6 1.00 ± 0.25 1.27 ± 0.07 2.99 ± 0.32 ab 2.49 ± 0.28 ab 1.39 ± 0.57 0.97 ± 0.32 2.66 ± 0.77 ab 4.14 ± 0.96 ab
Tlr-4 1.00 ± 0.17 1.16 ± 0.06 1.09 ± 0.09 1.43 ± 0.12 abc 1.69 ± 0.23 1.82 ± 0.29 4.02 ± 0.35 ab 3.84 ± 0.43 ab
Tgf-β1 1.00 ± 0.15 1.07 ± 0.08 0.86 ± 0.05 1.35 ± 0.15 0.97 ± 0.71 0.98 ± 0.36 1.49 ± 0.61 1.82 ± 0.72 ab
Procollagen-α1 1.00 ± 0.16 1.26 ± 0.23 0.83 ± 0.13 1.24 ± 0.19 1.14 ± 0.73 1.30 ± 0.65 1.88 ± 0.39 2.27 ± 0.79 a
Visceral fat
Tnf-α 1.00 ± 0.10 1.17 ± 0.27 0.80 ± 0.10 1.30 ± 0.30 1.15 ± 0.26 1.23 ± 0.45 2.48 ± 0.22 5.72 ± 0.78 abc
IL-1β 1.00 ± 0.16 1.47 ± 0.61 1.60 ± 0.34 4.10 ± 0.86 abc 1.92 ± 0.52 6.92 ± 2.14 4.20 ± 1.22 32.26 ± 12.83 abc
Tlr-4 1.00 ± 0.06 0.93 ± 0.16 0.88 ± 0.11 0.81 ± 0.02 0.90 ± 0.08 0.98 ± 0.15 0.89 ± 0.08 1.66 ± 0.18 abc
Mcp-1 1.00 ± 0.11 2.26 ± 0.54 1.40 ± 0.20 1.75 ± 0.46 1.09 ± 0.12 1.31 ± 0.38 3.70 ± 1.11 15.55 ± 4.84 abc
Cd14 1.00 ± 0.18 0.64 ± 0.17 0.65 ± 0.13 0.48 ± 0.06 0.63 ± 0.04 0.70 ± 0.10 0.99 ± 0.27 3.29 ± 0.99 abc
Tgf-β1 1.00 ± 0.12 0.91 ± 0.13 0.85 ± 0.06 0.79 ± 0.07 0.65 ± 0.04 0.81 ± 0.14 0.82 ± 0.12 1.68 ± 0.15 abc
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mRNA expression levels were normalized using the values of the WT group at 8 weeks of age. Values are presented as the mean ± SE (n=8 per group). aP<0.05, significantly different from the WT group; bP<0.05, significantly different from the Nrf2-KO group; cP<0.05, significantly different from the p62-KO group. DKO, p62:Nrf2 gene double-deficient mice; Tnf, tumor necrosis factor; Tlr, toll-like receptor; Mcp, monocyte chemotactic protein; Cd, cluster of differentiation; Tgf, transforming growth factor.

These results suggested that DKO mice first developed liver inflammation at an early age prior to obesity and then developed NASH and liver tumors with aging.

Modified microbiota composition and fecal LPS content in DKO mice

Fecal microbiota composition was determined in DKO mice at 30 weeks of age. Analysis was conducted using culture-independent PCR amplification of the variable 4 region of bacterial 16S rRNA genes followed by Illumina sequencing. Principal coordinates analysis of the weighted UniFrac distance between samples revealed that the microbiota composition was profoundly modified in DKO mice (Fig. 3A). Whereas gram-positive bacteria were the major representative signature operational taxonomic unit accounting for the differences in intestinal microbiota composition in WT mice, gram-negative bacteria were the predominant signature operational taxonomic unit in p62-KO and DKO mice (Fig. 3B). The Porphyromonadaceae and Paraprevotellaceae families were more abundant in DKO than in WT mice, and the Lachnospiraceae and Ruminococcaceae families were less abundant in DKO mice (Fig. 3C).

Fig. 3.

Fig. 3.

