This article has been corrected: The correct Materials and Methods and Figure 2 are given below: The authors declare that these corrections do not change the results or conclusions of this paper.
Figure 2.
(A) MTT assay results showed that Aβ inhibited the viability of SH-SY5Y cells and downregulation of BACE1 enhanced the inhibitory effects of Aβ; (B) downregulation of miR-124 relieved Aβ-induced viability inhibition of SH-SY5Y cells; (C) flow cytometric analysis results showed that Aβ-induced apoptosis of SH-SY5Y cells and downregulation of BACE1 enhanced the induced effects of Aβ; (D) downregulation of miR-124 decreased apoptosis of SH-SY5Y cells in the presence of Aβ.
Luciferase reporting assay
The 3’ UTR of BACE1 and the CMV promoter were amplified from human chromosomal DNA and pcDNA3.1 (+) and cloned into the pGL3-luciferase basic vector (Promega, Madison, WI, USA). Sequences of primers and cloning strategy are available on request. For the luciferase assays, 50 nM of miR-124 mimics or scrambled RNA were co-transfected with the reporter vector and the Renilla control vector (Promega, Madison, WI, USA) into the HEK293 cells by Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA). 24 h post transfection, the measurements were performed using the Dual luciferase re-porter assay kit (Promega, Madison, WI, USA). Or the HEK293 cells post the transfection for 24 h was lyzed for western blot analysis.
Original article: Oncotarget. 2017; 8:114065-114071. https://doi.org/10.18632/oncotarget.23119