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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Traffic. 2017 Oct 23;18(12):825–839. doi: 10.1111/tra.12528

Figure 2.

Figure 2

SNARE cleavage using BoNT/C and BoNT/E does not affect DOC2B translocation. (A) Cells co-expressing BoNT/C and BoNT/E (BoNT/C+E) together with SNAP25-YFP or mRFP-syx1A. Fluorophores were placed at the site of the t-SNARE protein that is detached from the membrane and enters the cytosol following cleavage. Images indicate full cleavage by the toxins. (B) Representative epifluorescence images and (C) TIRFM quantification of KCl-induced DOC2B-GFP translocation in the presence or absence of BoNT/C+E (n = 28 in the no toxin group and 34 in the toxin group; t-test P > 0.1). (D) R-GECO change in fluorescence indicates that Ca2+ dynamics induced by KCl remains similar following SNARE cleavage (n = 20 in each group; t-test P > 0.1). Data presented as mean ± SEM. Scale bar = 10 μm.