TABLE 2 .
Overview of biofilm-related phenotypes of select SagS variants
| Substitution | Restoration of ΔsagS biofilm-related phenotypes to wild-type levelsa |
|||||
|---|---|---|---|---|---|---|
| Attachment | Biofilm architecture |
BfiS phosphorylation |
Susceptibility to tobramycin |
Tolerance to tobramycin |
BrlR levels |
|
| D105 | Yes | Yes | Yes | No | No | No |
| L126 | Yes | Yes | Yes | No | No | No |
| F131 | No | No | No | Yes | Yes | Yes |
| L154 | No | No | No | Yes | Yes | Yes |
| L141 | Yes | Yes | Yes | Intermediate | Yes | Yes |
| R116 | Intermediate | Yes | Yes | Intermediate | No | No |
a
The wild type and ΔsagS mutant strain harboring the empty vector pMJT-1 or overexpressing sagS were used as controls. Data correspond to results shown in Fig. 3 to 7. “Yes” indicates restoration of ΔsagS biofilm-related phenotypes to wild-type levels, “No” indicates no restoration to wild-type levels, and “Intermediate” indicates values or phenotypes were in between those obtained for the PAO1 wild type or ΔsagS/pMJT-sagS and ΔsagS mutants.