Abstract
The fruit fly Drosophila melanogaster is a powerful model system for the study of innate immunity in vector insects as well as mammals. For vector insects, it is particularly important to understand all aspects of their antiviral immune defenses, which could eventually be harnessed to control the transmission of human pathogenic viruses. The immune responses controlling RNA viruses in insects have been extensively studied, but the response to DNA virus infections is poorly characterized. Here, we report that infection of Drosophila with the DNA virus Invertebrate iridescent Virus 6 (IIV-6) triggers JAK-STAT signaling and the robust expression of the Turandots, a gene family encoding small secreted proteins. To drive JAK-STAT signaling, IIV-6 infection more immediately induced expression of the unpaireds, a family of IL-6-related cytokine genes, via a pathway that required one of the three Drosophila p38 homologs, p38b. In fact, both Stat92E and p38b were required for the survival of IIV-6 infected flies. In addition, in vitro induction of the unpaireds required an NADPH-oxidase, and in vivo studies demonstrated Nox was required for induction of TotA. These results argue that ROS production, triggered by IIV-6 infection, leads to p38b activation and unpaired expression, and subsequent JAK-STAT signaling, which ultimately protects the fly from IIV-6 infection.
Author summary
Mosquitoes and other biting insects transmit many harmful pathogens to humans, including parasites and viruses. In order to better protect humans from these diseases, we must gain a more complete understanding of how insects successfully—or unsuccessfully—combat these infections. While we know a great deal regarding how insects combat RNA viruses, we know little about their immune response to DNA virus infections. Studies of DNA virus infections may reveal novel immune mechanisms, which could be uniquely effective against DNA virus infections or could be broadly effective against many viruses. In this study, we utilized an invertebrate DNA virus, IIV-6, infection model with the fruit fly Drosophila melanogaster, and found that virus infection activated several innate immune signaling pathways, which help protect the animal against this virus. Eventually, a more complete understanding of the antiviral responses of insects may be useful to restrict virus infections of disease transmitting insects.
Introduction
Like all multicellular organisms, insects face a constant threat of infection from a wide array of microorganisms, including viral, bacterial, and fungal pathogens. Insects combat these infections with both static and inducible defenses, including a chitinous exoskeleton, circulating phagocytes and the induction of host defense genes, such as antimicrobial peptides [1]. For example, Drosophila melanogaster respond to bacterial and fungal infections through two conserved NF-κB signaling pathways, Toll and Imd, that drive the production of antimicrobial peptides and other inducible host defense molecules. Toll and Imd pathways are homologous to the TLR-MyD88 and TLR-TRIF signaling pathways in mammals, respectively. Unlike mammals, the Drosophila NF-κB pathways seem to play a limited role in response to invertebrate viral infections [2–5].
Insects, especially mosquitoes, are major vectors of arboviral diseases, and characterizing the pathways that they utilize to combat viral infections is necessary to gain a complete understanding of disease transmission. Drosophila, also a dipteran insect, has served as a productive model for studying insect antiviral immunity [6]. In Drosophila, the most intensely studied antiviral pathway is the siRNA response. The siRNA response is triggered when viral dsRNA intermediates, either derived directly from the viral genome or produced as an intermediate during replication or transcription, are recognized by Dicer-2, which processes these dsRNAs into 21 base-pair fragments and loads them onto Argonaute-2. This complex, termed an RNA-Induced Silencing Complex (RISC), is then able to destroy its complementary target sequence. This mechanism is a potent antiviral defense against RNA viruses and, in some circumstances, against DNA viruses [7, 8]. In addition, it has been suggested that Dicer-2, following the recognition of viral dsRNA, can trigger a signaling pathway that induces the transcription of the antiviral gene Vago [9]. While antiviral effects of Vago have only been shown upon Drosophila C virus (DCV) infection of flies, mosquito Vago was induced upon flavivirus infections of Culicine cell lines and animals and was suggested to be antiviral [10, 11].
