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. 2018 Apr 27;9(32):22301–22315. doi: 10.18632/oncotarget.24737

Figure 3. Triptolide arrests HeLa cell cycle through HSP90β.

Figure 3

(A) TL induces cell cycle arrest of HeLa at G1/G0 phase in a dosage dependent manner. Flow cytometry analysis (left) of cell cycle profiles of HeLa cells upon 24 hrs’ TL treatment (10, 30 and 100 nM). *P < 0.05; ***P < 0.001 compared with vehicle control. Data are represented as mean ± SD (n = 3). Western blot analysis (right) of cell cycle related proteins in HeLa cells at various TL concentrations for 24 hrs. Representative image from three independent experiment was shown. (B) Knockdown efficiency of HSP90β by siRNA. mRNA (upper) and protein (lower) level of HSP90β in HeLa cells upon transfection either with control siRNA or HSP90β siRNA was measured. Data are represented as mean ± SD (n = 3). (C) Knockdown of HSP90β sensitizes TL's cell cycle arrest effect on HeLa cells. Flow cytometry analysis (upper) of cell cycle profiles of HeLa cells transfected either with control siRNA or HSP90β siRNA in the presence and absence of TL for 24 hrs. *P < 0.05; *** P < 0.001 vehicle versus TL. ##P < 0.01 ctrl siRNA+TL versus HSP90β siRNA+TL. Data are presented as mean ± SD (n = 3). Western blot analysis (lower) of cell cycle-related proteins in HeLa cells transfected either with control siRNA or HSP90β siRNA in the presence and absence of TL for 24 hrs. Representative image from three independent experiment was shown.