Abstract
During RNA virus infection, the adaptor protein MAVS recruits TRAF3 and TRAF6 to form a signalosome, which is critical to induce the production of type I interferons (IFNs) and proinflammatory cytokines. While activation of the MAVS/TRAF3/TRAF6 signalosome is well studied, the negative regulation of the signalosome remains largely unknown. Here we report that RNA viruses specifically promote the deubiquitinase OTUD1 expression by NF-κB-dependent mechanisms at the early stage of viral infection. Furthermore, OTUD1 upregulates protein levels of intracellular Smurf1 by removing Smurf1 ubiquitination. Importantly, RNA virus infection promotes the binding of Smurf1 to MAVS, TRAF3 and TRAF6, which leads to ubiquitination-dependent degradation of every component of the MAVS/TRAF3/TRAF6 signalosome and subsequent potent inhibition of IFNs production. Consistently, OTUD1-deficient mice produce more antiviral cytokines and are more resistant to RNA virus infection. Our findings reveal a novel immune evasion mechanism exploited by RNA viruses, and elucidate a negative feedback loop of MAVS/TRAF3/TRAF6 signaling mediated by the OTUD1-Smurf1 axis during RNA virus infection.
Author summary
Extensive studies have demonstrated redundant roles of multiple antiviral signaling pathways in host defense against viral infection. However, the strategies viruses have evolved to efficiently antagonize the complementary effect of alternative IFNs-inducing signaling remain largely unknown. Here we report that RNA viruses specifically promote the expression of the deubiquitinase OTUD1. RNA viruses-induced OTUD1 upregulates intracellular Smurf1 protein levels by deubiquitination modification, and promotes the binding of Smurf1 to MAVS, TRAF3 and TRAF6, which leads to degradation of every component of the MAVS/TRAF3/TRAF6 signalosome and inhibition of IFNs production. Our finding identifies a potent negative regulation of innate antiviral response, which could be crucial for RNA viruses to establish efficient infection at the early stage.
Introduction
The innate immune response is the first line of host defense against viral infection. Viral nucleic acids can be recognized by host pattern recognition receptors (PRRs), including RIG-I-like receptors (RLRs), Toll-like receptors (TLRs) and cytosolic dsDNA sensors (STING) [1–5]. PRRs trigger antiviral signaling and result in the production of type I interferons (IFNs) and proinflammatory cytokines, which are central to the efficient host defense against viral infection [3,4,6]. RLRs (including RIG-I and MDA5) are mainly responsible for the recognition of cytosolic RNAs from RNA viruses [5,7]. RLRs recognize viral RNAs through the RNA helicase domain (RLD), and then interact with the mitochondrial antiviral signaling protein MAVS [7,8]. MAVS further recruits the tumor necrosis factor receptor-associated factor (TRAF) family proteins to form a key signaling platform, which triggers the IFNs antiviral response through activation of two important signaling pathways, type I IFNs (IFNα/β)-mediated antiviral IFN-stimulated genes (ISGs) signaling and NF-κB-mediated proinflammatory signaling [9–14].
MAVS is also known as IPS-1, Cardif or VISA [7,8,15,16]. Upon binding with viral RNAs, RIG-I activates MAVS by promoting MAVS polymerization on the mitochondrial surface [17,18]. MAVS polymers, as the central platform, recruit both TRAF3 and TRAF6 to form the MAVS/TRAF3/TRAF6 signalosome, which is essential to activate downstream antiviral signaling [18–21]. The MAVS/TRAF3/TRAF6 signalosome either interacts with a complex containing TBK1 and IKK to activate the transcription factor IRF3 and subsequent type I IFNs production, or interacts with IKKα/β/γ complex to activate NF-κB and produce downstream proinflammatory cytokines [18]. TRAF3 and TRAF6 belong to the TRAF family of ring-finger ubiquitin E3 ligases. TRAF3 regulates viral infection-triggered induction of IFNβ [22,23]. However, it was reported that in TRAF3-deficient MEFs RNA viruses can normally activate IRF3 and induce a modestly reduced level of IFNβ [18,24], suggesting that the function of TRAF3 can be substituted by other antiviral signaling for IFNs induction during viral infection. Interestingly, TRAF6 was also able to activate IRF3 and induce IFNs during viral infection [18]. Moreover, TRAF6 can act redundantly with TRAF2/5 to promote IRF3 and IKK activation by more complex mechanisms [18]. These studies indicate that TRAF family members (e.g. TRAF3 and TRAF6) could play redundant roles in inducing IFNs antiviral responses. However, whether and how viruses have evolved strategies to efficiently antagonize the complementary effect of various IFNs antiviral signaling remain largely unexplored.
Recently, some studies reported that negative regulation of MAVS levels by ubiquitin-proteasome system results in the inhibition of antiviral signaling. For example, PCBP1 and PCBP2 recruit Itch to induce K48-linked ubiquitination and degradation of MAVS [25,26]. In addition, viruses have evolved some strategies to antagonize MAVS-mediated antiviral signaling. Hepatitis B virus can induce ubiquitination on lysine 136 and degradation of MAVS through its X protein [27]. However, the deubiquitinases that regulate ubiquitination and levels of MAVS remain unexplored. Thus far, several ubiquitin E3 ligases and deubiquitinases have been reported to be associated with the activity or stability of TRAF3 and TRAF6. Cellular apoptosis inhibitors cIAP1 and cIAP2 induce K63-linked ubiquitination of TRAF3 and promote virus-triggered activation of NF-κB and IRF3 [28]. The deubiquitinases OTUB1/2 [29,30], UCHL1 [31], MYSM1 [32] and DUBA [33] inhibit K63-linked ubiquitination of TRAF3 or TRAF6 and negatively regulate IFNs production during viral infection. USP25 has been shown to enhance the stability of TRAF3 and TRAF6 [34]. However, how viral infection induces some common mechanisms to negatively regulate the stability of MAVS/TRAF3/TRAF6 signalosome remains to be illuminated.
In the present study, we report that the OTU deubiquitinase 1 (OTUD1) negatively regulates RNA virus-triggered production of type I IFNs and proinflammatory cytokines. OTUD1-deficient mice are more resistant to RNA virus infection by producing more type I IFNs. We further uncover that RNA viruses, but not DNA viruses, promote Otud1 expression through the NF-κB signaling pathway. And OTUD1 upregulates Smurf1 protein levels by removing ubiquitination of Smurf1. Interestingly, RNA virus infection promotes the binding of Smurf1 to MAVS, TRAF3 and TRAF6, which results in ubiquitination and proteasome-dependent degradation of every component of the MAVS/TRAF3/TRAF6 signalosome. Our findings reveal a potent negative regulation of innate antiviral immune response by the OTUD1-Smurf1 axis-mediated downregulation of the MAVS/TRAF3/TRAF6 signalosome.
