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. 1990 Feb;81(2):153–160. doi: 10.1111/j.1349-7006.1990.tb02542.x

Production and Characterization of a Human Monoclonal Antibody Recognizing a New Antigen Expressed on Some Lymphoid Cells

Nobunao Ikewaki 1,✉, Hidekazu Tamauchi 2, Noboru Yamaura 1, Hiroomi Takahashi 3, Hidetoshi Inoko 4
PMCID: PMC5963900  PMID: 2139642

Abstract

A cell line secreting a human monoclonal antibody was established by Epstein‐Barr virus transforming B cells derived from an enlarged cervical lymph node excised from a patient bearing a carotid body tumor. The reactivity of the monoclonal antibody, designated as mNISP, was tested on various cells and cell lines. An antigen defined by the mNISP was expressed on some Burkitt's lymphoma cell lines and on a non‐T non‐B acute lymphoblastic leukemia cell line. Furthermore, this antigen was expressed on leukemic cells from 2 of 8 patients with chronic myelocytic leukemia, 2 of 10 patients with acute myeloblastic leukemia, one of 13 patients with acute lymphoblastic leukemia, and two patients with adult T cell leukemia, but it was not expressed on normal T, B and adherent (macrophage) cells. In addition, mNISP reacted with T cells obtained from human T‐cell leukemia virus type I carriers. We found that the antigen defined by mNISP was distinct from any previously reported antigen in terms of its pattern of cellular expression and molecular weight, suggesting that mNISP recognizes a new antigen expressed on some lymphoid cells.

Keywords: Human monoclonal antibody, Epstein‐Barr virus transformation, Lymphoid cells