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p62:Nrf2 gene double-knockout (DKO) mice showed a modified microbiota composition and increased fecal lipopolysaccharide (LPS) levels. (A) Principal coordinates analysis of the fecal microbiota from wild-type (WT), Nrf2-knockout (KO), p62-KO, and DKO mice at 30 weeks of age. (B) The relative abundance of gram-negative bacteria was calculated as the percentage of bacteria relative to the total bacteria for fecal bacteria (n=8 per group). (C) The relative abundance of families of bacteria in feces was examined. The relative abundance was calculated as the percentage of the bacterial subgroup relative to the total bacteria. (D) Fecal LPS levels in mice (n=8 per group). (E) Serum LPS levels in mice (n=8 per group). Results are presented as the mean ± SE. *P<0.05, significantly different from the WT group; †P<0.05, significantly different from the Nrf2-KO group; §P<0.05, significantly different from the p62-KO group.

LPS is the predominant cause of liver inflammation in NASH patients [4, 46]. Therefore, fecal and serum levels of LPS were determined in mice. Correlating with the microbiota composition, fecal LPS concentrations were higher in p62-KO and DKO mice than in WT mice (Fig. 3D), but serum LPS concentrations were higher only in DKO mice (Fig. 3E).

Intestinal permeability increased in DKO mice

Previous studies have reported that increased intestinal permeability is observed more frequently in NASH patients [30, 34]. In the current study, intestinal tissue sections from WT, Nrf2-KO, p62-KO, and DKO mice were stained with hematoxylin and eosin, and epithelial damage was scored according to the histology. There was marked damage to intestinal epithelial morphology in DKO mice compared with WT mice. At 30 weeks, damage scores increased in Nrf2-KO and p62-KO mice, but they increased to a greater extent in DKO mice (Fig. 4A). Measurement of intestinal permeability by absorption of FITC-dextran showed prominent enhancement in Nrf2-KO and DKO mice compared with WT at 8 weeks of age. Permeability in DKO mice was further increased at 30 weeks of age (Fig. 4B).

Fig. 4.

Fig. 4.

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Intestinal permeability increased in p62:Nrf2 gene double-knockout (DKO) mice. (A) Hematoxylin and eosin (H&E)-stained sections of representative intestine specimens from wild-type (WT), Nrf2-knockout (KO), p62-KO, and DKO mice at 8 and 30 weeks of age (left panel) (scale bar, 100 µm). Histological scores for intestinal epithelia (right panel) (n=8 per group). (B) Area under the plasma concentration curve of fluorescein isothiocyanate (FITC)-dextran after administration of the marker by gavage (n=8 per group). (C) Immunoblot analysis of zona occludens-1 (Zo-1) in the intestines of WT, Nrf2-KO, p62-KO, and DKO mice at 8 weeks of age and quantification of immunoblotting of Zo-1 normalized to actin in mice (n=5 per group). (D) Immunoblot analysis of Nrf2 and p62 in Caco-2 cells of WT, Nrf2-KO, and p62-KO. (E) Barrier function was analyzed by transepithelial electrical resistance (TER). Caco-2 cells were stimulated with 100 EU/ml lipopolysaccharide (LPS) (EU, endotoxin units). TER was tested at different time points (n=5 per group). (F) Quantification of immunoblotting of Zo-1, claudin 1, and claudin 2 normalized to actin in Caco-2 cells (n=5 per group). Results are presented as the mean ± SE. *P<0.05, significantly different from the WT group; †P<0.05, significantly different from the Nrf2-KO group; §P<0.05, significantly different from the p62-KO group.

Intestinal permeability is regulated by tight junctions formed between intestinal epithelial cells at most apical areas of the epithelium. Expression levels of the tight junction protein zona occludens-1 (Zo-1) were evaluated by immunoblotting of intestinal tissue lysates from DKO mice. Expression levels of Zo-1 decreased in the intestines of Nrf2-KO and DKO mice compared with WT mice (Fig. 4C). Expression levels of Claudin 1 and Claudin 2 were not significantly different among the groups of mice (data not shown). mRNA expression levels of intestinal Zo-1, Claudin 1, and Claudin 2 were also not significantly different (Supplementary Fig. 3A).

To investigate the effects of p62 or Nrf2 deficiency on intestinal barrier function, an in vitro model in which Caco-2 epithelial cells were grown in monolayers was constructed. Using the CRISPR-Cas9 system, Caco-2 cells lacking the p62 or Nrf2 gene were also constructed. Expression of p62 and Nrf2 protein in each gene-deficient cell was confirmed by immunoblotting (Fig. 4D). TER is an indicator of epithelial paracellular permeability to ionic solutes and was used to assess intestinal barrier function. Compared with WT cells, TER was consistently decreased in Nrf2-deficient cells (Fig. 4E). The results of immunoblotting showed that the expression of Zo-1 and Claudin 1 protein decreased in Nrf2-deficient cells compared with WT cells (Fig. 4F). Zo-1 mRNA expression levels decreased in Nrf2-deficient cells. Claudin 1 mRNA expression levels were not significantly different (Supplementary Fig. 3B).