Results
OTUD1 is involved in the regulation of RNA virus-induced production of type I IFNs and proinflammatory factors
In a screen of deubiquitinases expression cloning library [35,36], we found that the deubiquitinase OTUD1 could inhibit RNA virus-induced production of type I IFNs. To further study the effect of OTUD1 on type I IFNs production, endogenous OTUD1 was knocked down and IFNβ mRNA levels after Sendai virus (SeV) infection were analyzed. The result showed that knockdown of OTUD1 promoted SeV-induced IFNβ production (Fig 1A and S1A Fig). In accordance with this response, the activity of promoters containing IFNs-stimulated response element (ISRE) was enhanced by OTUD1 knockdown (S1B Fig). And exogenous expression of OTUD1 lowered SeV-induced ISRE promoter activity (S1C Fig). To confirm the role of OTUD1 in the regulation of the production of type I IFNs, we infected mouse embryonic fibroblasts (MEFs) from Otud1+/+ and Otud1-/- mice with either RNA viruses including SeV, vesicular stomatitis virus (VSV) and influenza A virus H1N1 (PR/8/34), or DNA viruses herpes simplex virus (HSV). The levels of IFNβ mRNA in Otud1-/- MEFs were much higher than that in Otud1+/+ MEFs during infection of SeV, or VSV (Fig 1B), or H1N1 (S1D Fig). Interestingly, OTUD1 deletion did not affect production of IFNβ mRNA induced by HSV infection (Fig 1B). Given that HSV, as a DNA virus, majorly activates the STING (stimulator of interferon genes) signaling pathway, we employed another two specific stimulators, ISD (interferon stimulatory DNA) and cGAMP, for the DNA-STING signaling. The result showed that OTUD1 deficiency did not significantly affect IFNβ production stimulated by both ISD and cGAMP (Fig 1C). Together, these findings indicate that OTUD1 is involved in IFNs induction by RNA viruses, but not DNA-STING signaling. Interestingly, when Otud1+/+ and Otud1-/- MEFs were stimulated by either Poly(I:C) (a TLR3 activator) or LPS (lipopolysaccharide, a TLR4 activator), we found that IFNβ induction by either Poly(I:C) or LPS was remarkably upregulated in Otud1-/- cells (S1E Fig), indicating that OTUD1 could play roles in IFNs induction by at least some of TLRs signaling.
Consistent with the data from RNA viruses, Otud1-/- mice had more IFNβ protein in the sera than did Otud1+/+ mice in response to VSV infection (S1F Fig). Additionally, Otud1-/- MEFs displayed more expression of interferon-stimulated genes (ISGs) including IFIT1, ISG15 and ISG54 during SeV infection, as compared with their wild-type counterparts (Fig 1D). Similarly, knockdown of OTUD1 by shRNAs promoted SeV-induced mRNA expression of ISGs (S1G Fig). Interestingly, OTUD1 deficiency also promoted RNA virus-induced production of proinflammatory factors such as TNFα and IL-6 in either MEFs (Fig 1E) or mouse primary liver cells (S1H and S1I Fig), suggesting that OTUD1 could target certain components upstream of RLRs-mediated antiviral signaling. Finally, we analyzed the effect of OTUD1 deficiency on viral infection. The result showed that OTUD1 deletion markedly downregulated viral RNA levels in MEFs cells infected with both SeV and VSV (Fig 1F). However, OTUD1 deficiency did not affect HSV RNA levels (Fig 1F). Furthermore, viral titers were determined by the 50% tissue culture infectious dose (TCID50) assay. We found that OTUD1 deletion lowered RNA virus VSV viral titers (Fig 1G and 1I), but did not downregulate HSV viral titers (Fig 1H). Taken together, these results suggest that OTUD1 is involved in host defense against RNA viruses.
OTUD1 deficiency protects mice from RNA virus infection
To investigate the role and functional importance of OTUD1 in host antiviral response in vivo, we challenged wild-type and Otud1-/- mice with VSV using intraperitoneal injection. At day 3 after VSV infection, Otud1-/- mice produced much higher expression of IFNβ and IL-6 mRNAs (Fig 2A), and had much lower viral RNAs (Fig 2A) in their lung tissue than did Otud1+/+ mice. Consistent with the results obtained by intraperitoneal VSV injection, Otud1-/- mice with VSV intranasal infection had lower VSV loads at day 3 than did Otud1+/+ mice (Fig 2B). Furthermore, when viral infection was extended to 14 days, VSV loads in different organs from Otud1-/- mice were significantly reduced compared to their wild-type counterparts (Fig 2C). Then we observed the expression of proinflammatory factor IL-6 mRNA in different organs from wild-type and Otud1-/- mice. We found that the levels of IL-6 mRNA in organs from Otud1-/- mice were much higher than that from Otud1+/+ mice (Fig 2D). We next challenged Otud1+/+ and Otud1-/- mice with VSV and monitored their survival. The results showed that Otud1-/- mice were more resistant to VSV infection in overall survival assays (Fig 2E). To directly observe the VSV infection in different organs from wild-type and Otud1-/- mice, immunohistochemical staining for VSV encoded protein VSVG was carried out. The results showed less VSV staining in lung, kidney and liver tissue from Otud1-/- mice, as compared with wild-type mice (Fig 2F). Collectively, our data suggest that Otud1-/- mice possess more potent host defense against RNA viruses by promoting the induction of type I IFNs and proinflammatory cytokines.
OTUD1 negatively regulates protein levels of MAVS, TRAF3, and TRAF6
To uncover the mechanisms by which OTUD1 regulates antiviral innate immune response, we first analyzed the effect of OTUD1 on activation of IFNβ promoter during RNA virus infection. Results showed that knockdown of OTUD1 significantly enhanced activation of IFNβ promoter during SeV infection (S2A Fig), and overexpression of OTUD1 inhibited SeV-induced activation of IFNβ promoter (S2B Fig). Consistently, OTUD1 knockdown increased phosphorylated IRF3 levels induced by SeV infection (Fig 3A). Conversely, OTUD1 overexpression decreased the levels of phosphorylated IRF3 (S2C Fig) and IRF3 homodimers (S2D Fig) during viral infection. To further determine at what level in RLRs-mediated antiviral signaling pathway OTUD1 blocked activation of IFNβ promoter, three expression constructs (RIG-I, MAVS, or TBK1) were overexpressed in cells in the presence or absence of OTUD1. The results showed that OTUD1 overexpression dramatically inhibited IFNβ promoter activation driven by both RIG-I and MAVS (Fig 3B), whereas IFNβ promoter activation by TBK1 (Fig 3B) was not inhibited by OTUD1 coexpression. These data indicate that OTUD1 inhibits the IFNs antiviral response downstream of MAVS and upstream of TBK1.
To further identify the signaling proteins targeted by OTUD1, the interaction between OTUD1 and MAVS was first analyzed. Our data showed that OTUD1 was able to interact with MAVS (S3A Fig). MAVS is a key platform protein that majorly recruits TRAF3 and TRAF6 to form a signalosome. Therefore, we studied whether OTUD1 could interact with TRAF3 or TRAF6. Results showed that OTUD1 was also capable of interacting with both TRAF3 (S3B Fig) and TRAF6 (S3C Fig). Interestingly, we repeatedly observed that protein levels of MAVS (S3A and S4A Figs), TRAF3 (S3B and S4B Figs), and TRAF6 (S3C and S4C Figs) were noticeably decreased when OTUD1 was coexpressed. These data raise the question whether OTUD1 could regulate MAVS/TRAF3/TRAF6 signaling. To address this hypothesis, OTUD1 was gradually overexpressed in cells and the levels of endogenous signaling proteins in the antiviral signaling pathway were firstly determined. Our data showed that OTUD1 overexpression had no obvious effects on IRF3 and TBK1 protein levels, and increased RIG-I protein levels to some extent (Fig 3C). However, we noticed that OTUD1 overexpression gradually downregulated the levels of MAVS, TRAF3, and TRAF6 (Fig 3C), which is consistent with the observation that OTUD1 overexpression inhibits the induction of type I IFNs and proinflammatory cytokines. To confirm the effects of OTUD1 on MAVS/TRAF3/TRAF6 protein levels, we determined the levels of endogenous MAVS/TRAF3/TRAF6 in Otud1+/+ and Otud1-/- MEFs. The results showed that cellular levels of MAVS, TRAF3, and TRAF6 in Otud1-/- MEFs were higher than that in wild-type counterparts to some extent (Fig 3D). Together, these data suggest that OTUD1 negatively regulates protein levels of MAVS, TRAF3, and TRAF6.
