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. 2018 Mar 23;9(36):24260–24271. doi: 10.18632/oncotarget.24916

Figure 4. Effect of HX-1171 on STZ-induced intracellular ROS expression.

Figure 4

(A) Intracellular ROS levels and (B) Mean Fluorescence Intensity (M.F.I.) were analyzed by flow cytometry using DCFH-DA dye. Cells were either untreated or treated with STZ (5 mM) and HX-1171 (10 μM) for 16 hrs. (C) Expression of Nrf2-related antioxidant proteins was analyzed by western blotting. INS-1 cells were either untreated or treated with STZ (5 mM) and indicated doses of HX-1171 for 4 hrs. Protein levels were normalized to β-actin. Data are presented as mean ± SD (n = 3). **P < 0.005 compared to the control group, #P < 0.05, ##P < 0.005, ###P < 0.001 compared to the STZ group.