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REFERENCES

  • 1. ) Davidge‐Pitts , K. J. and Pantanowitz , D.Carotid body tumors . Surg. Ann. , 16 , 203 – 225 ( 1984. ). [PubMed] [Google Scholar]
  • 2. ) Lack , E. E. , Cubilla , A. L. , Woodruff , J. M. and Farr , H. W.Paragangliomas of the head and neck region . Cancer , 39 , 397 – 409 ( 1977. ). [DOI] [PubMed] [Google Scholar]
  • 3. ) Saldanha , M. J. , Salem , L. E. and Travezan , R.High altitude hypoxia and chemodectoma . Hum. Pathol. , 4 , 251 – 263 ( 1973. ). [DOI] [PubMed] [Google Scholar]
  • 4. ) Grufferman , S. , Gillman , M. W. , Pasternak , L. R. , Peterson , C. L. and Young , W. G.Familial carotid body tumors . Cancer , 46 , 2116 – 2122 ( 1980. ). [DOI] [PubMed] [Google Scholar]
  • 5. ) Kroll , A. J.Hereditary deficiencies of clotting factors VII and × associated with carotid‐body tumors . N. Engl. J. Med. , 270 , 6 – 13 ( 1964. ). [DOI] [PubMed] [Google Scholar]
  • 6. ) Yagi , A. , Takahashi , H. , Yano , K. , Sano , H. and Nitta , K.The two cases of carotid body tumor in the same family . J. Otolaryngol. Jpn. , 91 , 1987 – 1995 ( 1988. ) ( in Japanese ). [DOI] [PubMed] [Google Scholar]
  • 7. ) Boyum , A.Separation of white blood cells . Nature , 204 , 793 – 794 ( 1964. ). [DOI] [PubMed] [Google Scholar]
  • 8. ) Lebien , T. W. , Hurwitz , R. L. and Kersey , J.Characterization of a xenoantiserum produced against 3M KCl‐solubilized antigen obtained from a non‐T non‐B (Pre‐B) acute lymphoblastic leukemia cell line . J. Immunol. , 122 , 82 – 88 ( 1979. ). [PubMed] [Google Scholar]
  • 9. ) Morimoto , C. , Todd , R. F. , Distaso , J. A. and Schlossman , S. F.The role of the macrophage in in vitro primary anti‐DNP antibody production in man . J. Immunol. , 127 , 1137 – 1141 ( 1981. ). [PubMed] [Google Scholar]
  • 10. ) Jondal , M. , Holm , G. and Wigzell , H.Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells . J. Exp. Med. , 136 , 207 – 215 ( 1972. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 11. ) Reinherz , E. L. , Kung , P. C. , Goldstein , G. and Schlossman , S. F.A monoclonal antibody with selective reactivity with functionally mature human thymocytes and all peripheral human T cells . J. Immunol. , 123 , 1312 – 1317 ( 1979. ). [PubMed] [Google Scholar]
  • 12. ) Ritz , J. , Pesando , J. M. , Notis‐McConarty , J. , Lazarus , H. and Schlossman , S. F.A monoclonal antibody to human acute lymphoblastic leukemia antigen . Nature , 283 , 583 – 585 ( 1980. ). [DOI] [PubMed] [Google Scholar]
  • 13. ) Griffin , J. D. , Ritz , J. , Nadler , L. M. and Schlossman , S. F.Expression of myeloid differentiation antigens on normal and malignant myeloid cells . J. Clin. Invest. , 68 , 932 – 941 ( 1981. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 14. ) Nadler , L. M. , Anderson , K. C. , Marti , G. , Bates , M. , Park , E. , Daley , J. F. and Schlossman , S. F.B4, a human B lymphocyte‐associated antigen expressed on normal, mitogen‐activated, and malignant B lymphocytes . J. Immunol. , 131 , 244 – 250 ( 1983. ). [PubMed] [Google Scholar]
  • 15. ) Stashenko , P. , Nadler , L. M. , Hardy , R. and Schlossman , S. F.Characterization of a human B lymphocyte‐specific antigen . J. Immunol. , 125 , 1678 – 1685 ( 1980. ). [PubMed] [Google Scholar]
  • 16. ) Fox , D. A. , Hussey , R. E. , Fitzgerald , K. A. , Acuto , O. , Poole , C. , Palley , L. , Daley , J. F. , Schlossman , S. F. and Reinherz , E. L.Tal, a novel 105 KD T cell activation antigen defined by a monoclonal antibody . J. Immunol. , 133 , 1250 – 1256 ( 1984. ). [PubMed] [Google Scholar]
  • 17. ) Lebien , T. W. , Boue , D. R. , Bradley , J. G. and Kersey , J. H.Antibody affinity may influence antigenic modulation of the common acute lymphoblastic leukemia antigen in vitro . J. Immunol. , 129 , 2287 – 2292 ( 1982. ). [PubMed] [Google Scholar]
  • 18. ) Lampson , L. A. and Levy , R.Two populations of la‐like molecules on a human B cell line . J. Immunol. , 125 , 293 – 299 ( 1980. ). [PubMed] [Google Scholar]
  • 19. ) Noro , N. , Yoshioka , A. , Adachi , M. , Yasuda , K. , Masuda , T. and Yodoi , J.Monoclonal antibody (H107) inhibiting IgE binding to FceER (+) human lymphocytes . J. Immunol. , 137 , 1258 – 1263 ( 1986. ). [PubMed] [Google Scholar]
  • 20. ) Tanaka , Y. , Tozawa , H. , Koyanagi , Y. , Yamamoto , N. and Hinuma , Y.A new monoclonal antibody recognizing an antigen of human lymphocytes similar or identical to Tac antigen . Microbiol. Immunol. , 28 , 1041 – 1055 ( 1984. ). [DOI] [PubMed] [Google Scholar]
  • 21. ) Ikewaki , N.Production of human B cell hybridomas with interleukin 2 receptors selected by a new selective medium, HAT‐neo . Kitasato Arch. Exp. Med. , 61 , 55 – 65 ( 1988. ). [PubMed] [Google Scholar]
  • 22. ) Laemmli , U. K.Cleavage of structural proteins during the assembly of the head of bacteriophage T4 . Nature , 227 , 680 – 685 ( 1970. ). [DOI] [PubMed] [Google Scholar]
  • 23. ) Towbin , H. , Staehelin , T. and Gordon , J.Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. Procedure and some application . Proc. Natl. Acad. Sci. USA , 76 , 4350 – 4354 ( 1979. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 24. ) Guesdon , J. P. , Ternynck , T. and Avrameas , S.The use of avidin‐biotin interaction in immunoenzymatic techniques . J. Histochem. Cytochem. , 27 , 1131 – 1139 ( 1979. ). [DOI] [PubMed] [Google Scholar]
  • 25. ) Mittal , K. K. , Mickey , M. R. , Singal , D. P. and Terasaki , P. I.Serotypying for homotransplantation. XVIII. Refinement of microdroplet lymphocyte cytotoxicity test . Transplantation , 6 , 913 – 927 ( 1968. ). [DOI] [PubMed] [Google Scholar]
  • 26. ) Minowada , J. , Koshiba , H. , Sagawa , K. , Kubonishi , I. , Lok , M. S. , Tatsumi , E. , Han , T. , Srivastava , B. I. S. and Ohnuma , T.Marker profiles of human leukemia and lymphoma cell lines . J. Cancer Res. Clin. Oncol. , 101 , 91 – 100 ( 1981. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 27. ) Carrel , S. , de Tribolet , N. and Gross , N.Expression of HLA‐DR and common acute lymphoblastic leukemia antigens on glioma cells . Eur. J. Immunol. , 12 , 354 – 357 ( 1982. ). [DOI] [PubMed] [Google Scholar]
  • 28. ) Hattori , S. , Imagawa , K. , Shimizu , F. , Hashimura , E. , Seiki , M. and Yoshida , M.Identification of envelope glycoprotein encoded by env gene of human T‐cell leukemia virus . Gann , 74 , 790 – 793 ( 1983. ). [PubMed] [Google Scholar]
  • 29. ) Miwa , M. , Shimotohno , K. , Hoshino , H. , Fujino , M. and Sugimura , T.Detection of pX proteins in human T‐cell leukemia virus (HTLV)‐infected cells by using antibody against peptide deduced from sequences of X‐IV DNA of HTLV‐I and Xc DNA of HTLV‐II proviruses . Gann , 75 , 752 – 755 ( 1984. ). [PubMed] [Google Scholar]
  • 30. ) Tanaka , Y. , Inoi , T. , Tozawa , H. , Yamamoto , N. and Hinuma , Y.A glycoprotein antigen detected with new monoclonal antibodies on the surface of human lymphocytes infected with human T‐cell leukemia virus type‐I (HTLV‐I) . Int. J. Cancer , 36 , 549 – 555 ( 1985. ). [DOI] [PubMed] [Google Scholar]

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