Next, we hypothesize that OTUD1 affects protein stability of MAVS, TRAF3, and TRAF6. Endogenous MAVS, or TRAF3, or TRAF6 proteins from Otud1+/+ and Otud1-/- MEFs were immunoprecipitated, and the ubiquitination levels were firstly determined by a specific ubiquitin antibody. We found that the ubiquitination levels of MAVS (Fig 3E), TRAF3 (Fig 3F), and TRAF6 (Fig 3G) were reduced in Otud1-/- MEFs when comparing to Otud1+/+ MEFs. And overexpression of OTUD1 promoted K48-linked ubiquitination of MAVS/TRAF3/TRAF6 (S4E Fig), suggesting that OTUD1 promotes ubiquitination and degradation of MAVS/TRAF3/TRAF6. Furthermore, the proteasome inhibitor MG132 was used to block ubiquitin-proteasome degradation. We noticed that endogenous MAVS protein is relatively stable, which is consistent with endogenous OTUD1 protein stability, as shown by our observation that the pretreatment of cells with MG132 for 8 hr and 12 hr did not significantly upregulate protein levels of endogenous OTUD1 and MAVS (S4F Fig). However, when OTUD1 was overexpressed, the levels of MAVS were markedly downregulated (Fig 3H). Similarly, OTUD1 overexpression lowered protein levels of TRAF3 (Fig 3I) and TRAF6 (Fig 3J). In the presence of MG132, the ability of OTUD1 to downregulate MAVS (Fig 3H), or TRAF3 (Fig 3I), or TRAF6 (Fig 3J) was largely inhibited. Collectively, these results suggest that OTUD1 plays a role in promoting proteasome-dependent degradation of MAVS/TRAF3/TRAF6.
Both Smurf1 and the deubiquitinase activity of OTUD1 are required for OTUD1-mediated downregulation of MAVS/TRAF3/TRAF6 and inhibition of interferon antiviral response
OTUD1 is the member of the deubiquitinases, which are supposed to remove ubiquitination from protein substrates and upregulate protein levels. Our above data demonstrated that OTUD1 promoted ubiquitination of MAVS/TRAF3/TRAF6, and downregulated their protein levels, suggesting that MAVS, TRAF3, and TRAF6 are not the direct targets of OTUD1 as a deubiquitinase. Therefore, we asked whether OTUD1 could upregulate certain ubiquitin E3 ligases, which directly regulate ubiquitination and degradation of MAVS/TRAF3/TRAF6. So far, several ubiquitin E3 ligases have been reported to be involved in promoting TRAF3/6 ubiquitination. Both cIAP1 and cIAP2 promoted only K63-linked ubiquitination of TRAF3 [28], which should not be the ubiquitin E3 ligase resulting in TRAF3 downregulation. It was reported that Ndfip1 interacted with the E3 ligase Smurf1 and promoted MAVS degradation [37]. In addition, the E3 ligase Smurf2 also induced ubiquitination and degradation of MAVS [38]. We further found that OTUD1 overexpression upregulated protein level of Smurf1, rather than Smurf2 (S4D Fig). Therefore, we tried to analyze whether Smurf1 could be required for OTUD1-mediated downregulation of MAVS. Our data showed that knockdown of Smurf1 significantly inhibited OTUD1-mediated MAVS downregulation (Fig 4A). Interestingly, Smurf1 knockdown also remarkably blocked OTUD1-mediated downregulation of both TRAF3 (Fig 4B) and TRAF6 (Fig 4C). Consistently, knockdown of Smurf1 inhibited OTUD1-induced ubiquitination of MAVS (Fig 4D), TRAF3 (Fig 4E) and TRAF6 (Fig 4F). The role of Smurf1 in regulating TRAF3/TRAF6 protein levels during viral infection remains unknown so far, although Smurf1 was reported to be able to bind to and ubiquitinate exogenous Myc-TRAFs family members in HEK293T cells [39]. Here, our results indicate that OTUD1 could utilize Smurf1 to downregulate MAVS/TRAF3/TRAF6 proteins and restrict IFNs production during viral infection. Therefore, we further observed the role of Smurf1 in OTUD1-mediated inhibitory effect on IFNs production during viral infection. Our data showed that overexpression of OTUD1 inhibited the production of IFNβ in response to SeV infection, whereas knockdown of Smurf1 largely blocked the negative regulation of OTUD1 on IFNβ production (Fig 4G), indicating that Smurf1 is required for OTUD1-mediated inhibition of interferon antiviral response.
We next investigate whether the deubiquitinase activity of OTUD1 is required for downregulation of MAVS/TRAF3/TRAF6 and interferon antiviral response. According to the conservative sites of the deubiquitinase activity of OTU family [40], we constructed OTUD1-C320A-H431Q (CH) mutant. As compared with OTUD1-wild type constructs, OTUD1-CH mutants lost the ability to downregulate MAVS, TRAF3, and TRAF6 (Fig 4H). In addition, we observed that OTUD1-CH mutants were unable to upregulate Smurf1 protein as well (Fig 4H). These results indicate that the deubiquitinase activity of OTUD1 is required for Smurf1 upregulation and MAVS/TRAF3/TRAF6 downregulation.
Given that OTUD1 downregulated MAVS/TRAF3/TRAF6 protein levels, we next questioned whether OTUD1 could inhibit MAVS/TRAF3/TRAF6 signalosome-mediated IFNs production and antiviral activity. During infection with SeV, overexpression of OTUD1-wild type obviously lowered the activity of IFNβ promoter, whereas overexpression of OTUD1-CH mutant completely had no effect on the IFNβ promoter activity (Fig 4I), suggesting that the deubiquitinase activity of OTUD1 is important for OTUD1-mediated inhibition of IFNβ production during viral infection. Moreover, we investigated the effect of the deubiquitinase activity of OTUD1 on viral infection. The results showed that overexpression of OTUD1-wild type dramatically promoted VSV infection (Fig 4J). In contrast, overexpression of OTUD1-CH mutant had no effect on VSV infection (Fig 4J). Taken together, these results suggest that both Smurf1 and the deubiquitinase activity of OTUD1 are required for the downregulation of MAVS/TRAF3/TRAF6 signalosome proteins, which results in the OTUD1-mediated inhibition of interferon antiviral response.
OTUD1 regulates ubiquitination and protein levels of Smurf1
The aforementioned results prove that Smurf1 is important for OTUD1-mediated inhibition of interferon antiviral response. We next sought to determine whether OTUD1 could regulate Smurf1. We found that Smurf1 was able to interact with OTUD1 (Fig 5A). Overexpression of OTUD1 upregulated exogenous Smurf1 protein levels in a dose-dependent manner (Fig 5B). When endogenous Smurf1 protein was analyzed in Otud1+/+ and Otud1-/- MEFs, we found that the level of endogenous Smurf1 in Otud1-/- MEFs was noticeably lower than that in Otud1+/+ MEFs (Fig 5C), suggesting that OTUD1 is a positive regulator of Smurf1 protein levels.
Furthermore, the effect of OTUD1 on Smurf1 ubiquitination was determined. The results showed that overexpression of OTUD1 significantly removed polyubiquitination of Smurf1 (Fig 5D). Next, we analyzed the deubiquitination types of Smurf1 mediated by OTUD1 using two ubiquitin mutants, Ub-R48K (all lysines in Ub are mutated to arginines except lysine 48 residue) and Ub-R63K (all lysines in Ub are mutated to arginines except lysine 63 residue). We found that OTUD1 overexpression decreased both K48-linked and K63-linked ubiquitination of Smurf1 (Fig 5E). By a specific K48-linked ubiquitin antibody, we confirmed that OTUD1 was capable of removing K48-linked ubiquitination of Smurf1 (Fig 5F). In addition, the deubiquitination effect of OTUD1 on Smurf1 protein was dependent on the deubiquitinase activity of OTUD1 (Fig 5G). Furthermore, we analyzed ubiquitination of endogenous Smurf1 in Otud1+/+ and Otud1-/- MEFs. The results showed that OTUD1 deficiency upregulated ubiquitination levels of endogenous Smurf1 protein (Fig 5H). Thus, these data demonstrate that OTUD1 positively regulates cellular levels of Smurf1 by deubiquitination effects.
