Figure 2.

A possible order of events in nuclear degradation in keratinocytes. Possible stages of nuclear degradation based on our and other's data. To begin, the nucleus is intact but is marked by phosphorylation of Lamin A/C (1). This targets an autophagolysosome (LC3-positive/LAMP2-positive body, orange) to that region of the nuclear lamina (2). The autophagolysosome removes some of the nuclear content, reducing nuclear size (3). Steps 1–3 are repeated iteratively until the nuclear lamina is sufficiently damaged to allow ingress of DNases. Then large scale degradation of the nuclear material occurs, potentially concomitant with further degradation of the nuclear lamina (5). Red colour denotes nuclear material, while green denotes the nuclear lamina.