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. 2018 Apr 20;209(2):489–506. doi: 10.1534/genetics.118.300904

Figure 6.

Figure 6

ChIP-PCR identifies a PP-1 binding site ∼500 bp upstream of the adv-1 ORF. (A) Diagram of predicted PP-1 binding sites within 1.5 kb upstream of adv-1 ORF, based on the motif depicted in Figure 2B. Arrows indicate 10 different PCR primer sets used to interrogate immunoprecipitated chromatin. (B) Agarose DNA gel showing results of ChIP-PCR with primer set #4. The remaining PCR results are included in Figure S5. Immunoprecipitation with Δpp-1(Ptef1-pp-1-v5; his-3) and Δpp-1(Pccg1-pp-1-gfp; his-3) strains was performed using α-V5 or α-GFP antibodies. α-mouse antibodies were used as a negative control. Whole-cell lysate and independent N. crassa genomic DNA were included as positive PCR controls, with a PCR reaction lacking DNA as an additional negative control. (C) The sequence of the PCR product in B. Primers highlighted in yellow correspond to primer set #4 in A, and predicted PP-1 binding sites are in red.