RNA virus infection promotes NF-κB-dependent expression of OTUD1 that upregulates Smurf1 protein levels
We next sought to determine how viral infection affects the levels of OTUD1, Smurf1 and MAVS/TRAF3/TRAF6, and whether the regulation signaling of OTUD1-Smurf1-MAVS/TRAF3/TRAF6 occurs during viral infection. We found that VSV infection upregulated expression of OTUD1 mRNA in human fibrosarcoma cells HT1080 (Fig 6A, left panel) and 2fTGH (S5A Fig). Similarly, SeV infection also promoted expression of OTUD1 mRNA in a time-dependent manner (Fig 6A, middle panel). However, DNA viruses HSV did not upregulate OTUD1 mRNA expression in 2fTGH (Fig 6A, right panel) or MEFs (S5B Fig) at the early stage of infection. Besides HSV, another two stimulators for the STING signaling, ISD and cGAMP, did not activate OTUD1 mRNA expression either (S5D and S5E Fig). Consistently, as demonstrated before, OTUD1 cannot regulate IFNs induction by these three stimulators for the STING signaling (Fig 1B and 1C). Interestingly, we found that Poly(I:C), a TLR3-signaling stimulator, also induced OTUD1 mRNA expression (S5C Fig). Consistently, OTUD1 is able to regulate Poly(I:C)-induced IFNs production (S1E Fig). Based on all above observations, we think that activation of OTUD1 expression is an important trigger for the OTUD1-Smurf1-MAVS/TRAF3/TRAF6-IFNs signaling.
During RNA virus infection, two major activated pathways including IFNs signaling and NF-κB signaling induce activation and expression of thousands of downstream genes. Furthermore, we studied which signaling is responsible for upregulation of OTUD1 mRNA during RNA virus infection. We found that VSV infection also upregulated expression of OTUD1 mRNA in Vero cells (type I interferon-deficiency) and U3A cells (STAT1-deficiency) (Fig 6B). Using a NF-κB inhibitor PDTC, we found that VSV-induced upregulation of OTUD1 mRNA was significantly inhibited by pretreatment of cells with the NF-κB inhibitor (Fig 6C), suggesting that NF-κB signaling contributes to RNA virus-induced upregulation of OTUD1 mRNA. As to Smurf1 mRNA, VSV infection did not significantly affect the level of Smurf1 mRNA in HT1080 and 2fTGH cells within 6 hours after infection (Fig 6D). Collectively, these data suggest that during RNA virus infection OTUD1 mRNA was upregulated via virus-induced NF-κB signaling, whereas the level of Smurf1 mRNA remains relatively stable at the early stage of RNA virus infection.
The above results demonstrated that RNA virus infection did not significantly change the level of Smurf1 mRNA at the early stage. However, we found that SeV infection in HeLa cells obviously upregulated Smurf1 protein levels in a time-dependent manner (S5F Fig). This similar phenomenon of Smurf1 upregulation during RNA virus infection was also observed in other cell lines including MEFs (Fig 6E). Interestingly, there was a positive correlation between Smurf1 and OTUD1 protein levels during RNA virus infection, as shown in MEFs cells (Fig 6E). Moreover, when analyzing the changes of Smurf1 protein levels in Otud1+/+ and Otud1-/- MEFs during SeV infection, we found that Smurf1 protein levels were gradually upregulated in Otud1+/+ MEFs, whereas SeV infection did not upregulate Smurf1 protein levels in Otud1-/- MEFs (Fig 6F). Conversely, in Otud1-/- MEFs Smurf1 protein was quite unstable and was rapidly downregulated during SeV infection (Fig 6F). Taken together, these results suggest that RNA viruses specifically upregulate OTUD1 expression, which increases the stability and levels of Smurf1 protein.
RNA virus infection promotes the interaction between OTUD1 and Smurf1, and binding of Smurf1 to MAVS/TRAF3/TRAF6
Given that RNA viruses activate OTUD1 expression and subsequent Smurf1 upregulation, we further analyze whether OTUD1 interacts with Smurf1, and how Smurf1 is able to affect MAVS/TRAF3/TRAF6 proteins during RNA virus infection. To this end, 2fTGH cells were infected with SeV, and then endogenous OTUD1 protein was immunoprecipitated. We found that SeV infection promoted interaction between OTUD1 and Smurf1 (Fig 7A). Similarly, by Immunofluorescence Confocal assay, we observed that SeV infection obviously promoted protein expression and accumulation of both OTUD1 and Smurf1 in cells, and enhanced co-localization between OTUD1 and Smurf1 (Fig 7B).
Next, we ask whether RNA virus infection is capable of inducing interaction between Smurf1 and MAVS/TRAF3/TRAF6. If so, where does Smurf1 interact with MAVS/TRAF3/TRAF6 in response to RNA viruses? Our results clearly showed that SeV infection significantly promoted the binding of Smurf1 to MAVS/TRAF3/TRAF6 proteins in BMDMs (Fig 7C) and 2fTGH cells (S6A Fig). Given that MAVS is a mitochondrial protein and Smurf1 can regulate MAVS/TRAF3/TRAF6 proteins during RNA virus infection, we speculate that upregulated Smurf1 proteins by viral infection could be able to localize to the mitochondria to interact with the MAVS/TRAF3/TRAF6 signalosome. Our data showed that some of Smurf1 proteins localized to the mitochondria in cells infected with SeV for 8 hr (Fig 7D), supporting the role of Smurf1 in regulating the MAVS/TRAF3/TRAF6 signalosome on the mitochondria. Interestingly, we did not observe obvious co-localization between OTUD1 and the mitochondria during SeV infection (Fig 7E), which is also consistent with the observation that OTUD1 does not interact with MAVS, TRAF3, or TRAF6 in cells infected with SeV (Fig 7A). Taken together, we think that RNA virus infection activates OTUD1 expression, which, as a trigger factor, promotes accumulation and activation of Smurf1 protein by interacting with and deubiquitinating Smurf1. Then the activated Smurf1 proteins localize to the mitochondria to interact with the MAVS/TRAF3/TRAF6 signalosome. Finally, Smurf1 promotes ubiquitination and degradation of MAVS/TRAF3/TRAF6 proteins and inhibition of IFNs production.
RNA virus infection utilizes the OTUD1-Smurf1 axis to promote downregulation of MAVS/TRAF3/TRAF6 proteins and IFNs production
Furthermore, the protein and mRNA levels of MAVS, TRAF3, and TRAF6 during RNA virus infection were observed. We noticed that some studies have shown that protein levels of MAVS, or TRAF3, or TRAF6 were downregulated to some extent after infection with different viruses [34,41–43]. Similarly, we repeatedly observed the downregulation of MAVS/TRAF3/TRAF6 protein levels within 12 hr after RNA virus infection in all tested types of cells including HepG2 (Fig 8A), HeLa (Fig 8F), and BMDMs (Fig 8G). Given that RNA virus infection downregulated MAVS/TRAF3/TRAF6 proteins, we want to know how the induction of IFNs mRNA is regulated during this stage. The results showed that the level of IFNβ mRNA was gradually reduced 8–12 hr after SeV infection (Fig 8B), which is consistent with the downregulation of the MAVS/TRAF3/TRAF6 signalosome.
The above data demonstrated that RNA virus infection downregulated MAVS/TRAF3/TRAF6 protein levels, we next questioned whether RNA virus infection downregulates mRNA levels of MAVS, TRAF3, and TRAF6. The results showed that SeV infection had no obvious effect on the levels of both MAVS and TRAF6 mRNAs within 12 hr after infection (Fig 8C and 8E). And TRAF3 mRNA levels were slightly upregulated after 6 hr infection with SeV (Fig 8D). These results suggest that RNA virus infection-induced downregulation of MAVS/TRAF3/TRAF6 proteins cannot be due to the regulation at mRNA level. In conjunction with our previous finding showing that both Smurf1 and the deubiquitinase activity of OTUD1 were required for the downregulation of MAVS/TRAF3/TRAF6 proteins (Fig 4), we first determined whether Smurf1 contributes to RNA virus-induced downregulation of MAVS/TRAF3/TRAF6 signalosome proteins. Moreover, knockdown of Smurf1 markedly inhibited SeV-induced downregulation of MAVS/TRAF3/TRAF6 proteins (Fig 8F), suggesting that Smurf1 contributes to RNA virus-induced downregulation of the MAVS/TRAF3/TRAF6 signalosome proteins.