On the other hand, mammalian antiviral defenses are triggered after recognition of an array of pathogen-associated and damage-associated molecular patterns (PAMPs and DAMPs), including but not limited to dsRNA. For example, various RNA species are recognized in the endosome by TLR3, 7 & 8, or in the cytosol by RIG-I or MDA-5. In addition, numerous viral proteins are recognized directly by various TLRs [12], and viral-induced damage often leads to induction of inflammasome activation and pyroptosis or necroptosis. Inflammasome activation results in the production of proinflammatory cytokines to promote recruitment of effector cells to the site of infection, while pyroptosis can act to restrict viral infection by killing the infected cell and thereby limiting viral replication [13].
Compared to mammalian systems, the extent to which the invertebrate immune system recognizes PAMPs and DAMPs, beyond viral dsRNA, is less well-studied. Examples from Drosophila include Toll-7, which was demonstrated to sense vesicular stomatitis virus (VSV) and Rift Valley fever virus (RVFV), and activate autophagy as an antiviral defense [14, 15]. Additionally, JAK-STAT signaling has been shown to induce vir-1 in response to DCV or cricket paralysis virus (CrPV), although the mechanism by which these viruses activate JAK-STAT signaling is unknown [8]. Imd signaling in the gut, triggered by commensals, is required for the activation of the ERK pathway, which protects against several enteric RNA viruses [5]. This commensal-mediated priming may explain the previously reported activity of Imd and Toll pathways in protecting against other viral infections [2–4, 16]. A study examining the origins of cGAS-STING signaling—an important cytosolic DNA sensing pathway in mammals, leading to Type I interferon production—concluded that cGAS homologs in insects lack regions required for DNA binding [17]. Thus, the role of the cGAS homologs in insects remains unclear.
In fact, the pathways responding to DNA virus infections in Drosophila have not been characterized. Insect DNA viruses, such as baculoviruses, the invertebrate iridescent viruses, and polydnaviruses are large, complex viruses ranging from 50 to more than 200 genes. These viral genes include many immunomodulators and other proteins that manipulate the cellular environment to facilitate replication [18]. These large DNA viruses and their myriad of host targeting and manipulating factors suggest that many host immune response pathways, beyond RNAi, interfere with viral replication.
Here, we report that Drosophila infection with the DNA virus Invertebrate iridescent virus 6 (IIV-6) induces a p38b-dependent pathway that activates JAK-STAT signaling and the robust transcriptional induction of a class of small secreted proteins known as the Turandots. Activation of this pathway requires the production of reactive oxygen species, perhaps indicative of a response to damage. Moreover, two key components of this pathway, p38b and Stat92E, are essential for host defense against IIV-6 infection.
Results
Turandots are induced by IIV-6 infection
Given the paucity of data on the insect response to DNA virus infection, we sought to identify the immune-related genes upregulated upon IIV-6 infection of adult flies. Male flies were injected with PBS (vehicle control) or IIV-6 at 1x104 TCID50 for 12, 24, or 48 hours, RNA was isolated and analyzed by NanoString nCounter Analysis, with a codeset probing 139 immune-related genes (Fig 1A). Two members of the Turandot (Tot) family, TotA and TotM were strongly upregulated at all time points in the IIV-6 infected samples compared to PBS-injected controls (Fig 1A and 1A’). These genes are part of a family of eight closely related, rapidly evolving genes that are induced by a variety of stressors including bacterial infection, heat shock, mechanical pressure, and UV-exposure [19, 20]. The Tots encode for small secreted proteins that have no known function [19, 21]. As the NanoString codeset included only two Tot genes, we used qRT-PCR to examine the expression of all eight Tot genes following IIV-6 infection. Six Tot genes were induced 10–1000 fold, compared to the PBS injected controls, 6 to 24 hours following IIV-6 infection (Fig 1B). The two Tot genes not up-regulated, TotE and TotF, are clustered together on Chromosome 2, suggesting that these two closely related Tots may respond to a different set of stimuli. Note, TotE was undetectable, while TotF was detected but unchanged by IIV-6 infection. Drosophila S2* cells also induced TotA, peaking between 24–36 hours after IIV-6 infection, while in mock-treated controls TotA expression remained at baseline levels (Fig 1C).
