Extended Data Fig. 3. Overview of the IFT-B subunits functionalised with C-terminal tags and their photobleaching properties.

The respective subunits were functionalised either with a GFP- or with a SNAP-tag for fluorescence labeling. All subunits displayed mostly single-step photobleaching consistent with non-aggregated, single subunits after functionalisation. N represents the number of events obtained from three different slides from three independent experiments.