Furthermore, we analyzed the role of OTUD1 in downregulating MAVS/TRAF3/TRAF6 proteins during RNA virus infection. Our data showed that OTUD1 deficiency in Otud1-/- BMDMs blocked the downregulation of MAVS/TRAF3/TRAF6 proteins during VSV infection, when compared to Otud1+/+ BMDMs (Fig 8G), suggesting that OTUD1 is required for RNA virus-induced downregulation of MAVS/TRAF3/TRAF6 proteins. Moreover, the downregulation course of IFNβ mRNA within 12 hr infection with RNA viruses was blocked in Otud1-/- primary liver cells (S6B Fig), as well as in Otud1-/- MEFs (Fig 8H). Taken together, these data suggest that OTUD1 is responsible for downregulation of both MAVS/TRAF3/TRAF6 signalosome proteins and subsequent production of IFNβ mRNA at the early stage of RNA virus infection.
Discussion
OTUD1 belongs to the ovarian tumor (OTU) family of the deubiquitinases [36]. Until this point, the biological functions of OTUD1 remain unknown. In this study, we uncovered that OTUD1 can downregulate protein levels of MAVS, TRAF3 and TRAF6. Importantly, OTUD1 contributes to RNA virus-induced downregulation of MAVS/TRAF3/TRAF6 proteins. As a consequence, OTUD1 inhibits MAVS/TRAF3/TRAF6 signalosome-mediated production of type I IFNs and proinflammatory cytokines during RNA virus infection (Fig 1). And Otud1-/- mice produce higher levels of type I IFNs and are more resistant to RNA virus infection, when comparing to Otud1+/+ mice (Fig 2). Collectively, these findings reveal for the first time that OTUD1 plays critical roles in regulating host innate antiviral response.
In this study, we reveal that RNA virus infection activates expression of OTUD1, which in turn interacts with Smurf1 and upregulates Smurf1 protein levels by deubiquitination effects. We speculate that accumulation of Smurf1 proteins in cells could result in Smurf1 protein modification (such as phosphorylation, acetylation, ubiquitination, and so on), and subsequent functional activation. As observed in this study, RNA virus infection promotes Smurf1 protein accumulation in cells, and results in re-localization of Smurf1 to the mitochondria. Therefore, we can understand that RNA virus infection promotes binding of Smurf1 to MAVS/TRAF3/TRAF6 proteins, since the MAVS/TRAF3/TRAF6 signalosome is induced on the mitochondria by RNA virus infection.
Our study clearly demonstrates that the deubiquitinase OTUD1 negatively regulates RLR signaling. As a matter of fact, a great number of E3 ubiquitin ligases and deubiquitinases have been reported to be involved in the negative regulation of RLR signaling [44]. However, most of them target RIG-I or MDA5, which is the direct sensor of viral RNA components. Some E3 ubiquitin ligases including RNF125, MARCH5, Smurf2 and AIP4 can target MAVS for K48-linked ubiquitination and degradation [44]. Similarly, it has been reported that some deubiquitinases including OTUB1/2, UCHL1, MYSM1 and DUBA can remove K63-linked ubiquitination of either TRAF3 or TRAF6, thus inhibiting IFNs production during viral infection [29–33]. These reports suggest that MAVS, or TRAF3, or TRAF6 can be delicately regulated by different E3 ligases/deubiquitinases under different infection conditions (for example, different virus types, virus amounts, or different stages of infection). Here, our study uncovers a very different regulation mechanism from these E3 ubiquitin ligases and deubiquitinases. We demonstrate that at the early stage of infection, RNA virus infection rapidly activates OTUD1 expression by inducing OTUD1 mRNA in a NF-κB-dependent manner. OTUD1 upregulation results in increased protein levels and accumulation of Smurf1 by deubiquitination effects, which in turn facilitates Smurf1 localization to the mitochondria, where Smurf1 interacts with and degrades the MAVS/TRAF3/TRAF6 signalosome proteins. Finally, the deubiquitinase OTUD1 potently inhibits RLR pathway by utilizing Smurf1-MAVS/TRAF3/TRAF6 signaling at the early stage of viral infection.
Interestingly, some deubiquitinases have been implicated as positive regulators for RLR signaling. These deubiquitinases can be activated during viral infection, and then stabilize MAVS, or TRAF3, or TRAF6 protein, which finally promotes IFNs production and host antiviral defense. For example, USP25 was reported to be able to enhance the stability of TRAF3 and TRAF6, thus promoting IFNs induction by viral infection [34]. These findings could support our observation that at the late stage (around 20–24 h) of viral infection IFNs production was re-upregulated (Fig 8B), which could result from other positive regulators (for example, some deubiquitinases) of MAVS/TRAF3/TRAF6 proteins. Therefore, we think that at the late stage of viral infection, more signaling molecules take part in the regulation of the MAVS/TRAF3/TRAF6 signalosome, and finally result in the right balance of RLR-MAVS-IFNs signaling.
We clearly showed that during RNA virus infection protein levels of OTUD1 and Smurf1 significantly increased. Given that we have demonstrated that the OTUD1-Smurf1 axis promoted downregulation of MAVS/TRAF3/TRAF6 proteins, we think that the levels of MAVS, TRAF3 and TRAF6 should be decreased at certain time points during RNA virus infection. However, until this point there are few studies that clearly analyzed the dynamics of downregulation of MAVS/TRAF3/TRAF6 proteins during viral infection. It could be partially because most of previous studies focused on the signaling which regulates only one component of this MAVS/TRAF3/TRAF6 signalosome. Here, our findings uncover the phenomenon of MAVS/TRAF3/TRAF6 downregulation at the early stage of viral infection, and partially clarify the mechanisms of the dynamics of downregulation of the signalosome and IFNs induction.
In summary, we elucidate that RNA virus infection upregulates Otud1 expression via the NF-κB signaling pathway. RNA virus-induced OTUD1 protein regulates Smurf1 ubiquitination and increases the level of Smurf1 protein. RNA virus infection also promotes the binding of Smurf1 to MAVS/TRAF3/TRAF6 proteins, thus downregulating MAVS/TRAF3/TRAF6 protein levels by ubiquitination and proteasome-dependent degradation. Consequently, OTUD1 negatively regulates RNA virus-triggered production of type I IFNs and proinflammatory cytokines. This model explains why OTUD1 deficiency protects mice from RNA virus infection. Our findings have revealed a novel negative feedback regulation of innate antiviral immune response, and could provide potential therapeutic targets for viral infection-related diseases.
Materials and methods
Mice
Otud1-/- mice on a C57BL/6 background were generated by Cyagen Biosciences Inc. (Guangzhou, Guangdong, China). Mice were maintained and bred in special-pathogen-free (SPF) conditions in the Experimental Animal Center of Soochow University. All knockout mice were identified via PCR of genomic DNA from tails using the primers: 5’-CTGTGGCGCAGCA CGAATTGGGT-3’ (Forward), 5’-ATGTGCGCCGTGGACGTGAAGT-3’ (Reverse). 6–8 weeks old mice were used in most of experiments.
Ethics statement
Animal care and use protocol adhered to the National Regulations for the Administration of Affairs Concerning Experimental Animals. All protocols and procedures for mice study were performed in accordance with the Laboratory Animal Management Regulations with approval of the Scientific Investigation Board of Soochow University. The project license number is 201705A299.
Cells isolation from mice
Bone marrows were prepared from the 8 weeks adult mice. And the cells were cultured in RPMI medium supplemented with GM-CSF (50 ng/ml) for 7 days for BMDMs differentiation. For the MEFs, Otud1+/+ or Otud1-/- embryos were obtained in the pregnant 13 days mice. The primary mouse liver cells were isolated from mouse liver tissues, which were cut into pieces and digested by erythrocyte lysis buffers. After centrifugation, cells were collected and cultured in PRIM medium and prepared for further experiments.