To begin to dissect the mechanisms that lead from DNA virus infection to Tot gene induction, we tested the requirement for live virus infection and viral replication in this process. IIV-6 was heat- or UV-inactivated, which typically creates virus that can attach, enter cells, and possibly deliver damaged nucleic acids or protein but is not replicative [22]. These inactivated viruses were then used to stimulate S2* cells (Fig 2A). Both UV-inactivation and heat treatment significantly reduced TotA induction, to levels near baseline. In addition, infection with another DNA virus, Vaccinia virus (VACV), which is known to abortively infect S2 cells [23, 24], failed to induce TotA expression (Fig 2A). Consistent with this observation, pretreatment of S2* cells with viral DNA polymerase inhibitors, phosphonoacetic acid (PAA) or cidofovir, also resulted in significantly diminished IIV-6 triggered TotA induction (Fig 2B and 2C). These results suggest that the presence of viral DNA alone is not sufficient to trigger Tot induction, in contrast to mammalian systems where cytosolic DNA triggers a robust cytokine response and triggers inflammasome activation [25]. Studies with the polymerase inhibitors PAA and cidofovir further argue that virus entry and expression of early gene transcripts are also not sufficient for this response. Together, these data indicate that active viral replication (or the processes downstream of viral replication) is required for IIV-6 induced TotA induction.
Turandot induction is JAK-STAT dependent
Previous studies demonstrated that the JAK-STAT signaling pathway is responsible for inducing Tot expression after Gram-negative bacterial infection [26]. To test whether JAK-STAT signaling is also required for IIV-6-induced Tot expression, domeless, hopscotch, and Stat92E, the sole Drosophila homologs for the gp130 receptor, JAK, and STAT, respectively, were targeted by RNAi in S2* cells. Knockdown of any of these genes led to a significant decrease in TotA induction (Fig 3A, knock-down efficiencies S1A–S1C Fig). We then asked whether the JAK-STAT pathway had any effect on survival following IIV-6 infection. Stat92E was ubiquitously knocked down using the tubulin-GAL4 driver and these flies were challenged with 104 TCID50 of IIV-6. The Stat92E knockdown flies exhibited significantly increased lethality compared to the control strain (progeny of w1118 x tubulin-GAL4) after virus infection (Fig 3B, additional trials S2 Fig, statistical analysis for all survival assays can be found in S2 Table). On the other hand, both control and Stat92E knockdown lines tolerated the control PBS injection to a similar degree. This was confirmed using additional RNAi lines (Figs 3C and S1E), and all lines had significant knock-down efficiencies (S1D Fig). These results demonstrate that IIV-6 induced Tot expression is controlled by the JAK-STAT pathway, and this pathway is critical for survival following infection.
Next, we hypothesized that IIV-6 infection may induce the expression of one or more of the unpaireds, which encode the ligands for the gp130-like receptor Domeless. Unpaired 1, 2, and 3 are all distantly related to IL-6 [27]. Unpaired 1 is critical for embryonic development, and upd1 null flies are embryonic lethal, while the upd2, upd3 double deletion is viable. Unpaired 3 is induced in hemocytes after septic injury [26] and plays a role in gut regeneration following damage [28]. In S2* cells, IIV-6 infection induced expression of all three unpaireds, ~104-fold as measured by qRT-PCR (Fig 4A). Hypomorphic alleles of upd1, also known as outstretched, are viable and some of these alleles, such as oss, also affect the expression of upd3, which lies nearby [29]. In adult flies, IIV-6 induced TotA expression was significantly reduced in all upd alleles, with the largest decrease in the oss allele (Fig 4B). TotM induction was similarly reduced by oss to levels observed in the PBS injected control, with lesser but still significant reductions in the upd3 mutant and upd2Δ,upd3Δ double mutant. Given the phenotypes in the hypomorphic oss allele and the upd2Δupd3Δ double mutant, these data indicate that the unpaireds function redundantly to drive JAK-STAT signaling in response to IIV-6 infection.
