Table 2.
Strains and plasmids.
| Strains | Relevant characteristics | Source |
|---|---|---|
| E. coli BL21(DE3) | Wild type | Laboratory stock |
| E. coli K-12 MG1655 | Wild type | Laboratory stock |
| E. coli ΔsucA | E. coli K-12 MG1655 with deletion of sucA | This study |
| E. coli ΔsucAΔaceA | E. coli K-12 MG1655 with deletion of sucA and aceA | This study |
| E. coli ΔsucAΔaceA-pWSK | E. coli ΔsucAΔaceA harboring pWSK29 | This study |
| E. coli ΔsucAΔaceA-ido | E. coli ΔsucAΔaceA harboring pWSK-ido | This study |
| E. coli ΔsucAΔaceA-idoM | E. coli ΔsucAΔaceA harboring pWSK-ido M3 | This study |
| BL-ido | E. coli BL21(DE3) harboring pET-ido | This study |
| BL- idoM3 | E. coli BL21(DE3) harboring pET-ido M3 | This study |
| Plasmids | ||
| pET-His | Expression vector, Ampr | Laboratory stock |
| pWSK29 | Expression vector, Ampr | Laboratory stock |
| pKD3 | As a template for amplification of chloramphenicol resistance gene cassette, Ampr, Chlr | Laboratory stock |
| pWSK-ido | pWSK29 containing ido | This study |
| pWSK-ido M3 | pWSK29 containing idoM3 | This study |
| pET-ido | pET-His containing ido | Laboratory stock |
| pET-idoM3 | pET-His containing idoM3 | This study |