Viral infection in vitro
Vesicular Stomatitis Virus (VSV) and Sendai virus (SeV) were provided by Dr. Chen Wang (Shanghai Institutes for Biological Science, Chinese Academy of Science, China). Influenza A Virus (H1N1, PR/8/34) was from Dr. Jianfeng Dai (Institutes of Biology and Medical Sciences, Soochow University). Herpes simplex virus (HSV) was from Dr. Chunfu Zheng (Soochow University). Cells were prepared for viral infection to detect gene production and signaling expression. Cells in the serum-free medium were infected with VSV, SeV, H1N1 or HSV for 1.5 hrs. Then the supernatant was removed and cells were returned to the medium containing 10% FBS for the indicated times.
VSV infection in vivo
Eight weeks old mice and VSV were prepared for the in vivo viral infection by intraperitoneal injection or intranasal injection as described. The expression of IFNβ, IL-6, TNFα and VSVG were detected in the organs via q-PCR. 3 days of VSV-infected mice were collected for immunohistochemistry staining. Survival observation continued until 15 days.
TCID50 assay
Viral titers were determined by the 50% tissue culture infectious dose (TCID50) and standard curves were presented as described [45]. Briefly, Otud1+/+ or Otud1-/- MEFs were infected with either VSV or HSV for 10 hr. Cultural supernatants containing the viruses were diluted with DMEM serially, and then were put on the monolayer of Vero cells in 96-well plates with 8 repetitions. TCID50 was measured for viral titers after 3 days.
Immunohistochemistry assay
Lungs, livers and kidneys dissected from 3 day-infected mice were fixed in 4% formaldehyde solution and embedded into paraffin. Paraffin sections were placed on a slide and incubated with a VSVG antibody. The slides were then stained with hematoxylin-eosin solution. The histological changes were finally examined by the positive fluorescence microscopy.
Immunofluorescence microscopy
Immunofluorescence microscopy was performed as described previously [35]. Briefly, cells were infected with SeV for 4 hr or 8 hr, and then incubated with MitoTracker Red CMXRos (40741ES50, Yeasen) for 30 min. Cells were permeabilized with 0.5% Triton X-100 and blocked with 5% BSA, and then were incubated with either an anti-OTUD1 antibody (Abcam, ab122481) or an anti-Smurf1 (Santa Cruz, sc-100616) antibody overnight, followed by staining with either 488 goat anti-mouse IgG (Alexa Fluor, A11001) or 594 goat anti-rabbit IgG (Alexa Fluor, A11012). Cell nuclei were stained with DAPI for 30 min, and the fluorescent images were captured with the Nikon A1 confocal microscope.
Analysis of serum ELISA
IFNβ ELISA Kits (Elabscience, E-EL-M0033C) were used to test the concentrations of IFNβ protein in sera from VSV-infected mice for 24 hr.
Tissue RNA extraction
Tissues were extracted by the homogenizer from the infected mice. After the red blood cells were broken, tissue cells were cleaved with the Trizol reagent. Total RNAs were extracted according to the manufacturer's instructions (Invitrogen).
Cell culture, transfection and reagents
HEK293T, HeLa, HT1080, 2fTGH and HepG2 cells were obtained from ATCC. U3A cells were provided by Dr. Guoqiang Chen (Shanghai Jiao Tong University). Vero cells were gifts from Dr. Chunfu Zheng (Soochow University). All cells were cultured at 37°C under 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM; HyClone) supplemented with 10% FBS (GIBCO, Life Technologies), 100 units/ml penicillin, and 100 μg/ml streptomycin.
For transfection, LongTrans (UCallM, TF/07), PEI (Polyetherimide) and Lipofectamine 3000 (Invitrogen, L3000-015) were used. The plasmids and reagents required in the experiment were as following: Flag-OTUD1 plasmids were obtained from Dr. J Wade Harper (Harvard Medical School, Addgene plasmids). IFNβ (P125)-Luc, ISRE-Luc, Renilla, HA-Ub and mutant plasmids (HA-R48K, HA-R63K) were gifts from Dr. Serge Y. Fuchs (University of Pennsylvania). Flag-Smurf1, Flag-TRAF3, Flag-TRAF6 and Flag-MAVS were provided by Dr. Chengjiang Gao (Shandong University, China). Flag-OTUD1 (CH) was generated by QuickChange site-Directed Mutagenesis Kit (Stratagene). The reagents are as following: MG132 (Sigma, C2211), cGAMP (InvivoGen, tlrl-nacga23), Poly(I:C) (HMW) Rhodamine (InvivoGen, tlrl-picr), LPS (Sigma, L2630). ISD was a gift from Dr. Chunfu Zheng (Soochow University, China).
Q-PCR assay
Isolation of mRNAs from cells was performed by Trizol, and then reverse transcription kits (Thermo, K1622) were used for cDNA synthesis. The change-in-cycling-threshold (2-ΔΔCt) method was utilized for calculation of the relative gene expression levels. Quantification of all target genes was normalized to the control gene β-actin, and all data are shown as fold change normalized to that in either unstimulated or uninfected cells accordingly. The primers used in qPCR were listed: Otud1 forward, 5'-GGGGAGTTTATCATCGCTGCT-3' and reverse, 5'-TGAGCCAACTGAGCCAAATAC-3'; Smurf1 forward, 5'-ATTCGAT AACCATTAGCGTGTGG-3' and reverse, 5'-CGCCGGTTCCTATTCTGTCTC-3'; Traf3 forward, 5'-ATGCTGAGTGTGCACGACAT-3' and reverse, 5'-TAGAC CCTGGCACATCTTA-3'; Traf6 forward, 5'-GCACAAGATGGAACTGAGACA-3' and reverse, 5'-TGACATTTGCCAAAGGACAG-3'; Mavs forward, 5'-TAAAC AGGGTGCAGAGAGTGA-3' and reverse, 5'-GATTGGTGAGCGCATTAGAA-3'; SeV forward, 5'-GATGACGATGCCGCAGCAGTAG-3' and reverse, 5'-CCTC CGATGTCAGTTGGTTCACTC-3'; Vsvg forward,5'-ACGGCGTACTTCCAGAT GG-3' and reverse, 5'-CTCGGTTC AAGATCCAGGT-3'; H1N1 forward, 5'-TTC TAACCGAGGTCGAAACG-3' and reverse, 5'-ACAAAGCGTCTACGCTG CAG-3'; Ifit1 forward,5'-GCCTATCGCCAAGATTTAGATGA-3' and reverse, 5'-TTCTGGATTTAACCGGACAGC-3'; Isg15 forward,5'-GGTGTCCGTGACT AACTCCAT-3'and reverse, 5'-CTGTACCACTAGCATCACTGTG-3'; Isg20 forward, 5'-GAAGCGAAGGTTCTTGGAACA-3' and reverse, 5'-GCCATCTAC TCTTGAAGTTTCCC-3'; Isg54 forward,5'-GGAGAGCAATCTGCGACAG-3' and reverse, 5'-GCTGCCTCATTTAGACCTCTG-3'; Human-IFNβ forward, 5'-CATT ACCTGAAGGCCAAGGA-3' and reverse, 5'-CAGCATCTGCTGGTTGAAGA- 3'; Mouse-IFNβ forward, 5'-CTTCGTGTTTGGTAGTGATGGT-3' and reverse, 5'-GGGGATGATTTCCAGCCGA-3'; Il-6 forward, 5'-GGCGGATCGGATGTT GTGAT-3' and reverse, 5'-GGACCCCAGACAATCGGTTG-3' Actb forward, 5'-ACCAACTGGGACGACATGGAGAAA-3' and reverse, 5'-ATAGCACAGCCT GGATAGCAACG-3'.