The IIV-6 triggered expression of upds suggests that virus infection may induce these cytokine genes, which in turn will drive JAK-STAT signaling and Tot expression. In other systems, such as gut renewal, it has been suggested that various MAPK pathways are responsible for driving upd expression [28, 30–32]. Therefore S2* cells were treated with inhibitors targeting the three Drosophila MAPKs (JNK, p38, or ERK) and then infected with IIV-6. Treatment with p38 inhibitor significantly reduced upd3 expression to near baseline levels, while ERK inhibitor had no effect and JNK inhibitor actually increased upd3 expression (Fig 4C). On the other hand, the JAK-STAT inhibitor Tofacitinib blocked IIV-6 induced TotA expression but had no effect on the expression of upd3 (Fig 4D). Together, these data indicate that while JAK-STAT is required for Tot induction, the virus-triggered expression of the upds (JAK-STAT activating cytokines) involves the p38 MAPK pathway.
In vivo, p38b is required for IIV-6 induced TotA expression and survival from infection
Drosophila encode three p38 homologs, p38a, p38b, and p38c, with p38a and p38c juxtaposed on third chromosome and p38b on the second. p38a/c mutant flies are susceptible to oxidative stress and heat shock, though not to osmotic shock [33], while p38b has been shown to provide protection against pathogenic bacterial or fungal challenge, although the underlying mechanisms are unclear [34]. p38c has also been implicated in gut homeostasis and reactive oxygen species (ROS) production in the gut upon infection with P. entomophila or Erwinia carotovora carotovora 15 [35]. In addition, p38c is required for the induction of DOPA decarboxylase, which is required for the production of antimicrobial quinones produced in response to wounding [36]. p38b has also been linked to tolerance to infection with Salmonella typhimurium [37]. Mutant flies for all three p38 homologs are viable to adulthood and were infected with IIV-6 to determine their ability to induce Tots. We found that p38a and p38c null flies displayed normal levels of Tot expression following IIV-6 infection, while p38b null flies had only basal levels of Tot expression (Fig 5A). Furthermore, p38b heterozygous flies also expressed wild-type levels of Tots upon IIV-6 infection. Consistent with these TotA expression data, p38b mutants but not p38a mutants were hypersusceptible to IIV-6 infection (Fig 5B and 5C, with additional trials in S4 and S5 Figs). Likewise, p38b RNAi, when expressed with a ubiquitous driver (tubulin-GAL4), exhibited significantly reduced survival following IIV-6 infection compared to wild-type (driver alone) controls (Fig 5D, additional trials S6 Fig), while p38aRNAi lines survived similarly to controls (Fig 5E, additional trials, S7 Fig). The knock-down efficiencies for these lines are shown in S3 Fig These combined results show that p38b is required for Tot induction and survival following IIV-6 infection in adult flies. The S2* cell data further demonstrates that p38 is required for unpaired induction. Together, these data suggest that some aspect of viral infection triggers p38b activation leading to Unpaired production, JAK-STAT activation, and Tot induction.
These results prompted us to ask whether these survival defects indicate a direct antiviral role for p38b, or whether p38b promotes tolerance to IIV-6. To discern between these possibilities, viral genomes were quantified by QPCR from p38b heterozygous and homozygous strains. p38b homozygous mutant flies showed no increase in viral titer compared to heterozygous p38b siblings, suggesting that p38b promotes tolerance (Fig 5F).
Viral infection damages cells, often inducing cell death (both apoptotic and necrotic) as well as the acute production of antivirals. These responses to viral infections can also trigger the release of endogenous activators of inflammation, so called DAMPs. These DAMPs often lead to MAPK activation. In particular, it has been shown that ROS can trigger p38 MAPK signaling [38][31]. Therefore, we sought to determine whether ROS generation is required for IIV-6-triggered, p38-dependent unpaired expression. To this end, we treated S2* cells with diphenyleneiodonium chloride (DPI), an NADPH oxidase inhibitor, prior to infection with IIV-6. DPI treatment completely abrogated induction of both upd3 (Fig 6A) and TotA (Fig 6B) 24 hours after IIV-6 infection. To investigate which of the two NADPH oxidases encoded by Drosophila is responsible for this effect, we utilized previously characterized RNAi lines targeting each oxidase [39], with a fat-body specific expression (c564 driver). Nox knockdown, but not Duox, prevented IIV-6-triggered TotA induction (Fig 6C). It is noteworthy that Nox has previously been implicated to function in the sterile injury response, in particular the response to extracellular actin, within the fat body [38]. These results argue that IIV-6 infection triggers ROS production through Nox, that, in turn, stimulates p38b activation, Upd production and Tot induction.
