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. 2018 Mar 23;9(36):24260–24271. doi: 10.18632/oncotarget.24916

Figure 3. Effect of HX-1171 on STZ-induced cell apoptosis.

Figure 3

Cell apoptosis was analyzed by flow cytometry of cells double-stained with Annexin V and 7-AAD. (A) Flow cytometric dot plots, and (B) Annexin-V-positive/7-AAD-negative (early apoptotic) and total apoptosis of STZ and HX-1171-treated INS-1 cells. The cells were either untreated or treated with STZ (5 mM) and indicated dose of HX-1171 for 16 hrs. (C) Expression of apoptosis-related signal proteins was analyzed by western blotting. INS-1 cells were either untreated or treated with STZ (5 mM) and indicated doses of HX-1171 for 4 hrs. Protein levels were normalized to β-actin. Data are presented as mean ± SD (n = 3). **P < 0.005, ***P < 0.001 compared to the control group, #P < 0.05, ##P < 0.005 compared to the STZ group.