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. Author manuscript; available in PMC: 2018 Jul 10.
Published in final edited form as: Nature. 2018 Jan 10;553(7687):165–170. doi: 10.1038/nature25192

Fig. 1. Progressive neurite outgrowth of the GABAergic DVB neuron in adult males.

Fig. 1

(a) DVB neuron schematic. (b) DVB visualized with lim-6int4::wCherry during adulthood in males and hermaphrodites (asterisk=PVT neuron, arrowheads=DVB neurites, scale bar=10 μm(true for all subsequence figures), n same as (c)). Presynaptic boutons visualized with presynaptic marker lim-6int4::gfp::rab-3. DVB neurite outgrowth quantified by (c) total neurite length and (d) number of neurite junctions (dot=one animal, magenta bar=median, and boxes=quartiles. Comparison using one-way ANOVA and post-hoc Tukey HSD, p-values and n shown). (e) Schematic of DVB and post-synaptic spicule-associated neurons and muscles in male tail. (f) Example demonstrating males with normal or protracted spicules (red line indicates spicule(n>10)). (g) Connectivity of DVB at adult stage inferred from electron micrographs8,11. Chemical synapses depicted as arrows, black arrows and neurons are sex-shared, red are hermaphrodite-specific, blue are male-specific. Behavioral output indicated for each sex.