Discussion
Here, we show that infection of Drosophila with the DNA virus IIV-6 triggers a protective p38b-dependent response (see pathway model in Fig 7). While previous work has demonstrated that Drosophila p38b is critical for survival to bacterial or fungal infections and affects the tolerance to bacterial infections [34, 37], this is the first time p38b has been linked to antiviral defenses. Critical targets for p38b for the protection against IIV-6 infection are the unpaireds, a family of three IL-6-like genes clustered together on Chromosome X. The genetic data presented here argue that the three Unpaireds function together, in a partially redundant manner, to activate the JAK-STAT pathway following IIV-6 infection, thereby driving Tot gene expression. The JAK-STAT pathway also protects against IIV-6 infection, although the role of the Tots in antiviral defense requires more study. These results also imply that p38b is activated following IIV-6 infection. While the mechanisms leading from virus infection to p38 activation are unclear, they likely involve ROS-mediated signaling as the induction of TotA expression is potently blocked by an NADPH oxidase inhibitor and require the Nox gene. This is reminiscent of the activation of p38a by ROS generated from apoptotic cells in models of tissue regeneration [31].
Interestingly, p38b has also been shown to provide tolerance to Salmonella typhimurium infections, promoting survival of the host without reducing bacterial burden [37]. This study suggested that p38b contributes to tolerance by enabling hemocyte enlargement, and hence, engulfment of larger quantities of bacteria. In the context of IIV-6 infection, p38b could be acting to promote engulfment of infected and damaged cells, thereby providing a repair mechanism to enable the animals to better tolerate and limit virus infection. Future studies will be necessary to probe all the roles of p38b in antiviral defense.
Although the data presented here demonstrate that the JAK-STAT pathway is protective against IIV-6 infection, the protective mechanisms require further study. In the case of the RNA virus DCV, the JAK-STAT pathway is also protective, possibly through the induction of vir-1. However, the JAK-STAT pathway is not broadly antiviral and vir-1 was not induced by IIV-6 [8]. Curiously, a previous study examining the role of the JAK-STAT pathway during IIV-6 infection, using one particular hypomorphic allelic combination hopscotch (JAK), concluded that hopscotch (and by inference the JAK-STAT pathway) was not involved in protecting flies against IIV-6 infection [7]. Our data, with multiple RNAi lines targeting stat92E, as well as the S2 cell based results with RNAi targeting domeless, hopscotch, and stat92E, demonstrate a consistent and reproducible role for this pathway in the response to and survival from IIV-6 infection. We believe these contradictory outcomes may be due to differences in alleles used or dose delivered.
The Tots are intriguing candidates for JAK-STAT induced antivirals. They are rapidly evolving with evidence of positive selection, typical for immune effectors [20, 40]. However, the Tots have not yet been demonstrated to provide direct antimicrobial activity. To date, we have been unable to demonstrate any antiviral activity for the Tots. In particular, over-expression of TotA resulted in reduced survival following IIV-6 infection and no change in viral titers (S8 Fig), consistent with the previously reported general toxicity caused by over expression of this gene [21]. Further studies, examining all six of the IIV-6 induced Tots, with both loss- and gain-of-function approaches, will be necessary to more fully examine this possibility.
The sensitivity of STAT knockdowns to IIV-6 infection argues that JAK-STAT signaling is an important antiviral target of p38b. However, other p38b targets are also possible. For example, an established target of p38b is the heat shock response. In the context of bacterial and fungal infections, p38b is known to regulate Heat shock factor (Hsf) expression and the induction of heat shock proteins (Hsps) [34]. In addition, another report has shown that Hsf protects flies against both RNA and DNA viral infections [41]. Together, these results suggest that the antiviral effects of p38b could be mediated, at least in part, through Hsf and Hsps. Indeed, Hsf mutant flies display an increased rate of death after IIV-6 infection. It will be interesting to learn if the heat shock response is activated by p38b following IIV-6 infection, and how this response interacts with JAK-STAT dependent viral protection.