Assay of luciferase activity
Cells were transfected with specific plasmids together with ISRE-Luc and Renilla plasmids. Cells were infected with viruses before harvested. P125-Luc plasmid was used in the IFNβ promoter assay. The luciferase activity was tested by using the Dual Luciferase Reporter Assay System (Promega, E1910). Three independent experiments were performed and were shown as the average mean ± standard derivation (s.d.).
Immunoprecipitation and immunoblotting
Immunoprecipitation and immunoblotting were carried out as described previously [46]. The following antibodies were used: IRF3 (1:1000, Santa Cruz, sc-9082), p-IRF3 (1:1000, Cell Signaling, 4947S), OTUD1 (1:1000, Abcam, ab182511), Flag (1:5000, Sigma, F7425), β-actin (1:5000, Proteintech, 66009-1-Ig), Myc (1:5000, Abmart, m2002), MAVS (1:1000, Santa Cruz, sc-166583), TRAF3 (1:1000, Cell Signaling, 4729S), TRAF6 (1:1000, Abcam, ab94720), TBK1 (1:1000, Cell Signaling, 3013S), RIG-I (1:1000, Cell Signaling, 4200S), Smurf1 (1:1000, Santa Cruz, sc-100616), Smurf2 (1:1000, Santa Cruz, sc-25511), Ub (1:1000, Santa Cruz, sc-8017), HA (1:5000, Abcam, ab9110), K48-Ub (1:1000, Cell Signaling, 4289S), and VSVG (1:5,000, Santa Cruz, sc-66180).
Statistical analysis
Statistical significance between groups was performed using two-tailed Student's t-test or Gehan-Breslow-Wilcoxon test. Densitometry quantification was made with ImageJ software. P values less than 0.05 were considered significant. *p < 0.05, **p < 0.01, ***p < 0.001; NS, not significant. Kaplan-Meier survival curves were generated and analyzed for mouse survival study performed in Graph Pad Prism 5.0.
Supporting information
Acknowledgments
We thank Eugene Y. Chinn for helpful comments about this paper. We thank Guoqiang Chen for U3A cells, Chengjiang Gao for Flag-MAVS, Flag-TRAF3 and Flag-TRAF6 constructs, Chen Wan for VSV and SeV, Chunfu Zheng for HSV and Vero Cells, and Jianfeng Dai for H1N1.
Data Availability
All relevant data are within the paper and its Supporting Information files.
Funding Statement
This study was supported by the National Natural Science Foundation of China (31370873 and 31570865), the Program of 1000 Young Talents, Jiangsu Provincial Distinguished Young Scholars (BK20130004), the program for Changjiang scholars and Innovative Research Team in University of Ministry of Education of China (PCSIRT-IRT1075) and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD). HZ is the recipient of all above funding. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
References
- 1.Barbalat R, Ewald SE, Mouchess ML, Barton GM. (2011) Nucleic acid recognition by the innate immune system. Annu Rev Immunol 29: 185–214. doi: 10.1146/annurev-immunol-031210-101340 [DOI] [PubMed] [Google Scholar]
- 2.Paludan SR, Bowie AG. (2013) Immune sensing of DNA. Immunity 38: 870–880. doi: 10.1016/j.immuni.2013.05.004 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 3.Rehwinkel J, Reis e Sousa C. (2010) RIGorous detection: exposing virus through RNA sensing. Science 327: 284–286. doi: 10.1126/science.1185068 [DOI] [PubMed] [Google Scholar]
- 4.Sun L, Wu J, Du F, Chen X, Chen ZJ. (2013) Cyclic GMP-AMP synthase is a cytosolic DNA sensor that activates the type I interferon pathway. Science 339: 786–791. doi: 10.1126/science.1232458 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 5.Yoneyama M, Kikuchi M, Natsukawa T, Shinobu N, Imaizumi T, et al. (2004) The RNA helicase RIG-I has an essential function in double-stranded RNA-induced innate antiviral responses. Nat Immunol 5: 730–737. doi: 10.1038/ni1087 [DOI] [PubMed] [Google Scholar]
- 6.Kawai T, Akira S. (2010) The role of pattern-recognition receptors in innate immunity: update on Toll-like receptors. Nat Immunol 11: 373–384. doi: 10.1038/ni.1863 [DOI] [PubMed] [Google Scholar]
- 7.Kawai T, Takahashi K, Sato S, Coban C, Kumar H, et al. (2005) IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction. Nat. Immunol 6: 981–988. doi: 10.1038/ni1243 [DOI] [PubMed] [Google Scholar]
- 8.Seth RB, Sun L, Ea CK, Chen ZJ. (2005) Identification and characterization of MAVS, a mitochondrial antiviral signaling protein that activates NF-kappaB and IRF 3. Cell 122: 669–682. doi: 10.1016/j.cell.2005.08.012 [DOI] [PubMed] [Google Scholar]
- 9.Belgnaoui SM, Paz S, Hiscott J. (2011) Orchestrating the interferon antiviral response through the mitochondrial antiviral signaling (MAVS) adapter. Curr Opin Immunol 23: 564–572. doi: 10.1016/j.coi.2011.08.001 [DOI] [PubMed] [Google Scholar]
- 10.Dixit E, Boulant S, Zhang Y, Lee AS, Odendall C, et al. (2010) Peroxisomes are signaling platforms for antiviral innate immunity. Cell 141: 668–681. doi: 10.1016/j.cell.2010.04.018 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 11.Liu S, Chen ZJ. (2011) Expanding role of ubiquitination in NF-kappaB signaling. Cell Res 21: 6–21. doi: 10.1038/cr.2010.170 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 12.Sadler AJ, Williams BR. (2008) Interferon-inducible antiviral effectors. Nat Rev Immunol 8: 559–568. doi: 10.1038/nri2314 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 13.Scott I. (2010) The role of mitochondria in the mammalian antiviral defense system. Mitochondrion 10: 316–320. doi: 10.1016/j.mito.2010.02.005 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 14.Sen GC, Sarkar SN. (2007) The interferon-stimulated genes: targets of direct signaling by interferons, double-stranded RNA, and viruses. Curr Top Microbiol Immunol 316: 233–250. [DOI] [PubMed] [Google Scholar]
- 15.Meylan E, Curran J, Hofmann K, Moradpour D, Binder M, et al. (2005) Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus. Nature 437: 1167–1172. doi: 10.1038/nature04193 [DOI] [PubMed] [Google Scholar]
- 16.Xu LG, Wang YY, Han KJ, Li LY, Zhai Z, et al. (2005) VISA is an adapter protein required for virus-triggered IFN-beta signaling. Mol Cell 19: 727–740. doi: 10.1016/j.molcel.2005.08.014 [DOI] [PubMed] [Google Scholar]
- 17.Hou F, Sun L, Zheng H, Skaug B, Jiang QX, et al. (2011) MAVS forms functional prion-like aggregates to activate and propagate antiviral innate immune response. Cell 146: 448–461. doi: 10.1016/j.cell.2011.06.041 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 18.Liu S, Chen J, Cai X, Wu J, Chen X, et al. (2013) MAVS recruits multiple ubiquitin E3 ligases to activate antiviral signaling cascades. ELife 2: e00785 doi: 10.7554/eLife.00785 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 19.Hacker H, Tseng PH, Karin M (2011) Expanding TRAF function: TRAF3 as a tri-faced immune regulator. Nat Rev Immunol 11: 457–468. doi: 10.1038/nri2998 [DOI] [PubMed] [Google Scholar]
