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. 2018 Jun;24(6):865–879. doi: 10.1261/rna.063206.117

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© 2018 Hönig et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 4. — Reduced steady-state free miR-29a-3p levels are detected in the organs of antimiR-29a-3p-injected dams. The steady-state free miR-29a-3p levels were assessed by real-time RT-PCR in the brain (B), heart (H), kidney (K), liver (Li), and lungs (Lu) of dams at postpartum day (PP)1 and PP14, after intravenous injection of either a scrambled antimiR (100 mg kg⁻¹) or antimiR-29a-3p (100 mg kg⁻¹) at gestational day 17. Data are presented as mean ΔΔCT, where ΔΔCT reflects ΔCT(antimiR-29a-3p treatment)–ΔCT(scrambled antimiR treatment), the negative value reflecting reduced detectable free miR-29a-3p in antimiR-29a-3p-injected dams. The original ΔCT values were obtained by ΔCT = CT_(Rnu6) − CT_(miR-29a-3p).