Successful host defenses detect multiple characteristics of an invading pathogen. For example, cellular damage is one common indicator of pathogenic infection that can be sensed by the innate immune system. In mammals, several danger-associated molecular patterns (DAMPs) have been characterized, including HMGB1, F-actin, and histones [42–44]. Likewise, a recent report examining a Drosophila model of sterile injury demonstrated that extracellular actin activates JAK-STAT signaling [38]. In this paradigm, detection of extracellular actin, via an unknown receptor, triggered Nox-dependent ROS generation, the activation of Src42A and Shark (Syk homolog), and induction of unpaireds and eventually Tots. This pathway is very similar to that reported here, although p38b was not examined in this actin-DAMP, and suggests that IIV-6 infection may cause cellular damage, rupture and the release of actin, which in turn triggers ROS production, unpaired expression, JAK-STAT signaling and the induction of Tots. Formally testing this model will be facilitated by the identification of an extracellular actin receptor.
In summary, we have found a novel role for Drosophila p38b in protecting against DNA virus infection. Virus infection leads to p38b dependent responses, including the induction of the JAK-STAT activating cytokines, the Unpaireds, and the induction of downstream target genes such as the Tots. Based on the analysis of viral load, the p38b pathway appears to function primarily by increasing tolerance to IIV-6, as viral loads were not altered in the p38b strain. Whether the Tots contribute to this tolerance and, more generally, whether p38b induces a directly antiviral response, or relies entirely on the Unpaired and JAK-STAT signaling for its ability to tolerize against this viral infection will be probed in future studies.
Methods
Reagents
p38 inhibitor SB203580 (CAT#13067) [45], JNK inhibitor SP600125 (CAT#10010466) [46], JAK inhibitor Tofacitinib CP690,550(CAT#11598) [47], and ERK inhibitor U-0126(CAT#70970) [48–50], were purchased from Cayman Chemical. Diphenyleneiodonium chloride DPI (CAS#4673-26-1) [39] was purchased from Sigma-Aldrich. All inhibitors were dissolved in DMSO and used at the indicated concentrations.
Fly stocks and infections
p38aMPK1, p38bex9/CyO, p38c1A1/TM6, and p38c7B&/TM6 flies were a kind gift of Bruno Lemaitre. UAS-TotA flies were a kind gift of Dan Hultmark. NoxRNAi and DuoxRNAi lines were generated by Won-Jae Lee [39] and obtained from Andreas Bergmann. stat92ERNAi, w1118; P{UAS-STAT92EGD4492RNAi}v43866, and w1118; P{UAS-p38aGD17018RNAi}v52277 were obtained from the Vienna Drosophila Resource Center (VDRC). y1 v1; P{p38bTRiP.JF03341RNAi}attP2, Ab(1)oss, upd1os-s upd3os-s(BIN#79), Df(1)oso, upd1os-o upd3os-o(BIN#78),w* upd2Δ (BIN#55727), w* upd3Δ (BIN#55728),w* upd2Δupd3Δ: (BIN#55729), stat92ERNAi-2, y1 v1; P{TRiP.HMS00035} attP (BIN#33637), stat92ERNAi-1, y1 v1; P{TRiP.JF01265} attP2 (BIN#31317), were obtained from Bloomington Drosophila Stock Center (BDSC). w1118 flies were used as an immunologically wild-type control in all experiments, as these are the most similar background to the alleles listed above.