Because LPS causes intestinal barrier dysfunction, an in vitro model in which Caco-2 epithelial cells were grown in monolayers was treated with LPS. Compared with vehicle-treated cells, TER was consistently decreased in LPS-treated WT, Nrf2-deficient, and p62-deficient cells at 3 h and 6 h, indicating disruption of the barrier function in the monolayers (Fig. 4E). Of note, Nrf2-deficient cells potently inhibited the reduction in TER caused by treatment with LPS at all time points (Fig. 4E).

Changes in Kupffer cell phenotype in DKO mice

Macrophage polarization is a process by which macrophages express different functional programs in response to microenvironmental signals [29]. In the livers of DKO mice at 8 weeks of age, M1 phenotype Kupffer cells but not M2 phenotype Kupffer cells increased, as determined using flow cytometry for CD11c as a marker for M1 macrophages and CD206 as a marker for M2 macrophages (Fig. 5A).

Fig. 5.

Fig. 5.

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Change in Kupffer cell phenotype in p62:Nrf2 gene double-knockout (DKO) mice. (A) CD11c and CD206 expression in F4/80+ cells (Kupffer cells) in the liver. Numbers in panels and quadrants are percentages of the indicated populations. (B) Kupffer cell phagocytic function was determined by superparamagnetic iron oxide (SPIO) magnetic resonance imaging (MRI). The SPIO signal was calculated by MRI (reflecting the T2 value), and then the phagocytic function was evaluated. (C) Phagocytic activity of F4/80+ cells in the liver. Isolated liver F4/80+ cells were incubated with fluorescein isothiocyanate (FITC) microspheres, and then FITC microsphere-positive cells were analyzed. MARCO and SR-A expression in F4/80+ cells in the liver. (D) Immunoblot analysis of Nrf2 and p62 in RAW264.7 cells of wild-type (WT), Nrf2-knockout (KO), p62-KO, and DKO. (E) Immunoblot analysis of phosphorylated NF-kB p65 (p-NF-kB p65) and total NF-kB p65 in RAW264.7 cells of WT, Nrf2-KO, p62-KO, and DKO and quantification of immunoblotting of p-NF-kB p65 normalized to total NF-kB p65. Cells were stimulated with 10 EU/ml lipopolysaccharide (LPS) (EU, endotoxin units). Quantification of immunoblotting of p-NF-kB p65 normalized to total NF-kB p65. (F) Relative mRNA expression levels of Tnf-α and IL-1β mRNA in RAW264.7 cells (n=5 per group). mRNA expression levels were calculated as the ratio relative to that in WT cells. Results are presented as the mean ± SE. *P<0.05, significantly different from the WT group; †P<0.05, significantly different from the Nrf2-KO group; §P<0.05, significantly different from the p62-KO group.

LPS polarizes macrophages towards the M1 phenotype. M1 macrophages are implicated in the initiation and maintenance of inflammation. To directly test the inflammatory response of Kupffer cells, Kupffer cells from WT, Nrf2-KO, p62-KO, and DKO mice at 8 weeks of age were isolated and stimulated with LPS ex vivo. Analysis using qRT-PCR revealed that upregulation of Tnf-α mRNA with vehicle treatment was only present in DKO mice, indicating innate immunity activation at an early age, and that the magnitude of the upregulation was further potentiated by LPS treatment (data not shown). There was no significant difference in the number of F4/80-positive cells, which is a surface marker of Kupffer cells (Supplementary Fig. 4).

Impairment of the phagocytic function of Kupffer cells may play a critical role in the development of NASH [39, 44]. Therefore, the effects in the liver parenchymal phase were determined by SPIO-MRI, and Kupffer cell phagocytic activity was assessed in mice. The magnitude of changes in the T2 value of the liver parenchymal phase, which reflects the Kupffer cell phagocytic status of SPIO, was smaller in Nrf2-KO, p62-KO, and DKO mice than in WT mice (Fig. 5B). Simply put, the phagocytic ability decreased in these mice. When the ability of F4/80-positive cells to uptake latex beads was investigated by flow cytometry, the bead phagocytic ability was lower in Nrf2-KO and DKO mice than in WT and p62-KO mice (Fig. 5C). Correlating with these findings, the positive fraction of MARCO, a scavenger receptor, decreased in Nrf2-KO and DKO mice. There was no significant difference observed for the positive fraction of SR-A (Fig. 5C).

