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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Neuropharmacology. 2018 Mar 23;135:431–443. doi: 10.1016/j.neuropharm.2018.03.019

Figure 4. Cocaine-mediated phosphorylation and nuclear localization of pIRF3 is reduced with antioxidants, PPF or PIO cotreatments.

Figure 4

NHA were treated with cocaine (0.1 μM) in the presence or absence of PPF (10 μM) or PIO (1 μM). Astrocytes treated with cocaine +/− PPF or PIO were fixed and fluorescently labeled with antibodies specific to IRF3 (green, A), pIRF3 (green, B-E) or GFAP (red) (C-E). Representative donors chosen from multiple biological replicates were tested, each analyzed in a minimum of triplicate determinations.