- 20.Paz S, Vilasco M, Werden SJ, Arguello M, Joseph-Pillai D, et al. (2011) A functional C-terminal TRAF3-binding site in MAVS participates in positive and negative regulation of the IFN antiviral response. Cell Res 21: 895–910. doi: 10.1038/cr.2011.2 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 21.Saha SK, Pietras EM, He JQ, Kang JR, Liu SY, et al. (2006) Regulation of antiviral responses by a direct and specific interaction between TRAF3 and Cardif. EMBO J 25: 3257–3263. doi: 10.1038/sj.emboj.7601220 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 22.Hacker H, Redecke V, Blagoev B, Kratchmarova I, Hsu LC, et al. (2006) Specificity in Toll-like receptor signalling through distinct effector functions of TRAF3 and TRAF6. Nature 439: 204–207. doi: 10.1038/nature04369 [DOI] [PubMed] [Google Scholar]
- 23.Oganesyan G, Saha SK, Guo B, He JQ, Shahangian A, et al. (2006) Critical role of TRAF3 in the Toll-like receptor-dependent and -independent antiviral response. Nature 439: 208–211. doi: 10.1038/nature04374 [DOI] [PubMed] [Google Scholar]
- 24.Zeng W, Xu M, Liu S, Sun L, Chen ZJ. (2009) Key role of Ubc5 and lysine-63 polyubiquitination in viral activation of IRF3. Mol Cell 36: 315–325. doi: 10.1016/j.molcel.2009.09.037 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 25.You F, Sun H, Zhou X, Sun W, Liang S, et al. (2009) PCBP2 mediates degradation of the adaptor MAVS via the HECT ubiquitin ligase AIP4. Nat Immunol 10: 1300–1308. doi: 10.1038/ni.1815 [DOI] [PubMed] [Google Scholar]
- 26.Zhou X, You F, Chen H, Jiang Z. (2012) Poly(C)-binding protein 1 (PCBP1) mediates housekeeping degradation of mitochondrial antiviral signaling (MAVS). Cell Res 22: 717–727. doi: 10.1038/cr.2011.184 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 27.Wei C, Ni C, Song T, Liu Y, Yang X, et al. (2010) The hepatitis B virus X protein disrupts innate immunity by downregulating mitochondrial antiviral signaling protein. J Immunol 185: 1158–1168. doi: 10.4049/jimmunol.0903874 [DOI] [PubMed] [Google Scholar]
- 28.Mao AP, Li S, Zhong B, Li Y, Yan J, et al. (2010) Virus-triggered ubiquitination of TRAF3/6 by cIAP1/2 is essential for induction of interferon-beta (IFN-beta) and cellular antiviral response. J Biol Chem 285: 9470–9476. doi: 10.1074/jbc.M109.071043 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 29.Li S, Zheng H, Mao AP, Zhong B, Li Y, et al. (2010) Regulation of virus-triggered signaling by OTUB1- and OTUB2-mediated deubiquitination of TRAF3 and TRAF6. J Biol Chem 285: 4291–4297. doi: 10.1074/jbc.M109.074971 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 30.Peng Y, Xu R, Zheng X. (2014) HSCARG negatively regulates the cellular antiviral RIG-I like receptor signaling pathway by inhibiting TRAF3 ubiquitination via recruiting OTUB1. PLoS Pathog 10: e1004041 doi: 10.1371/journal.ppat.1004041 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 31.Karim R, Tummers B, Meyers C, Biryukov JL, Alam S, et al. (2013) Human papillomavirus (HPV) upregulates the cellular deubiquitinase UCHL1 to suppress the keratinocyte's innate immune response. PLoS Pathog 9: e1003384 doi: 10.1371/journal.ppat.1003384 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 32.Panda S, Nilsson JA, Gekara NO. (2015) Deubiquitinase MYSM1 Regulates Innate Immunity through Inactivation of TRAF3 and TRAF6 Complexes. Immunity 43: 647–659. doi: 10.1016/j.immuni.2015.09.010 [DOI] [PubMed] [Google Scholar]
- 33.Kayagaki N, Phung Q, Chan S, Chaudhari R, Quan C, et al. (2007) DUBA: a deubiquitinase that regulates type I interferon production. Science 318: 1628–1632. doi: 10.1126/science.1145918 [DOI] [PubMed] [Google Scholar]
- 34.Lin D, Zhang M, Zhang MX, Ren Y, Jin J, et al. (2015) Induction of USP25 by viral infection promotes innate antiviral responses by mediating the stabilization of TRAF3 and TRAF6. Proc Natl Acad Sci U S A 112: 11324–11329. doi: 10.1073/pnas.1509968112 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 35.Ren Y, Zhao P, Liu J, Yuan Y, Cheng Q, et al. (2016) Deubiquitinase USP2a Sustains Interferons Antiviral Activity by Restricting Ubiquitination of Activated STAT1 in the Nucleus. PLoS Pathog 12: e1005764 doi: 10.1371/journal.ppat.1005764 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 36.Sowa ME, Bennett EJ, Gygi SP, Harper JW (2009) Defining the human deubiquitinating enzyme interaction landscape. Cell 138: 389–403. doi: 10.1016/j.cell.2009.04.042 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 37.Wang Y, Tong X, Ye X. (2012) Ndfip1 negatively regulates RIG-I-dependent immune signaling by enhancing E3 ligase Smurf1-mediated MAVS degradation. J Immunol 189: 5304–5313. doi: 10.4049/jimmunol.1201445 [DOI] [PubMed] [Google Scholar]
- 38.Pan Y, Li R, Meng JL, Mao HT, Zhang Y, et al. (2014) Smurf2 negatively modulates RIG-I-dependent antiviral response by targeting VISA/MAVS for ubiquitination and degradation. J Immunol 192: 4758–4764. doi: 10.4049/jimmunol.1302632 [DOI] [PubMed] [Google Scholar]
- 39.Li S, Lu K, Wang J, An L, Yang G, et al. (2010) Ubiquitin ligase Smurf1 targets TRAF family proteins for ubiquitination and degradation. Mol Cell Biochem 338: 11–17. doi: 10.1007/s11010-009-0315-y [DOI] [PubMed] [Google Scholar]
- 40.Mevissen TE, Hospenthal MK, Geurink PP, Elliott PR, Akutsu M, et al. (2013) OTU deubiquitinases reveal mechanisms of linkage specificity and enable ubiquitin chain restriction analysis. Cell 154: 169–184. doi: 10.1016/j.cell.2013.05.046 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 41.Liu W, Li J, Zheng W, Shang Y, Zhao Z, et al. (2017) Cyclophilin A-regulated ubiquitination is critical for RIG-I-mediated antiviral immune responses. ELife 6: 24425. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 42.Xia P, Wang S, Xiong Z, Ye B, Huang LY, et al. (2015) IRTKS negatively regulates antiviral immunity through PCBP2 sumoylation-mediated MAVS degradation. Nat Commun 6: 8132 doi: 10.1038/ncomms9132 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 43.Zhou Y, He C, Yan D, Liu F, Liu H, et al. (2016) The kinase CK1varepsilon controls the antiviral immune response by phosphorylating the signaling adaptor TRAF3. Nat Immunol 17: 397–405. doi: 10.1038/ni.3395 [DOI] [PubMed] [Google Scholar]
- 44.Quicke KM, Diamond MS, Suthar MS. (2017) Negative regulators of the RIG-I-like receptor signaling pathway. Eur J Immunol 47: 615–628. doi: 10.1002/eji.201646484 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 45.Wang P, Xu J, Wang Y, Cao X. (2017) An interferon-independent lncRNA promotes viral replication by modulating cellular metabolism. Science 358:1051–1055. doi: 10.1126/science.aao0409 [DOI] [PubMed] [Google Scholar]
- 46.Li L, Qian G, Zuo Y, Yuan Y, Cheng Q, et al. (2016) Ubiquitin-dependent Turnover of Adenosine Deaminase Acting on RNA 1 (ADAR1) Is Required for Efficient Antiviral Activity of Type I Interferon. J Biol Chem 291: 24974–24985. doi: 10.1074/jbc.M116.737098 [DOI] [PMC free article] [PubMed] [Google Scholar]
Associated Data
This section collects any data citations, data availability statements, or supplementary materials included in this article.
Supplementary Materials
Data Availability Statement
All relevant data are within the paper and its Supporting Information files.