As noted, macrophages polarizing towards the M1 phenotype and innate immunity were activated in the livers of DKO mice at 8 weeks of age. The findings suggest that the Kupffer cells of DKO mice due to their genetic background accelerate the inflammatory response to excessive LPS flux through an increase in the susceptibility of innate immunity to LPS. We produced RAW264.7 lacking the p62 or Nrf2 gene using the CRISPR-Cas9 system. In vitro experiments were conducted to evaluate the inflammatory response to LPS. Expression of p62 and Nrf2 protein in each gene-deficient cell was confirmed by immunoblot analysis (Fig. 5D). Analysis showed that phosphorylation of Nuclear factor-kappa B p65 (NF-κB p65) increased in Nrf2-deficient cells compared with WT cells (Fig. 5E). Interestingly, Tnf-α and IL-1β mRNA expression levels following treatment with low-dose LPS were also upregulated in Nrf2-deficient cells compared with WT cells (Fig. 5F).

Diet restriction improved NASH in DKO mice

To confirm the association between hyperphagia-induced obesity and NASH development in DKO mice, diet restriction by pair-feeding was conducted using DKO mice. Pair-feeding restricted the intake of standard chow to 3.0 ± 0.1 g/day/mouse. DKO mice subjected to diet restriction showed a body weight gain curve similar to WT mice fed ad libitum (Fig. 6A). When compared with ad libitum DKO mice with liver steatosis, inflammation, and fibrosis, pair-feed DKO mice showed dramatic improvements in these pathological conditions in the liver (Figs. 6B and C). Improvements in NASH conditions were coupled with recovered intestinal permeability (Fig. 6D) and improved microbiota composition (e.g., a decreased proportion of gram-negative bacteria and decreased fecal LPS concentrations) (Fig. 6E) and thereby decreased serum LPS concentrations (Fig. 6F).

Fig. 6.

Fig. 6.

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Diet restriction improved nonalcoholic steatohepatitis (NASH) in p62:Nrf2 gene double-knockout (DKO) mice. (A) Body weight changes in wild-type (WT), ad libitum DKO, and pair-feeding mice (n=10–15 per group). (B) Hematoxylin and eosin (H&E)- and sirius red-stained sections of representative liver specimens from the ad libitum DKO and pair-feeding groups at 30 weeks of age (scale bar, 100 µm). (C) The steatosis, activity, and fibrosis (SAF) scores for each group are shown (8 per group). (D) Area under the plasma concentration curve of fluorescein isothiocyanate (FITC)-dextran in plasma after administration of the marker by gavage (n=8 per group). (E) The relative abundance of gram-negative bacteria was calculated as the percentage of bacteria relative to the total bacteria for fecal bacteria (n=8 per group). (F) Fecal and serum lipopolysaccharide (LPS) levels in WT, ad libitum DKO, and pair-feeding mice (n=8 per group). Results are presented as the mean ± SE. *P<0.05, significantly different from the ad libitum DKO group.

Discussion

The major findings of this study are that p62 deficiency resulted in hyperphagia-induced obesity coupled with insulin resistance and adipokine imbalance as well as dysbiosis and that Nrf2 deficiency resulted in intestinal barrier dysfunction and an accelerated inflammatory response to LPS in Kupffer cells. Both defects resulted in the onset and progression of NASH and were associated with hepatic tumorigenesis in DKO mice fed a normal diet. However, p62 or Nrf2 single gene-knockout mice did not progress to NASH.

Of note, in DKO mice, a high-fat diet is not needed for the development of NASH. In this regard, inflammatory events may lead to fat accumulation (steatosis) in the livers [26]. As proposed by the multiple parallel hits theory, which is currently accepted as the pathogenesis of nonalcoholic fatty liver disease (NAFLD) [38], inflammatory mediators from the intestines (e.g., LPS) and adipose tissue (e.g., adipokines) could play a central role in the cascade of hepatic inflammation and fibrosis development. It is possible that, in DKO mice, activation of innate immunity by an excessive LPS flux, occurring both within and outside the liver, is a central pathway that regulates the development of hepatic damage in the form of NASH.