Three to five day old flies, maintained at 22°C, were used for all experiments. Flies were injected intrathoracically with 32.2 nL of virus (1x104 TCID50) or vehicle (PBS) using a Nanoject II (Drummond). For survival assays, a minimum of fifty flies were used per treatment, per genotype and the dead were counted daily. Kaplan-Meier curves are shown and significance was determined by log-rank (Mantel-Cox) using GraphPad Prism. For qRT-PCR analysis, at least three independent replicates of 15–20 flies each were used for RNA extraction. In all cases, three or more independent replicates, as indicated in each figure legend, were performed in parallel on the same day. At least 2 additional trials, each with 3 or more biologically independent replicates, were performed at other times, with similar results.
nCounter analysis
The expression levels of 139 Drosophila immune genes were assayed from 100 nanograms of RNA via a customized Nanostring nCounter codeset. Two biological replicates of 10–15 adult male flies 3–5 days of age were analyzed for each treatment and timepoint. The results were analyzed using nSolver 3.0 software according to the manufacturers instructions (NanoString Technologies, Seattle, WA, USA), and the heatmap was created using nSolver 3.0 software and JavaTree.
RNA isolation and qRT-PCR
Total RNA from flies or S2* cells was extracted using TRIzol (Invitrogen). Samples were then DNase treated (RQ1, Promega) and RNA re-extracted by phenol-chloroform. cDNA was synthesized using iScript cDNA Synthesis kit (BioRad). Alternatively, the gDNAclear cDNA synthesis kit (BioRad) was used following TRIzol purification. qRT-PCR was analyzed normalizing to the housekeeping gene Rp49. Primer sequences can be found as S1 Table. Cycling conditions: 50°C, 2 minutes; 94°C, 2 minutes; 95°C, 15 seconds; 61°C, 30 seconds; 72°C, 30 seconds; plate read, amplification cycle repeated 39 times. Melt curve performed and plate read, 58°C-95°C, 0.5°C increments. 10°C for 5 minutes. Cycling conditions for IIV-6 QCPR: 50°C, 2 minutes; 94°C, 2 minutes; 95°C, 15 seconds; 72°C, 1 minute; plate read, two-step amplification cycle repeated 39 times. Melt curve performed and plate read, 58°C-95°C, 0.5°C increments. 10°C for 5 minutes.
Cell culture and RNAi
dsRNA was produced as previously described, using primers and cycling conditions as recommended by the DRSC (Drosophila RNAi Screening Center) [51, 52]. Primer sequences can be found in S1 Table. S2* cells were cultured as previously described [53, 54] and were transfected with 3μg of dsRNA using Cellfectin II reagent (Invitrogen). Cells were split 24 hours after transfection to 5x105 cells/mL and 24 hours later cells were infected with IIV-6 at an MOI of 2. As a control, cells were mock-treated with the same volume of PBS (virus diluent) as used in infections. Cells were harvested in TRIzol (Invitrogen) 24 hours post-infection. In experiments with small molecule inhibitors, cells were treated with the indicated inhibitor at the stated concentration or the appropriate vehicle control 1 hour prior to virus infection.
Virus preparation
IIV-6 was provided by Luis Teixeira. IIV-6 was propagated and purified on DL-1 cells as previously described (9), with a final resuspension in PBS, and quantified on DL-1 cells by TCID50. Cells were infected at an MOI of 2 unless otherwise noted, while flies were injected with 1x104 TCID50, as detailed above.
Supporting information
Acknowledgments
We thank Luis Teixeria (Instituto Gulbenkian de Ciência, Oeiras, Portugal) for virus stocks, Bruno Lemaitre (École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland), Dan Hultmark (Umeå University, Umeå, Sweden), and the Bergmann lab (UMass Medical School, Worcester, United States) for fly stocks, Don Gammon (UT Southwestern, Dallas, United States) for virus aliquots and thoughtful discussions, and the Bloomington Stock Center at Indiana University and Vienna Drosophila Resource Center for fly stocks. We thank the Silverman and Pukkila-Worley labs, and Florentina Rus for advice and discussions.
Data Availability
All relevant data are within the paper and its Supporting Information files.
Funding Statement
This work was supported by grants to NS from the National Institute of Allergy and Infectious Diseases, R21 AI131695 and R01 060025. CW was supported by National Institute of Allergy and Infectious Diseases training grants T32 AI095213 and NIH T32 AI007349. The funders had no role in the study design, data collection and analysis, decision to publish or preparation of the manuscript.
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