Cumulative data suggests that dysbiosis of the intestinal microbiota is associated with the onset and progression of NASH [9]. Several studies have reported that serum LPS levels are elevated in NAFLD patients compared with controls [14, 25]. The fecal microbiota in NAFLD or NASH patients has been assessed using culture-independent techniques such as sequencing of a conserved region in the bacterial 16S rRNA gene. Microbiota samples from patients with NAFLD or NASH have a higher proportion of members of the Porphyromonadaceae family and a lower proportion of members of the Lachnospiraceae and Ruminococcaceae families than those from healthy people [42, 47]. Flora analysis of DKO mice in the current study showed an increased proportion of Porphyromonadaceae and decreased proportions of Lachnospiraceae and Ruminococcaceae (Fig. 3). Because we compared proportions of microflora, it is impossible to accurately compare the number of bacteria. The fecal LPS concentration was elevated in both p62-KO and DKO mice. However, serum LPS concentrations were elevated only in DKO mice. Interestingly, diet restriction by pair-feeding decreased fecal and serum LPS concentrations in DKO mice, which in turns resulted in an improvement in the hepatic conditions of NASH (Fig. 6). Moreover, administration of the probiotic VSL#3 from 10 to 25 weeks of age increased the bacterial species Bifidobacterium infantis and Streptococcus thermophilus in feces and reduced serum LPS concentrations (Supplemental Figs. 5A and 5B). In addition, steatosis, inflammation, and fibrosis were improved in the liver (Supplemental Fig. 5C). From these results, it is suggested that p62 deficiency in DKO mice modifies the intestinal flora (e.g., increasing the proportion of gram-negative bacteria) through hyperphagia and thereby increases fecal LPS concentrations. In addition, the activation of TLR4 by LPS from intestinal Gram-negative bacteria has recently been shown to promote the development of hepatocellular carcinoma [6]. Therefore, it is likely that in DKO mice LPS is closely involved in the liver tumorigenesis, since the expression levels of genes downstream of TLR4 were increased in the liver (Table 1).

Previous studies suggest a pathological link between intestinal permeability status and NASH. It is reported that the intestinal microbiome is perturbed in both people with obesity and NAFLD patients, which in turn may lead to increased fecal LPS levels and result in leaky gut [41]. Luca et al. reported that intestinal permeability is increased in patients with NAFLD and that the staining intensity of Zo-1 in the intestinal epithelia significantly decreases in patients with NAFLD [30]. The results of the current study (Fig. 4) indicate that both Nrf2 deficiency in mice and Nrf2 deletion in Caco-2 cells contribute to increased intestinal permeability through a decrease in Zo-1 expression levels, suggesting that Nrf2 is closely involved in the maintenance of intestinal barrier function. In addition, Nrf2 is known to inhibit the expression levels of i-nitric oxide synthase [27]. Therefore, in the damaged intestines of DKO mice (Fig. 4), an LPS-induced increase in inducible NO synthase activity may also be an important factor responsible for the increased permeability as reported previously [11].

Obesity is an inflammatory condition characterized by increased activity of the innate immune system. Increased sensitivity of the liver to LPS in people with obesity is closely associated with the pathogenesis of NASH [43]. Understanding the role of Kupffer cells in the context of inflammation in the pathogenetic process of LPS-induced liver injury has recently increased. Imajo et al. demonstrated that obesity-associated increases in leptin levels result in an increased sensitivity of Kupffer cells to low doses of LPS through increased expression of CD14, a receptor for LPS and LPS-binding protein complexes [16]. In the current study, increased leptin levels in DKO mice at 8 and 30 weeks of age were associated with increased expression levels of pro-inflammatory cytokines in the liver at 8 and 30 weeks of age and those in visceral fat at 30 weeks of age (Table 1).

Activation of innate immunity may play a role in the pathophysiology of inflammation-induced liver damage. Kupffer cells play an important role in innate immunity. Macrophages (Kupffer cells) undergo specific differentiation depending on the local tissue environment, responding to environmental cues within tissues such as damaged cells or microbial products to differentiate into distinct functional phenotypes. In the current study, phenotypic changes in Kupffer cells were observed in DKO mice (Fig. 5), with the proportion of M1 phenotype Kupffer cells increasing. The M1 macrophage phenotype, which is characterized by the production of high levels of pro-inflammatory cytokines and high production of reactive nitrogen and oxygen intermediates, is implicated in initiation and maintenance of inflammation. Therefore, the phenotypic changes are thought to worsen the hepatic conditions of NASH [1].

The responsiveness of cultured macrophages to low doses of LPS increased with Nrf2 deletion in the current study (Fig. 5). Activation of Nrf2 prevents LPS-induced upregulation of pro-inflammatory cytokines [22]. Therefore, an increased inflammatory response to low doses of LPS in Nrf2 deficiency and hyperleptinemia in p62 deficiency are closely associated with maintenance of inflammation in the livers of DKO mice.

In this study, the phagocytic ability of Kupffer cells decreased in DKO mice. Flow cytometry revealed that Nrf2 deficiency reduced the expression level of MARCO as a scavenger receptor and decreased the phagocytic ability of Kupffer cells (Fig. 5). In humans and rats, impaired phagocytic functions of Kupffer cells are causatively associated with the development of NASH [39]. MARCO restricts TLR4 responses to LPS, and expression levels of MARCO increase when LPS is administered [31]. Therefore, decreased expression of MARCO in DKO mice may increase the TLR4 response to low doses of LPS.

Several studies have reported the involvement of p62 and NRF2 in human NASH. p62 is a component of Mallory-Denk bodies, which are protein aggregates that accumulate in damaged liver cells in NASH, cirrhosis, and HCC [36]. Impaired autophagic flux is associated with increased endoplasmic reticulum stress during the development of NASH, and consequently, levels of hepatic p62 protein are significantly increased in patients with NASH compared with those in control subjects [10]. In contrast, Duran et al. showed that p62 expression was significantly lower in tumoral hepatic stellate cells, causing impaired repression of fibrosis and inflammation [8]. Activation of NRF2 is also observed in human NASH [13, 21], indicating that NRF2 plays an important role in adaptation to oxidative stress in NASH and tumors. These reports indicate that p62 and NRF2 may have important roles in human NASH progression.

In conclusion, feeding of a standard diet to DKO mice resulted in the development of NASH and was associated with tumorigenesis. In vivo and in vitro studies revealed that p62 deficiency resulted in hyperphagia-induced obesity coupled with insulin resistance and adipokine imbalance as well as dysbiosis (e.g., an increased proportion of gram-negative bacteria) and that Nrf2 deficiency resulted in intestinal barrier dysfunction and an accelerated inflammatory response to LPS in Kupffer cells. In mice, activation of innate immunity by excessive LPS flux, occurring both within and outside the liver (e.g., intestines and visceral adipose tissue), is central to the development of hepatic damage in the form of NASH (Fig. 7). Because of the phenotypic similarities to the clinical features of human NASH, e.g., obesity (visceral fat accumulation) coupled with metabolic syndrome, insulin resistance, and adipokine imbalance, DKO mice represent a unique animal model for investigating the pathogenesis of human NASH and exploring novel targets for the prevention and/or treatment of NASH. A therapeutic approach targeting the intestinal flora and intestinal barrier function with probiotics may be useful for the potential management of NASH.

Fig. 7.

Fig. 7.

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Summary of nonalcoholic steatohepatitis in p62:Nrf2 double-knockout (DKO) mice.

Author Contributions

K.A., E.W., K.O., and J.S. developed the study concept and experimental design. K.A. performed all animal study procedures and most in vitro experiments. K.T. scored mouse livers for the features of NASH. T.Y., T.I., and K.K. helped with the design and interpreting the results. K.A., E.W., K.O., and J.S. interpreted the data and wrote the manuscript.

Conflict of Interest

The authors declare that they have no potential conflicts of interest.

Supplementary Material

Supplement figure and table
expanim-67-201-s001.pdf (10.8MB, pdf)

Acknowledgments

This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology, Japan (No. 25282212, 26282191, 26293284, 26293297, 15H04917, 15K15037, 15K15488, 16J00793, 16K15188, 17H02174, 17K19887, and 17K19888), and a Grant-in-Aid from the Nakayama Cancer Research Institute (Tokyo, Japan). We would like to thank all our co-workers who supported this work at our institution. We would also like to thank Naohiro Gotoh (Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Tokyo, Japan), Toru Kawamoto (Faculty of Medicine, Tokyo Women’s Medical University, Tokyo, Japan), Tetsuya Ueda (Drug Development Service Division, Pharmacodynamics Group, Medi-chem Business Segment, LSI Medience Corporation, Tokyo, Japan), Tomoyuki Haishi (MRTechnology, Inc., Ibaraki, Japan), and Masayuki Yamamoto (Department of Medical Biochemistry, Tohoku University Graduate School of Medicine, Miyagi, Japan).

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